Transplantation impacts on the oral microbiome of kidney recipients and donors
IntroductionChronic kidney disease (CKD) may affect the human microbiome via increased concentrations of uremic toxins such as urea and creatinine. MethodsWe have profiled the oral microbiota in patients with CKD before and one week after kidney transplantation. Living kidney donors were also longit...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-11-01
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| Series: | Frontiers in Microbiomes |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/frmbi.2023.1258290/full |
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| author | Paul M. Campbell Thomas Willmott Gavin J. Humphreys Oana Piscoran Houda Chea Angela M. Summers Joanne E. Konkel Christopher G. Knight Titus Augustine Andrew J. McBain |
| author_facet | Paul M. Campbell Thomas Willmott Gavin J. Humphreys Oana Piscoran Houda Chea Angela M. Summers Joanne E. Konkel Christopher G. Knight Titus Augustine Andrew J. McBain |
| author_sort | Paul M. Campbell |
| collection | DOAJ |
| description | IntroductionChronic kidney disease (CKD) may affect the human microbiome via increased concentrations of uremic toxins such as urea and creatinine. MethodsWe have profiled the oral microbiota in patients with CKD before and one week after kidney transplantation. Living kidney donors were also longitudinally tracked over a similar period, allowing direct comparison between a group undergoing transplant surgery alone (donors) (n=13) and a group additionally undergoing the introduction of immunosuppressive agents and the resolution of CKD (recipients) (n=45). ResultsTransplantation was associated with a similar pattern of decreasing alpha diversity in the oral microbiome in recipients and donors via Kruskal-Wallis testing, within one week of transplantation. Amplicon sequence variants (ASVs) associated with Haemophilus parainfluenzae, Aggregatibacteria segnis, Peptostreptococcus and Actinobacillus were significantly decreased in recipients within a week of transplantation.DiscussionA reduction in ASVs in these genera could influence the risk of bacterial endocarditis, a rare but high-mortality kidney transplantation complication. A range of factors may drive the observed changes in oral microbiome including both factors associated with surgery itself and the decreases in salivary urea, administration of macrolide antibiotic immunosuppressants, and disruption to immune function that characterise kidney transplant. |
| first_indexed | 2024-03-11T13:22:35Z |
| format | Article |
| id | doaj.art-8b7c3b3f41fa4d68a2c8bd18d4ea69bb |
| institution | Directory Open Access Journal |
| issn | 2813-4338 |
| language | English |
| last_indexed | 2024-03-11T13:22:35Z |
| publishDate | 2023-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiomes |
| spelling | doaj.art-8b7c3b3f41fa4d68a2c8bd18d4ea69bb2023-11-03T10:26:40ZengFrontiers Media S.A.Frontiers in Microbiomes2813-43382023-11-01210.3389/frmbi.2023.12582901258290Transplantation impacts on the oral microbiome of kidney recipients and donorsPaul M. Campbell0Thomas Willmott1Gavin J. Humphreys2Oana Piscoran3Houda Chea4Angela M. Summers5Joanne E. Konkel6Christopher G. Knight7Titus Augustine8Andrew J. McBain9School of Health Sciences, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester, United KingdomSchool of Health Sciences, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester, United KingdomSchool of Health Sciences, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester, United KingdomManchester Centre for Transplantation, Manchester Royal Infirmary, Manchester University NHS Foundation Trust, Manchester Academic Health Science Centre, Manchester, United KingdomManchester Centre for Transplantation, Manchester Royal Infirmary, Manchester University NHS Foundation Trust, Manchester Academic Health Science Centre, Manchester, United KingdomManchester Centre for Transplantation, Manchester Royal Infirmary, Manchester University NHS Foundation Trust, Manchester Academic Health Science Centre, Manchester, United KingdomLydia Becker Institute of Immunology and Inflammation, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester, United KingdomSchool of Natural Sciences, Faculty of Science and Engineering, The University of Manchester, Manchester, United KingdomManchester Centre for Transplantation, Manchester Royal Infirmary, Manchester University NHS Foundation Trust, Manchester Academic Health Science Centre, Manchester, United KingdomSchool of Health Sciences, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester, United KingdomIntroductionChronic kidney disease (CKD) may affect the human microbiome via increased concentrations of uremic toxins such as urea and creatinine. MethodsWe have profiled the oral microbiota in patients with CKD before and one week after kidney transplantation. Living kidney donors were also longitudinally tracked over a similar period, allowing direct comparison between a group undergoing transplant surgery alone (donors) (n=13) and a group additionally undergoing the introduction of immunosuppressive agents and the resolution of CKD (recipients) (n=45). ResultsTransplantation was associated with a similar pattern of decreasing alpha diversity in the oral microbiome in recipients and donors via Kruskal-Wallis testing, within one week of transplantation. Amplicon sequence variants (ASVs) associated with Haemophilus parainfluenzae, Aggregatibacteria segnis, Peptostreptococcus and Actinobacillus were significantly decreased in recipients within a week of transplantation.DiscussionA reduction in ASVs in these genera could influence the risk of bacterial endocarditis, a rare but high-mortality kidney transplantation complication. A range of factors may drive the observed changes in oral microbiome including both factors associated with surgery itself and the decreases in salivary urea, administration of macrolide antibiotic immunosuppressants, and disruption to immune function that characterise kidney transplant.https://www.frontiersin.org/articles/10.3389/frmbi.2023.1258290/fulloral microbiomekidney transplantimmunosuppressionsurgeryrenal allograftchronic kidney disease |
| spellingShingle | Paul M. Campbell Thomas Willmott Gavin J. Humphreys Oana Piscoran Houda Chea Angela M. Summers Joanne E. Konkel Christopher G. Knight Titus Augustine Andrew J. McBain Transplantation impacts on the oral microbiome of kidney recipients and donors Frontiers in Microbiomes oral microbiome kidney transplant immunosuppression surgery renal allograft chronic kidney disease |
| title | Transplantation impacts on the oral microbiome of kidney recipients and donors |
| title_full | Transplantation impacts on the oral microbiome of kidney recipients and donors |
| title_fullStr | Transplantation impacts on the oral microbiome of kidney recipients and donors |
| title_full_unstemmed | Transplantation impacts on the oral microbiome of kidney recipients and donors |
| title_short | Transplantation impacts on the oral microbiome of kidney recipients and donors |
| title_sort | transplantation impacts on the oral microbiome of kidney recipients and donors |
| topic | oral microbiome kidney transplant immunosuppression surgery renal allograft chronic kidney disease |
| url | https://www.frontiersin.org/articles/10.3389/frmbi.2023.1258290/full |
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