Hydrocarbon-Stapled Peptide Based-Nanoparticles for siRNA Delivery
Small interfering RNAs (siRNAs) are promising molecules for developing new therapies based on gene silencing; however, their delivery into cells remains an issue. In this study, we took advantage of stapled peptide technology that has emerged as a valuable strategy to render natural peptides more st...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-11-01
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| Series: | Nanomaterials |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-4991/10/12/2334 |
| _version_ | 1797546853340807168 |
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| author | Matthieu Simon Nabila Laroui Marianne Heyraud Guillaume Laconde Lamiaa M. A. Ali Kevin Bourbiaux Gilles Subra Lubomir L. Vezenkov Baptiste Legrand Muriel Amblard Nadir Bettache |
| author_facet | Matthieu Simon Nabila Laroui Marianne Heyraud Guillaume Laconde Lamiaa M. A. Ali Kevin Bourbiaux Gilles Subra Lubomir L. Vezenkov Baptiste Legrand Muriel Amblard Nadir Bettache |
| author_sort | Matthieu Simon |
| collection | DOAJ |
| description | Small interfering RNAs (siRNAs) are promising molecules for developing new therapies based on gene silencing; however, their delivery into cells remains an issue. In this study, we took advantage of stapled peptide technology that has emerged as a valuable strategy to render natural peptides more structured, resistant to protease degradation and more bioavailable, to develop short carriers for siRNA delivery. From the pool of stapled peptides that we have designed and synthesized, we identified non-toxic vectors that were able to efficiently encapsulate siRNA, transport them into the cell and induce gene silencing. Remarkably, the most efficient stapled peptide (JMV6582), is composed of only eight amino-acids and contains only two cationic charges. |
| first_indexed | 2024-03-10T14:35:07Z |
| format | Article |
| id | doaj.art-8b7e6fbf33b649009beeae6876cd91ae |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T14:35:07Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-8b7e6fbf33b649009beeae6876cd91ae2023-11-20T22:15:14ZengMDPI AGNanomaterials2079-49912020-11-011012233410.3390/nano10122334Hydrocarbon-Stapled Peptide Based-Nanoparticles for siRNA DeliveryMatthieu Simon0Nabila Laroui1Marianne Heyraud2Guillaume Laconde3Lamiaa M. A. Ali4Kevin Bourbiaux5Gilles Subra6Lubomir L. Vezenkov7Baptiste Legrand8Muriel Amblard9Nadir Bettache10IBMM, Univ Montpellier, CNRS, ENSCM, 34093 Montpellier, FranceIBMM, Univ Montpellier, CNRS, ENSCM, 34093 Montpellier, FranceIBMM, Univ Montpellier, CNRS, ENSCM, 34093 Montpellier, FranceIBMM, Univ Montpellier, CNRS, ENSCM, 34093 Montpellier, FranceIBMM, Univ Montpellier, CNRS, ENSCM, 34093 Montpellier, FranceIBMM, Univ Montpellier, CNRS, ENSCM, 34093 Montpellier, FranceIBMM, Univ Montpellier, CNRS, ENSCM, 34093 Montpellier, FranceIBMM, Univ Montpellier, CNRS, ENSCM, 34093 Montpellier, FranceIBMM, Univ Montpellier, CNRS, ENSCM, 34093 Montpellier, FranceIBMM, Univ Montpellier, CNRS, ENSCM, 34093 Montpellier, FranceIBMM, Univ Montpellier, CNRS, ENSCM, 34093 Montpellier, FranceSmall interfering RNAs (siRNAs) are promising molecules for developing new therapies based on gene silencing; however, their delivery into cells remains an issue. In this study, we took advantage of stapled peptide technology that has emerged as a valuable strategy to render natural peptides more structured, resistant to protease degradation and more bioavailable, to develop short carriers for siRNA delivery. From the pool of stapled peptides that we have designed and synthesized, we identified non-toxic vectors that were able to efficiently encapsulate siRNA, transport them into the cell and induce gene silencing. Remarkably, the most efficient stapled peptide (JMV6582), is composed of only eight amino-acids and contains only two cationic charges.https://www.mdpi.com/2079-4991/10/12/2334CPPStapled peptidessiRNAgene silencing |
| spellingShingle | Matthieu Simon Nabila Laroui Marianne Heyraud Guillaume Laconde Lamiaa M. A. Ali Kevin Bourbiaux Gilles Subra Lubomir L. Vezenkov Baptiste Legrand Muriel Amblard Nadir Bettache Hydrocarbon-Stapled Peptide Based-Nanoparticles for siRNA Delivery Nanomaterials CPP Stapled peptides siRNA gene silencing |
| title | Hydrocarbon-Stapled Peptide Based-Nanoparticles for siRNA Delivery |
| title_full | Hydrocarbon-Stapled Peptide Based-Nanoparticles for siRNA Delivery |
| title_fullStr | Hydrocarbon-Stapled Peptide Based-Nanoparticles for siRNA Delivery |
| title_full_unstemmed | Hydrocarbon-Stapled Peptide Based-Nanoparticles for siRNA Delivery |
| title_short | Hydrocarbon-Stapled Peptide Based-Nanoparticles for siRNA Delivery |
| title_sort | hydrocarbon stapled peptide based nanoparticles for sirna delivery |
| topic | CPP Stapled peptides siRNA gene silencing |
| url | https://www.mdpi.com/2079-4991/10/12/2334 |
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