Effects of Probiotics on the Expression and Localization of Avian β-defensins in the Proventriculus of Broiler Chicks
The aim of this study was to determine the effects of probiotics-feeding on the gene expression and protein localization of avian β-defensins (AvBDs) in the proventriculus of broiler chicks. Male broiler chicks were arranged in 3 groups: control group, probiotics group I and probiotics group II, wh...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Japan Poultry Science Association
2015-01-01
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| Series: | The Journal of Poultry Science |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jpsa/52/1/52_0140114/_pdf/-char/en |
| _version_ | 1827938950670450688 |
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| author | Elsayed S. I. Mohammed Yasumi Igarashi Naoki Isobe Yukinori Yoshimura |
| author_facet | Elsayed S. I. Mohammed Yasumi Igarashi Naoki Isobe Yukinori Yoshimura |
| author_sort | Elsayed S. I. Mohammed |
| collection | DOAJ |
| description | The aim of this study was to determine the effects of probiotics-feeding on the gene expression and protein localization of avian β-defensins (AvBDs) in the proventriculus of broiler chicks. Male broiler chicks were arranged in 3 groups: control group, probiotics group I and probiotics group II, which were fed with starter rations containing 0%, 0.2% or 0.4% probiotics, respectively, from day 0 (D0; at one day old) to D14. Proventriculi in all groups were collected at D0, D7 and D14 for analysis of AvBDs expression and AvBD12 protein localization. The expression of AvBDs genes was examined by reverse transcription-PCR and changes in the expression upon probiotics-feeding were examined by real-time PCR. The AvBD12 localization was examined by immunohistochemistry, and density of immunoreaction products was examined by image analysis under a microscope. Out of 14 AvBDs genes, seven AvBDs were detected in the proventriculus of chicks, namely, AvBD1, 2, 4, 6, 7, 10 and 12. The expression of the 7 detected genes did not show any significant differences between control and probiotics groups at D7 and D14. The immunoreactive (ir) -AvBD12 was localized in surface epithelium and cells in the connective tissues of proventricular glands. The ir-AvBD12 density in surface epithelium was significantly higher at D7 than at D0 or D14 in control group. At D7 and D14, the ir-AvBD12 density was significantly lower in probiotics groups than in control group. The ir-AvBD12 cells in proventricular gland increased in number with age; however, there were no significant differences between control and probiotics groups at D7 and D14. These results suggest that, although probiotics-feeding does not affect the gene expression of AvBDs, it may induce AvBD12 secretion from the surface epithelium of the proventriculus in broiler chicks. |
| first_indexed | 2024-03-13T08:46:14Z |
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| id | doaj.art-8b7d2123c7b0495580f52ea4fb4e23e7 |
| institution | Directory Open Access Journal |
| issn | 1346-7395 1349-0486 |
| language | English |
| last_indexed | 2024-03-13T08:46:14Z |
| publishDate | 2015-01-01 |
| publisher | Japan Poultry Science Association |
| record_format | Article |
| series | The Journal of Poultry Science |
| spelling | doaj.art-8b7d2123c7b0495580f52ea4fb4e23e72023-05-30T04:41:34ZengJapan Poultry Science AssociationThe Journal of Poultry Science1346-73951349-04862015-01-01521576710.2141/jpsa.0140114jpsaEffects of Probiotics on the Expression and Localization of Avian β-defensins in the Proventriculus of Broiler ChicksElsayed S. I. Mohammed0Yasumi Igarashi1Naoki Isobe2Yukinori Yoshimura3Graduate School of Biosphere Science, Hiroshima University, JapanToa Pharmaceutical Co., Ltd., JapanGraduate School of Biosphere Science, Hiroshima University, JapanGraduate School of Biosphere Science, Hiroshima University, JapanThe aim of this study was to determine the effects of probiotics-feeding on the gene expression and protein localization of avian β-defensins (AvBDs) in the proventriculus of broiler chicks. Male broiler chicks were arranged in 3 groups: control group, probiotics group I and probiotics group II, which were fed with starter rations containing 0%, 0.2% or 0.4% probiotics, respectively, from day 0 (D0; at one day old) to D14. Proventriculi in all groups were collected at D0, D7 and D14 for analysis of AvBDs expression and AvBD12 protein localization. The expression of AvBDs genes was examined by reverse transcription-PCR and changes in the expression upon probiotics-feeding were examined by real-time PCR. The AvBD12 localization was examined by immunohistochemistry, and density of immunoreaction products was examined by image analysis under a microscope. Out of 14 AvBDs genes, seven AvBDs were detected in the proventriculus of chicks, namely, AvBD1, 2, 4, 6, 7, 10 and 12. The expression of the 7 detected genes did not show any significant differences between control and probiotics groups at D7 and D14. The immunoreactive (ir) -AvBD12 was localized in surface epithelium and cells in the connective tissues of proventricular glands. The ir-AvBD12 density in surface epithelium was significantly higher at D7 than at D0 or D14 in control group. At D7 and D14, the ir-AvBD12 density was significantly lower in probiotics groups than in control group. The ir-AvBD12 cells in proventricular gland increased in number with age; however, there were no significant differences between control and probiotics groups at D7 and D14. These results suggest that, although probiotics-feeding does not affect the gene expression of AvBDs, it may induce AvBD12 secretion from the surface epithelium of the proventriculus in broiler chicks.https://www.jstage.jst.go.jp/article/jpsa/52/1/52_0140114/_pdf/-char/enavian β-defensinschick proventriculusinnate immunityprobiotics |
| spellingShingle | Elsayed S. I. Mohammed Yasumi Igarashi Naoki Isobe Yukinori Yoshimura Effects of Probiotics on the Expression and Localization of Avian β-defensins in the Proventriculus of Broiler Chicks The Journal of Poultry Science avian β-defensins chick proventriculus innate immunity probiotics |
| title | Effects of Probiotics on the Expression and Localization of Avian β-defensins in the Proventriculus of Broiler Chicks |
| title_full | Effects of Probiotics on the Expression and Localization of Avian β-defensins in the Proventriculus of Broiler Chicks |
| title_fullStr | Effects of Probiotics on the Expression and Localization of Avian β-defensins in the Proventriculus of Broiler Chicks |
| title_full_unstemmed | Effects of Probiotics on the Expression and Localization of Avian β-defensins in the Proventriculus of Broiler Chicks |
| title_short | Effects of Probiotics on the Expression and Localization of Avian β-defensins in the Proventriculus of Broiler Chicks |
| title_sort | effects of probiotics on the expression and localization of avian β defensins in the proventriculus of broiler chicks |
| topic | avian β-defensins chick proventriculus innate immunity probiotics |
| url | https://www.jstage.jst.go.jp/article/jpsa/52/1/52_0140114/_pdf/-char/en |
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