Efecto biofertilizante del preparado: residuos vegetales -bacteria nativa diazótrofa, sobre las variables biométricas en plántulas de Rhapanus sativus
<p style="text-align: center;" align="center"><span class="hps"><strong style="mso-bidi-font-weight: normal;"><span style="color: black; mso-ansi-language: EN-US;" lang="EN-US"><span style="font-family: Times...
| Main Authors: | , , |
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| Format: | Article |
| Language: | Spanish |
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Universidad Nacional de Colombia
2011-04-01
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| Series: | Revista Colombiana de Biotecnología |
| Subjects: | |
| Online Access: | http://www.revistas.unal.edu.co/index.php/biotecnologia/article/view/22989 |
| Summary: | <p style="text-align: center;" align="center"><span class="hps"><strong style="mso-bidi-font-weight: normal;"><span style="color: black; mso-ansi-language: EN-US;" lang="EN-US"><span style="font-family: Times New Roman;"><span style="font-size: small;">Biofertilizer effect of the prepared from vegetales wastes -diazotroph native bacterium on biometrics variables of <em style="mso-bidi-font-style: normal;">Rhapanus sativus</em> seedlings</span></span></span></strong></span></p><p class="MsoNormal" style="text-align: center; line-height: normal; margin: 0cm 0cm 0pt;" align="center"> </p><p class="MsoNormal" style="text-align: justify; line-height: normal; margin: 0cm 0cm 0pt; tab-stops: 193.95pt;"><strong style="mso-bidi-font-weight: normal;"><span style="font-family: " lang="ES">RESUMEN<span style="mso-tab-count: 1;"> </span></span></strong></p><p class="MsoNormal" style="text-align: justify; line-height: normal; margin: 0cm 0cm 0pt;"><span style="font-family: ">El uso de bioinoculantes a base de microorganismos con potencial biofertilizante representa una alternativa económicamente viable y de producción limpia para el sector agrícola. </span><span style="font-family: " lang="ES-AR">El objetivo del presente trabajo fue evaluar el efecto biofertilizante de un preparado </span><span style="font-family: ">elaborado con residuos sólidos vegetales</span><span style="font-family: "> </span><span style="font-family: ">(RSV) </span><span style="font-family: ">procedentes del mercado y </span><span style="font-family: " lang="ES-AR">la bacteria nativa diazótrofa </span><em style="mso-bidi-font-style: normal;"><span style="font-family: ">Azotobacter </span></em><span style="font-family: ">A15M2G</span><span style="font-family: ">. Se elaboraron biopreparados utilizando diferentes concentraciones de bacteria (</span><span style="font-family: ">10<sup>6</sup>, 10<sup>7</sup> y 10<sup>8 </sup>UFC) en un medio de cultivo obtenido a partir del 25% p/v de cada uno de los siguientes RSV: <em>Brassica oleracea</em> (repollo), <em>Lactuca sativa</em> (lechuga) y <em>Allium fistulosum</em> (cebollín).</span><span style="font-family: "> Los biopreparados fueron evaluados en plantas de </span><span style="font-family: ">rábano (</span><em style="mso-bidi-font-style: normal;"><span style="font-family: ">Rhapanus sativus</span></em><em style="mso-bidi-font-style: normal;"><span style="font-family: ">) </span></em><span style="font-family: ">en invernadero, utilizando un diseño estadístico completamente al azar de 5 tratamientos con 3 repeticiones: T1, control; T2, semillas pregerminadas tratadas con </span><span style="font-family: ">RSV</span><span style="font-family: "> al 25% p/v; T3, semillas pregerminadas con bioinoculante de 10<sup>6 </sup>UFC; T4, semillas pregerminadas con bioinoculante de 10<sup>7 </sup>UFC; T5, semillas pregerminadas con bioinoculante de 10<sup>8 </sup>UFC. Se evaluó: número de hojas, área foliar, longitud de la planta, longitud de la raíz y peso seco de toda la planta (ensayos por triplicado). Se observó un incremento altamente significativo en peso seco para </span><span style="font-family: ">T5 (0,88 g) y T4 (1,10 g); </span><span style="font-family: ">y diferencias significativas en el área foliar, para los mismos tratamientos, </span><span style="font-family: ">con un valor superior a 2000 cm<sup>2</sup>.<sup> </sup>El biopreparado con bacterias nativas y RSV mejoró el crecimiento y desarrollo de las plantas de rábano, pudiéndose dar </span><span style="font-family: " lang="ES-AR">un valor agregado a estos residuos y de esta manera obtener un biofertilizante potencialmente utilizable en otros cultivos.</span></p><p class="MsoNormal" style="text-align: justify; line-height: normal; margin: 0cm 0cm 0pt;"><span style="font-family: " lang="ES-AR"> </span></p><p class="MsoNormal" style="text-align: justify; line-height: normal; margin: 0cm 0cm 0pt;"><strong style="mso-bidi-font-weight: normal;"><span style="font-family: " lang="EN-US">Palabras clave</span></strong><span style="font-family: " lang="EN-US">:<em style="mso-bidi-font-style: normal;"> Azotobacter </em>A15M2G<em style="mso-bidi-font-style: normal;">, <span style="mso-bidi-font-style: italic;">Brassica oleracea</span></em>, <em>Lactuca sativa</em><span style="mso-bidi-font-style: italic;">,</span> <em>Allium fistulosum</em>, bioinoculante. <em style="mso-bidi-font-style: normal;"></em></span></p><p class="MsoNormal" style="text-align: justify; line-height: normal; margin: 0cm 0cm 0pt;"><strong style="mso-bidi-font-weight: normal;"><span style="font-family: " lang="EN-US"> </span></strong></p><p class="MsoNormal" style="text-align: justify; line-height: normal; margin: 0cm 0cm 0pt;"><strong style="mso-bidi-font-weight: normal;"><span style="font-family: " lang="EN-US">ABSTRACT</span></strong></p><p class="MsoNormal" style="text-align: justify; line-height: normal; margin: 0cm 0cm 0pt; vertical-align: top;"><span class="hps"><span style="color: black; font-size: 12pt; mso-ansi-language: EN-US;" lang="EN-US"><span style="font-family: Times New Roman;">The use of bioinoculantes from microorganisms with biofertilizer potential, represents an economically viable alternative and of clean production for the agricultural sector. The aim of this study</span></span></span><span style="font-family: " lang="EN-US"> <span class="hps">was</span> <span class="hps">to evaluate</span> <span class="hps">the</span> <span class="hps">effect</span> <span class="hps">of</span> <span class="hps">biofertilizer</span> <span class="hps">preparation obtained</span> <span class="hps">from</span> </span><span style="font-family: " lang="EN-US">vegetable solid waste (RSV) of <span class="hps"><span style="color: black;">the</span></span><span style="color: black;"> <span class="hps">market and</span> <span class="hps">the</span> <span class="hps">native</span> <span class="hps">bacteria</span> <span class="hps"><em style="mso-bidi-font-style: normal;">Azotobacter</em></span><em style="mso-bidi-font-style: normal;"> </em><span class="hps">A15M2G diazotroph</span>. </span></span></p><p class="MsoNormal" style="line-height: normal; margin: 0cm 0cm 0pt; vertical-align: top;"><span class="hps"><span style="color: black; font-size: 12pt; mso-ansi-language: EN-US;" lang="EN-US"><span style="font-family: Times New Roman;">Biological cultures</span></span></span><span style="font-family: " lang="EN-US"> <span class="hps">were prepared</span> <span class="hps">using</span> <span class="hps">different inoculum concentrations</span>, </span><span style="font-family: " lang="EN-US">10<sup>6</sup>, 10<sup>7</sup> y 10<sup>8 </sup>UFC <span class="hps"><span style="color: black;">in</span></span><span style="color: black;"> <span class="hps">a culture medium</span> <span class="hps">obtained</span> <span class="hps">from 25</span>% <span class="hps">w</span> <span class="hps">/</span> <span class="hps">v</span> <span class="hps">of</span> <span class="hps">each</span> <span class="hps">of</span> <span class="hps">the</span> <span class="hps">following</span> <span class="hps">substrates</span></span>: <em>Brassica oleracea</em> (cabbage), <em>Lactuca sativa</em> (lettuce) and <em>Allium fistulosum</em> (chives)<span style="color: black;">. <span class="hps">The</span> <span class="hps">microbial inoculants were evaluated</span> <span class="hps">in</span> <span class="hps">radish plants</span> <span class="hpsatn">(</span><em style="mso-bidi-font-style: normal;">Rhapanus <span class="hps">sativus</span></em>) </span><span class="hps"><span style="color: black; mso-themecolor: text1;">in greenhouse</span><span style="color: red;"> </span><span style="color: black;">using</span></span><span style="color: black;"> <span class="hps">a</span> <span class="hps">completely randomized</span> <span class="hps">design</span> <span class="hps">of</span> <span class="hps">5</span> <span class="hps">treatments</span> <span class="hps">with</span> <span class="hps">3</span> <span class="hps">replicates</span>: T1, <span class="hps">pre-germinated</span> <span class="hps">seeds</span> <span class="hps">without any</span> <span class="hps">treatment</span>; T2, <span class="hps">pre-germinated</span> <span class="hps">seeds</span> <span class="hps">treated</span> <span class="hps">with the dye</span> <span class="hps">waste</span> <span class="hps">vegetables</span> <span class="hps">25%</span> <span class="hps">w</span> <span class="hps">/</span> <span class="hps">v</span>; T3, <span class="hps">pre-germinated</span> <span class="hps">seeds</span> <span class="hps">treated</span> <span class="hps">with</span> <span class="hps">bacterial</span> <span class="hps">concentration</span> <span class="hps">bioinoculants</span> <span class="hps">to</span> </span></span><span style="font-family: " lang="EN-US">10<sup>6 </sup>UFC</span><span style="font-family: " lang="EN-US">; T4, <span class="hps">pre-germinated</span> <span class="hps">seeds</span> <span class="hps">treated</span> <span class="hps">with</span> <span class="hps">bacterial</span> <span class="hps">concentration</span> <span class="hps">bioinoculants</span> <span class="hps">to</span> </span><span style="font-family: " lang="EN-US">10<sup>7 </sup>UFC</span><span style="font-family: " lang="EN-US">, and T5, <span class="hps">pre-germinated</span> <span class="hps">seeds</span> <span class="hps">treated</span> <span class="hps">with</span> <span class="hps">bacterial</span> <span class="hps">concentration</span> <span class="hps">bioinoculants</span> <span class="hps">to</span> </span><span style="font-family: " lang="EN-US">10<sup>8 </sup>UFC</span><span style="font-family: " lang="EN-US">. </span><span style="font-family: " lang="EN-US">Assessed variables <span class="longtext"><span style="background: white;">were: number of leaves, leaf area, plant length, root length and dry weight of the entire plant (all assays in triplicate). The results showed a highly significant increase in dry weight, for T5 (0.88 g) and T4(1.10 g); and significant differences in leaf area for the same treatments, with a value greater than 2000 cm2, compared to others.</span></span></span><span style="font-size: small;"><span style="mso-ansi-language: EN-US;" lang="EN-US"><span style="font-family: Calibri;"> </span></span><span class="longtext"><span style="font-family: " lang="EN-US">The biopreparado from native bacteria and RSV improved the growth and development of the radish plants, being able to give a added value to these residues and to obtain a potentially usable biofertilizer in other cultures.</span></span></span></p><p class="MsoNormal" style="text-align: justify; line-height: normal; margin: 0cm 0cm 0pt; vertical-align: top;"><span class="hps"><strong style="mso-bidi-font-weight: normal;"><span style="color: black; font-size: 12pt; mso-ansi-language: PT;" lang="PT"><span style="font-family: Times New Roman;"> </span></span></strong></span></p><span style="font-family: Times New Roman;"><span class="hps"><strong style="mso-bidi-font-weight: normal;"><span style="line-height: 115%; color: black; font-size: 12pt; mso-fareast-font-family: Calibri; mso-ansi-language: PT; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;" lang="PT">Key words</span></strong></span><strong style="mso-bidi-font-weight: normal;"><span style="line-height: 115%; font-family: " lang="PT">:</span></strong><span style="line-height: 115%; font-family: " lang="PT"> <span class="hps"><em style="mso-bidi-font-style: normal;">Azotobacte</em>r</span> <span class="hps">A15M2G</span>, </span><em><span style="line-height: 115%; font-family: " lang="PT">Brassica oleracea</span></em><span style="line-height: 115%; font-family: " lang="PT">, <em>Lactuca sativa</em><span style="mso-bidi-font-style: italic;">,</span> <em>Allium fistulosum</em>, <span class="hps"><span style="color: black;">bioinoculants</span></span><span style="color: black;">.</span></span><span style="font-size: small;"> </span></span> |
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| ISSN: | 0123-3475 1909-8758 |