Genetic differentiation of Hippa adactyla based on CO1 gene sequence in Sundaland
The Hippidae is a family of sand crabs that inhabit intertidal ecosystems, specifically in sandy areas scattered throughout the coast. Differences in habitat conditions cause variations in Hippidae members. Hippa adactyla is a member of the genus Hippa. This study aimed to map the genetic differenti...
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| Format: | Article |
| Language: | English |
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EDP Sciences
2024-01-01
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| Series: | BIO Web of Conferences |
| Online Access: | https://www.bio-conferences.org/articles/bioconf/pdf/2024/13/bioconf_icbs2024_04011.pdf |
| _version_ | 1797235342737145856 |
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| author | Eko Susilo Vendi Windy Putri Vinna Alfiyan Achmad Mashar Ali Wardiatno Yusli Farajalah Achmad |
| author_facet | Eko Susilo Vendi Windy Putri Vinna Alfiyan Achmad Mashar Ali Wardiatno Yusli Farajalah Achmad |
| author_sort | Eko Susilo Vendi |
| collection | DOAJ |
| description | The Hippidae is a family of sand crabs that inhabit intertidal ecosystems, specifically in sandy areas scattered throughout the coast. Differences in habitat conditions cause variations in Hippidae members. Hippa adactyla is a member of the genus Hippa. This study aimed to map the genetic differentiation between Hippa adactyla populations in the Greater Sunda Islands. The sand crab (Hippa adactyla) samples were taken from Sumatra (Pariaman, Bengkulu, Lampung) and Java (Pangandaran, Pelabuhan Ratu, Banyuwangi) by handcatching then stored in alcohol 96%. Total genomic DNA was extracted from the muscle tissue of the samples using a commercial DNA Extraction KIT. The upper part of the COI gene (5' end) (±750 bp) was amplified using specific primers. Based on the K2P substitution model, the genetic distance of the Pangandaran population to the Bali Strait population is the same as that of the Sumatran population (0.062). Genetic diversity ranges from 0.000 to 0.123. Haplotype diversity was 0.928, consisting of 18 haplotypes. |
| first_indexed | 2024-04-24T16:46:26Z |
| format | Article |
| id | doaj.art-8b760f0d2e65467fa32a0a524cb96f19 |
| institution | Directory Open Access Journal |
| issn | 2117-4458 |
| language | English |
| last_indexed | 2024-04-24T16:46:26Z |
| publishDate | 2024-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | BIO Web of Conferences |
| spelling | doaj.art-8b760f0d2e65467fa32a0a524cb96f192024-03-29T08:27:50ZengEDP SciencesBIO Web of Conferences2117-44582024-01-01940401110.1051/bioconf/20249404011bioconf_icbs2024_04011Genetic differentiation of Hippa adactyla based on CO1 gene sequence in SundalandEko Susilo Vendi0Windy Putri Vinna1Alfiyan Achmad2Mashar Ali3Wardiatno Yusli4Farajalah Achmad5Animal Bioscience Study Program, Department of Biology, Faculty of Mathematics and Natural Sciences, IPB University, Kampus IPB DramagaAnimal Bioscience Study Program, Department of Biology, Faculty of Mathematics and Natural Sciences, IPB University, Kampus IPB DramagaAnimal Bioscience Study Program, Department of Biology, Faculty of Mathematics and Natural Sciences, IPB University, Kampus IPB DramagaDepartment of Aquatic Resources Management, Faculty of Fisheries and Marine Sciences, IPB University, Kampus IPB DramagaDepartment of Aquatic Resources Management, Faculty of Fisheries and Marine Sciences, IPB University, Kampus IPB DramagaAnimal Bioscience Study Program, Department of Biology, Faculty of Mathematics and Natural Sciences, IPB University, Kampus IPB DramagaThe Hippidae is a family of sand crabs that inhabit intertidal ecosystems, specifically in sandy areas scattered throughout the coast. Differences in habitat conditions cause variations in Hippidae members. Hippa adactyla is a member of the genus Hippa. This study aimed to map the genetic differentiation between Hippa adactyla populations in the Greater Sunda Islands. The sand crab (Hippa adactyla) samples were taken from Sumatra (Pariaman, Bengkulu, Lampung) and Java (Pangandaran, Pelabuhan Ratu, Banyuwangi) by handcatching then stored in alcohol 96%. Total genomic DNA was extracted from the muscle tissue of the samples using a commercial DNA Extraction KIT. The upper part of the COI gene (5' end) (±750 bp) was amplified using specific primers. Based on the K2P substitution model, the genetic distance of the Pangandaran population to the Bali Strait population is the same as that of the Sumatran population (0.062). Genetic diversity ranges from 0.000 to 0.123. Haplotype diversity was 0.928, consisting of 18 haplotypes.https://www.bio-conferences.org/articles/bioconf/pdf/2024/13/bioconf_icbs2024_04011.pdf |
| spellingShingle | Eko Susilo Vendi Windy Putri Vinna Alfiyan Achmad Mashar Ali Wardiatno Yusli Farajalah Achmad Genetic differentiation of Hippa adactyla based on CO1 gene sequence in Sundaland BIO Web of Conferences |
| title | Genetic differentiation of Hippa adactyla based on CO1 gene sequence in Sundaland |
| title_full | Genetic differentiation of Hippa adactyla based on CO1 gene sequence in Sundaland |
| title_fullStr | Genetic differentiation of Hippa adactyla based on CO1 gene sequence in Sundaland |
| title_full_unstemmed | Genetic differentiation of Hippa adactyla based on CO1 gene sequence in Sundaland |
| title_short | Genetic differentiation of Hippa adactyla based on CO1 gene sequence in Sundaland |
| title_sort | genetic differentiation of hippa adactyla based on co1 gene sequence in sundaland |
| url | https://www.bio-conferences.org/articles/bioconf/pdf/2024/13/bioconf_icbs2024_04011.pdf |
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