In Vitro Cytotoxic Protective Effect of Alginate-Encapsulated Capsaicin Might Improve Skin Side Effects Associated with the Topical Application of Capsaicin
Chronic neuropathic pain, particularly peripheral pain, is a cause of great concern for diabetic patients. Current treatments include numerous agents such as capsaicinoids, a known deterrent of neuropathic pain despite the inconvenience associated with local side effects. In this context, the curren...
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| Format: | Article |
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MDPI AG
2021-03-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/5/1455 |
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| author | Ariana Hudita Bianca Galateanu Marieta Costache Carolina Negrei Rodica-Mariana Ion Lorena Iancu Octav Ginghina |
| author_facet | Ariana Hudita Bianca Galateanu Marieta Costache Carolina Negrei Rodica-Mariana Ion Lorena Iancu Octav Ginghina |
| author_sort | Ariana Hudita |
| collection | DOAJ |
| description | Chronic neuropathic pain, particularly peripheral pain, is a cause of great concern for diabetic patients. Current treatments include numerous agents such as capsaicinoids, a known deterrent of neuropathic pain despite the inconvenience associated with local side effects. In this context, the current work aims to elucidate the potential mechanisms involved in cytotoxicity by capsaicin and proposes an efficient formulation of capsaicin in alginate microcapsules, which significantly reduces side effects from capsaicin topical administration. For this, human dermal fibroblast cells were treated with alginate-microencapsulated capsaicin extracts and screened for potential cytotoxic effects produced by the treatment. Cell viability and morphology were examined, as well as oxidative stress status and anti-inflammatory potential. Our results show that the alginate encapsulated formulation of capsaicin exerted lower cytotoxic effects on human dermal fibroblasts as measured by cell viability and reactive oxygen species (ROS) production. Furthermore, the expression profiles of inflammatory cytokines were significantly altered by the treatment as compared with the control culture. |
| first_indexed | 2024-03-09T05:05:13Z |
| format | Article |
| id | doaj.art-8b81c498ec7d4b18b9d8d8d296e6cfed |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T05:05:13Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-8b81c498ec7d4b18b9d8d8d296e6cfed2023-12-03T12:56:11ZengMDPI AGMolecules1420-30492021-03-01265145510.3390/molecules26051455In Vitro Cytotoxic Protective Effect of Alginate-Encapsulated Capsaicin Might Improve Skin Side Effects Associated with the Topical Application of CapsaicinAriana Hudita0Bianca Galateanu1Marieta Costache2Carolina Negrei3Rodica-Mariana Ion4Lorena Iancu5Octav Ginghina6Department of Biochemistry and Molecular Biology, University of Bucharest, 050095 Bucharest, RomaniaDepartment of Biochemistry and Molecular Biology, University of Bucharest, 050095 Bucharest, RomaniaDepartment of Biochemistry and Molecular Biology, University of Bucharest, 050095 Bucharest, RomaniaDepartment of Toxicology, Faculty of Pharmacy, “Carol Davila” University of Medicine and Pharmacy, 020021 Bucharest, RomaniaNational Institute of Research and Development for Chemistry and Petrochemistry—ICECHIM, 060021 Bucharest, RomaniaNational Institute of Research and Development for Chemistry and Petrochemistry—ICECHIM, 060021 Bucharest, RomaniaDepartment of Surgery, “Sf. Ioan” Clinical Emergency Hospital, 042122 Bucharest, RomaniaChronic neuropathic pain, particularly peripheral pain, is a cause of great concern for diabetic patients. Current treatments include numerous agents such as capsaicinoids, a known deterrent of neuropathic pain despite the inconvenience associated with local side effects. In this context, the current work aims to elucidate the potential mechanisms involved in cytotoxicity by capsaicin and proposes an efficient formulation of capsaicin in alginate microcapsules, which significantly reduces side effects from capsaicin topical administration. For this, human dermal fibroblast cells were treated with alginate-microencapsulated capsaicin extracts and screened for potential cytotoxic effects produced by the treatment. Cell viability and morphology were examined, as well as oxidative stress status and anti-inflammatory potential. Our results show that the alginate encapsulated formulation of capsaicin exerted lower cytotoxic effects on human dermal fibroblasts as measured by cell viability and reactive oxygen species (ROS) production. Furthermore, the expression profiles of inflammatory cytokines were significantly altered by the treatment as compared with the control culture.https://www.mdpi.com/1420-3049/26/5/1455capsaicinalginate encapsulationneuropathic paincytotoxicityinflammation |
| spellingShingle | Ariana Hudita Bianca Galateanu Marieta Costache Carolina Negrei Rodica-Mariana Ion Lorena Iancu Octav Ginghina In Vitro Cytotoxic Protective Effect of Alginate-Encapsulated Capsaicin Might Improve Skin Side Effects Associated with the Topical Application of Capsaicin Molecules capsaicin alginate encapsulation neuropathic pain cytotoxicity inflammation |
| title | In Vitro Cytotoxic Protective Effect of Alginate-Encapsulated Capsaicin Might Improve Skin Side Effects Associated with the Topical Application of Capsaicin |
| title_full | In Vitro Cytotoxic Protective Effect of Alginate-Encapsulated Capsaicin Might Improve Skin Side Effects Associated with the Topical Application of Capsaicin |
| title_fullStr | In Vitro Cytotoxic Protective Effect of Alginate-Encapsulated Capsaicin Might Improve Skin Side Effects Associated with the Topical Application of Capsaicin |
| title_full_unstemmed | In Vitro Cytotoxic Protective Effect of Alginate-Encapsulated Capsaicin Might Improve Skin Side Effects Associated with the Topical Application of Capsaicin |
| title_short | In Vitro Cytotoxic Protective Effect of Alginate-Encapsulated Capsaicin Might Improve Skin Side Effects Associated with the Topical Application of Capsaicin |
| title_sort | in vitro cytotoxic protective effect of alginate encapsulated capsaicin might improve skin side effects associated with the topical application of capsaicin |
| topic | capsaicin alginate encapsulation neuropathic pain cytotoxicity inflammation |
| url | https://www.mdpi.com/1420-3049/26/5/1455 |
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