Cloning and sequencing of the complicated rDNA gene family of Bos taurus
The rDNA genes coding for ribosomal RNA (rRNA) in animals are repeat sequences with high GC content and complicated structure. Based on the sequences of human ribosomal DNA repeat unit and transcription unit and the long and accurate PCR method with LA Taq DNA polymerase and GC buffer, we were able...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2006-10-01
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| Series: | Czech Journal of Animal Science |
| Subjects: | |
| Online Access: | https://cjas.agriculturejournals.cz/artkey/cjs-200610-0001_cloning-and-sequencing-of-the-complicated-rdna-gene-family-of-bos-taurus.php |
| _version_ | 1797899352950177792 |
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| author | D. Tang H. Jiang Y. Zhang Y. LI X. Zhang T. Zhou |
| author_facet | D. Tang H. Jiang Y. Zhang Y. LI X. Zhang T. Zhou |
| author_sort | D. Tang |
| collection | DOAJ |
| description | The rDNA genes coding for ribosomal RNA (rRNA) in animals are repeat sequences with high GC content and complicated structure. Based on the sequences of human ribosomal DNA repeat unit and transcription unit and the long and accurate PCR method with LA Taq DNA polymerase and GC buffer, we were able to amplify the complicated repeat sequences of rDNA genes in Bos taurus. Three rDNA genes and 2 internal transcribed spacer (ITS) fragments were cloned and confirmed by sequencing. The conditions for the cloning of complicated DNA sequences such as special rules of primer design, improvement of the reaction system, selection of DNA polymerase and adjustment of cycle parameters were discussed. |
| first_indexed | 2024-04-10T08:28:33Z |
| format | Article |
| id | doaj.art-8b8ae6ba16b8417baf757356663571ae |
| institution | Directory Open Access Journal |
| issn | 1212-1819 1805-9309 |
| language | English |
| last_indexed | 2024-04-10T08:28:33Z |
| publishDate | 2006-10-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Animal Science |
| spelling | doaj.art-8b8ae6ba16b8417baf757356663571ae2023-02-23T03:32:24ZengCzech Academy of Agricultural SciencesCzech Journal of Animal Science1212-18191805-93092006-10-01511042542810.17221/3960-CJAScjs-200610-0001Cloning and sequencing of the complicated rDNA gene family of Bos taurusD. Tang0H. Jiang1Y. Zhang2Y. LI3X. Zhang4T. Zhou5College of Life Science, Foshan University, Foshan, Guangdong, P.R. ChinaCollege of Life Science and Technology, Jinan University, Guangzhou, Guangdong, P.R. ChinaCollege of Life Science, Foshan University, Foshan, Guangdong, P.R. ChinaCollege of Life Science and Technology, Jinan University, Guangzhou, Guangdong, P.R. ChinaCollege of Life Science and Technology, Jinan University, Guangzhou, Guangdong, P.R. ChinaCollege of Life Science and Technology, Jinan University, Guangzhou, Guangdong, P.R. ChinaThe rDNA genes coding for ribosomal RNA (rRNA) in animals are repeat sequences with high GC content and complicated structure. Based on the sequences of human ribosomal DNA repeat unit and transcription unit and the long and accurate PCR method with LA Taq DNA polymerase and GC buffer, we were able to amplify the complicated repeat sequences of rDNA genes in Bos taurus. Three rDNA genes and 2 internal transcribed spacer (ITS) fragments were cloned and confirmed by sequencing. The conditions for the cloning of complicated DNA sequences such as special rules of primer design, improvement of the reaction system, selection of DNA polymerase and adjustment of cycle parameters were discussed.https://cjas.agriculturejournals.cz/artkey/cjs-200610-0001_cloning-and-sequencing-of-the-complicated-rdna-gene-family-of-bos-taurus.phpgc-richrepetitive sequencespcrrdnarrnabos taurus |
| spellingShingle | D. Tang H. Jiang Y. Zhang Y. LI X. Zhang T. Zhou Cloning and sequencing of the complicated rDNA gene family of Bos taurus Czech Journal of Animal Science gc-rich repetitive sequences pcr rdna rrna bos taurus |
| title | Cloning and sequencing of the complicated rDNA gene family of Bos taurus |
| title_full | Cloning and sequencing of the complicated rDNA gene family of Bos taurus |
| title_fullStr | Cloning and sequencing of the complicated rDNA gene family of Bos taurus |
| title_full_unstemmed | Cloning and sequencing of the complicated rDNA gene family of Bos taurus |
| title_short | Cloning and sequencing of the complicated rDNA gene family of Bos taurus |
| title_sort | cloning and sequencing of the complicated rdna gene family of bos taurus |
| topic | gc-rich repetitive sequences pcr rdna rrna bos taurus |
| url | https://cjas.agriculturejournals.cz/artkey/cjs-200610-0001_cloning-and-sequencing-of-the-complicated-rdna-gene-family-of-bos-taurus.php |
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