AVALIAÇÃO DA EFICÁCIA DE CRIOPROTETORES PERMEANTES E NÃO PERMEANTES NO DESCONGELAMENTO RÁPIDO E LENTO DO SÊMEN CANINO
The aim of this study was to evaluate the association between cryoprotectants little studied in Brazil such as dimethylformamide and trehalose amid thinner, using protocols of fast and slow defrosting. Three adult Labrador Retrievier male, healthy dogs, weekly submitted to one semen collection durin...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Universidade Federal de Goiás
2014-03-01
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| Series: | Ciência Animal Brasileira |
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| Online Access: | http://www.revistas.ufg.br/index.php/vet/article/view/26124/16234 |
| _version_ | 1818112149236482048 |
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| author | Amanda Carla Acipreste Eduardo Paulino Costa2 Fabrício Albani Oliveira Sanely Lourenço Costa Talita Fernandes Silva Daniel Cortes Beretta |
| author_facet | Amanda Carla Acipreste Eduardo Paulino Costa2 Fabrício Albani Oliveira Sanely Lourenço Costa Talita Fernandes Silva Daniel Cortes Beretta |
| author_sort | Amanda Carla Acipreste |
| collection | DOAJ |
| description | The aim of this study was to evaluate the association between cryoprotectants little studied in Brazil such as dimethylformamide and trehalose amid thinner, using protocols of fast and slow defrosting. Three adult Labrador Retrievier male, healthy dogs, weekly submitted to one semen collection during five-weeks period, were used. The base diluent medium used in this study was tris-citrate added with 3% of dimethylformamide + 3% glycerol (D1), 3% dimethylformamide and trehalose (D2) and 4% glycerol (D3). At defrosting, half of the semen samples from each diluent medium was defrosted by rapid method in water-bath at 75 °C for seven minutes, followed by a new immersion at 37 °C for 1 minute. The other half of the samples was defrosted by slow method, in water-bath at 37 °C for 1 minute. The semen was evaluated for sperm progressive motility and vigor, besides membrane integrity. For this, the semen samples were submitted to either hyposmotic and membrane integrity tests of the plasmatic membrane and acrosome (fluorescence). The results indicated that the use of glycerol as cryoprotector in TRIS diluter provides greater efficacy in cryopreserving spermatozoa of the canine species, when compared to dimethylformamide associated with trehalose or glycerol. |
| first_indexed | 2024-12-11T03:14:21Z |
| format | Article |
| id | doaj.art-8b7edfd7f9984050a92a075889e43b1c |
| institution | Directory Open Access Journal |
| issn | 1518-2797 1809-6891 |
| language | English |
| last_indexed | 2024-12-11T03:14:21Z |
| publishDate | 2014-03-01 |
| publisher | Universidade Federal de Goiás |
| record_format | Article |
| series | Ciência Animal Brasileira |
| spelling | doaj.art-8b7edfd7f9984050a92a075889e43b1c2022-12-22T01:22:47ZengUniversidade Federal de GoiásCiência Animal Brasileira1518-27971809-68912014-03-0115110711410.5216/cab.v15i1.26124AVALIAÇÃO DA EFICÁCIA DE CRIOPROTETORES PERMEANTES E NÃO PERMEANTES NO DESCONGELAMENTO RÁPIDO E LENTO DO SÊMEN CANINOAmanda Carla Acipreste0Eduardo Paulino Costa21Fabrício Albani Oliveira2Sanely Lourenço Costa3Talita Fernandes Silva4Daniel Cortes Beretta5Pós-Graduanda da Faculdade de Ciências Agrárias e Veterinárias da UNESP, Jaboticabal, SP, Brasil Professor Doutor da Universidade Federal de Viçosa Viçosa, MG, Brasil.Pós-Graduando da Universidade Federal de Viçosa Viçosa, MG, Brasil.Médica Veterinária, Mestre da Universidade Federal de Viçosa Viçosa, MG, Brasil. Pós-Graduanda da Universidade Federal de Viçosa Viçosa, MG, Brasil.Professor Doutor da Universidade de Rio Verde, Rio Verde GO, Brasil The aim of this study was to evaluate the association between cryoprotectants little studied in Brazil such as dimethylformamide and trehalose amid thinner, using protocols of fast and slow defrosting. Three adult Labrador Retrievier male, healthy dogs, weekly submitted to one semen collection during five-weeks period, were used. The base diluent medium used in this study was tris-citrate added with 3% of dimethylformamide + 3% glycerol (D1), 3% dimethylformamide and trehalose (D2) and 4% glycerol (D3). At defrosting, half of the semen samples from each diluent medium was defrosted by rapid method in water-bath at 75 °C for seven minutes, followed by a new immersion at 37 °C for 1 minute. The other half of the samples was defrosted by slow method, in water-bath at 37 °C for 1 minute. The semen was evaluated for sperm progressive motility and vigor, besides membrane integrity. For this, the semen samples were submitted to either hyposmotic and membrane integrity tests of the plasmatic membrane and acrosome (fluorescence). The results indicated that the use of glycerol as cryoprotector in TRIS diluter provides greater efficacy in cryopreserving spermatozoa of the canine species, when compared to dimethylformamide associated with trehalose or glycerol.http://www.revistas.ufg.br/index.php/vet/article/view/26124/16234cryopreservationdimethylformamidedogsglyceroltrehalose |
| spellingShingle | Amanda Carla Acipreste Eduardo Paulino Costa2 Fabrício Albani Oliveira Sanely Lourenço Costa Talita Fernandes Silva Daniel Cortes Beretta AVALIAÇÃO DA EFICÁCIA DE CRIOPROTETORES PERMEANTES E NÃO PERMEANTES NO DESCONGELAMENTO RÁPIDO E LENTO DO SÊMEN CANINO Ciência Animal Brasileira cryopreservation dimethylformamide dogs glycerol trehalose |
| title | AVALIAÇÃO DA EFICÁCIA DE CRIOPROTETORES PERMEANTES E NÃO PERMEANTES NO DESCONGELAMENTO RÁPIDO E LENTO DO SÊMEN CANINO |
| title_full | AVALIAÇÃO DA EFICÁCIA DE CRIOPROTETORES PERMEANTES E NÃO PERMEANTES NO DESCONGELAMENTO RÁPIDO E LENTO DO SÊMEN CANINO |
| title_fullStr | AVALIAÇÃO DA EFICÁCIA DE CRIOPROTETORES PERMEANTES E NÃO PERMEANTES NO DESCONGELAMENTO RÁPIDO E LENTO DO SÊMEN CANINO |
| title_full_unstemmed | AVALIAÇÃO DA EFICÁCIA DE CRIOPROTETORES PERMEANTES E NÃO PERMEANTES NO DESCONGELAMENTO RÁPIDO E LENTO DO SÊMEN CANINO |
| title_short | AVALIAÇÃO DA EFICÁCIA DE CRIOPROTETORES PERMEANTES E NÃO PERMEANTES NO DESCONGELAMENTO RÁPIDO E LENTO DO SÊMEN CANINO |
| title_sort | avaliacao da eficacia de crioprotetores permeantes e nao permeantes no descongelamento rapido e lento do semen canino |
| topic | cryopreservation dimethylformamide dogs glycerol trehalose |
| url | http://www.revistas.ufg.br/index.php/vet/article/view/26124/16234 |
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