Berberine alleviates oxidative stress in rats with osteoporosis through receptor activator of NF-kB/receptor activator of NF-kB ligand/osteoprotegerin (RANK/RANKL/OPG) pathway
Previous studies suggested that oxidative stress is related to the onset and development of osteoporosis. Moreover, it was demonstrated that berberine has a protective effect against oxidative stress-induced injuries. In this study, we aimed to investigate the effect and mechanism of action of berb...
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| Format: | Article |
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Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina
2017-11-01
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| Series: | Biomolecules & Biomedicine |
| Subjects: | |
| Online Access: | https://www.bjbms.org/ojs/index.php/bjbms/article/view/2596 |
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| author | Xiao-Feng He Long Zhang Chun-Hua Zhang Con-Ran Zhao Heng Li Ling-Fei Zhang Guo-Feng Tian Ming-Feng Guo Zheng Dai Fu-Ge Sui |
| author_facet | Xiao-Feng He Long Zhang Chun-Hua Zhang Con-Ran Zhao Heng Li Ling-Fei Zhang Guo-Feng Tian Ming-Feng Guo Zheng Dai Fu-Ge Sui |
| author_sort | Xiao-Feng He |
| collection | DOAJ |
| description |
Previous studies suggested that oxidative stress is related to the onset and development of osteoporosis. Moreover, it was demonstrated that berberine has a protective effect against oxidative stress-induced injuries. In this study, we aimed to investigate the effect and mechanism of action of berberine on rats with induced osteoporosis. Sixty 8-week-old female Wistar rats were randomly divided into the following 6 groups: control saline-treated, osteoporosis saline-treated, 3 osteoporosis berberine-treated groups (Ber 5, 10, and 20 mg/kg/body weight, respectively), and osteoporosis alendronate-treated (ALD) group. Osteoporosis was induced by bilateral ovariectomy. All treatments were performed for 8 weeks. The bone mineral density (BMD), serum alkaline phosphatase (ALP), osteocalcin, calcium, phosphorus, superoxide dismutase (SOD), methylenedioxyamphetamine (MDA), and glutathione peroxidase (GSH-Px) level was determined in the rat femur tissue. The gene and protein expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kappa-B ligand (RANKL) was analyzed by quantitative reverse transcription PCR and Western blot, respectively. The BMD, SOD and GSH⁃Px levels, and the expression of OPG were significantly lower in osteoporosis compared to control group (all p < 0.05). The serum levels of osteocalcin, ALP, and MDA, and the expression of RANKL were significantly higher in osteoporosis compared to control group (all p < 0.05). Berberine, especially the high doses of berberine, effectively increased SOD, GSH⁃Px, and OPG levels as well as decreased serum osteocalcin, ALP, MDA and RANKL levels in berberine-treated osteoporosis groups (all p < 0.05). To conclude, oxidative stress may promote the development of osteoporosis in rats through the RANK/RANKL/OPG pathway. The antioxidative effect of berberine reduces the development of osteoporosis in rats to some extent.
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| first_indexed | 2024-04-24T23:32:33Z |
| format | Article |
| id | doaj.art-8b88d1600c0f495c944a17929d4c2a4e |
| institution | Directory Open Access Journal |
| issn | 2831-0896 2831-090X |
| language | English |
| last_indexed | 2024-04-24T23:32:33Z |
| publishDate | 2017-11-01 |
| publisher | Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina |
| record_format | Article |
| series | Biomolecules & Biomedicine |
| spelling | doaj.art-8b88d1600c0f495c944a17929d4c2a4e2024-03-15T14:31:04ZengAssociation of Basic Medical Sciences of Federation of Bosnia and HerzegovinaBiomolecules & Biomedicine2831-08962831-090X2017-11-0117410.17305/bjbms.2017.2596356Berberine alleviates oxidative stress in rats with osteoporosis through receptor activator of NF-kB/receptor activator of NF-kB ligand/osteoprotegerin (RANK/RANKL/OPG) pathwayXiao-Feng He0Long Zhang1Chun-Hua Zhang2Con-Ran Zhao3Heng Li4Ling-Fei Zhang5Guo-Feng Tian6Ming-Feng Guo7Zheng Dai8Fu-Ge Sui9Department of Orthopaedics, Daqing Longnan Hospital, Daqing city of Heilongjiang province, ChinaDepartment of Orthopaedics, General Hospital of Daqing Oil Field, Daqing city of Heilongjiang province, ChinaDepartment of Gastroenterology, General Hospital of Daqing Oil Field, Daqing city of Heilongjiang province, ChinaDepartment of Orthopaedics, Daqing Longnan Hospital, Daqing city of Heilongjiang province, ChinaDepartment of Orthopaedics, Daqing Longnan Hospital, Daqing city of Heilongjiang province, ChinaDepartment of Orthopaedics, Daqing Longnan Hospital, Daqing city of Heilongjiang province, ChinaDepartment of Orthopaedics, Daqing Longnan Hospital, Daqing city of Heilongjiang province, ChinaDepartment of Orthopaedics, Daqing Longnan Hospital, Daqing city of Heilongjiang province, ChinaDepartment of Orthopaedics, Daqing Longnan Hospital, Daqing city of Heilongjiang province, ChinaDepartment of Orthopaedics, Daqing Longnan Hospital, Daqing city of Heilongjiang province, China Previous studies suggested that oxidative stress is related to the onset and development of osteoporosis. Moreover, it was demonstrated that berberine has a protective effect against oxidative stress-induced injuries. In this study, we aimed to investigate the effect and mechanism of action of berberine on rats with induced osteoporosis. Sixty 8-week-old female Wistar rats were randomly divided into the following 6 groups: control saline-treated, osteoporosis saline-treated, 3 osteoporosis berberine-treated groups (Ber 5, 10, and 20 mg/kg/body weight, respectively), and osteoporosis alendronate-treated (ALD) group. Osteoporosis was induced by bilateral ovariectomy. All treatments were performed for 8 weeks. The bone mineral density (BMD), serum alkaline phosphatase (ALP), osteocalcin, calcium, phosphorus, superoxide dismutase (SOD), methylenedioxyamphetamine (MDA), and glutathione peroxidase (GSH-Px) level was determined in the rat femur tissue. The gene and protein expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kappa-B ligand (RANKL) was analyzed by quantitative reverse transcription PCR and Western blot, respectively. The BMD, SOD and GSH⁃Px levels, and the expression of OPG were significantly lower in osteoporosis compared to control group (all p < 0.05). The serum levels of osteocalcin, ALP, and MDA, and the expression of RANKL were significantly higher in osteoporosis compared to control group (all p < 0.05). Berberine, especially the high doses of berberine, effectively increased SOD, GSH⁃Px, and OPG levels as well as decreased serum osteocalcin, ALP, MDA and RANKL levels in berberine-treated osteoporosis groups (all p < 0.05). To conclude, oxidative stress may promote the development of osteoporosis in rats through the RANK/RANKL/OPG pathway. The antioxidative effect of berberine reduces the development of osteoporosis in rats to some extent. https://www.bjbms.org/ojs/index.php/bjbms/article/view/2596Osteoporosisoxidative stressWistar ratsberberineOPGosteoprotegerin |
| spellingShingle | Xiao-Feng He Long Zhang Chun-Hua Zhang Con-Ran Zhao Heng Li Ling-Fei Zhang Guo-Feng Tian Ming-Feng Guo Zheng Dai Fu-Ge Sui Berberine alleviates oxidative stress in rats with osteoporosis through receptor activator of NF-kB/receptor activator of NF-kB ligand/osteoprotegerin (RANK/RANKL/OPG) pathway Biomolecules & Biomedicine Osteoporosis oxidative stress Wistar rats berberine OPG osteoprotegerin |
| title | Berberine alleviates oxidative stress in rats with osteoporosis through receptor activator of NF-kB/receptor activator of NF-kB ligand/osteoprotegerin (RANK/RANKL/OPG) pathway |
| title_full | Berberine alleviates oxidative stress in rats with osteoporosis through receptor activator of NF-kB/receptor activator of NF-kB ligand/osteoprotegerin (RANK/RANKL/OPG) pathway |
| title_fullStr | Berberine alleviates oxidative stress in rats with osteoporosis through receptor activator of NF-kB/receptor activator of NF-kB ligand/osteoprotegerin (RANK/RANKL/OPG) pathway |
| title_full_unstemmed | Berberine alleviates oxidative stress in rats with osteoporosis through receptor activator of NF-kB/receptor activator of NF-kB ligand/osteoprotegerin (RANK/RANKL/OPG) pathway |
| title_short | Berberine alleviates oxidative stress in rats with osteoporosis through receptor activator of NF-kB/receptor activator of NF-kB ligand/osteoprotegerin (RANK/RANKL/OPG) pathway |
| title_sort | berberine alleviates oxidative stress in rats with osteoporosis through receptor activator of nf kb receptor activator of nf kb ligand osteoprotegerin rank rankl opg pathway |
| topic | Osteoporosis oxidative stress Wistar rats berberine OPG osteoprotegerin |
| url | https://www.bjbms.org/ojs/index.php/bjbms/article/view/2596 |
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