Feasibility of filamentous fungi for biofuel production using hydrolysate from dilute sulfuric acid pretreatment of wheat straw
<p>Abstract</p> <p>Background</p> <p>Lipids produced from filamentous fungi show great promise for biofuel production, but a major limiting factor is the high production cost attributed to feedstock. Lignocellulosic biomass is a suitable feedstock for biofuel production...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-07-01
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| Series: | Biotechnology for Biofuels |
| Subjects: | |
| Online Access: | http://www.biotechnologyforbiofuels.com/content/5/1/50 |
| _version_ | 1818543394681520128 |
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| author | Zheng Yubin Yu Xiaochen Zeng Jijiao Chen Shulin |
| author_facet | Zheng Yubin Yu Xiaochen Zeng Jijiao Chen Shulin |
| author_sort | Zheng Yubin |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Lipids produced from filamentous fungi show great promise for biofuel production, but a major limiting factor is the high production cost attributed to feedstock. Lignocellulosic biomass is a suitable feedstock for biofuel production due to its abundance and low value. However, very limited study has been performed on lipid production by culturing oleaginous fungi with lignocellulosic materials. Thus, identification of filamentous fungal strains capable of utilizing lignocellulosic hydrolysates for lipid accumulation is critical to improve the process and reduce the production cost.</p> <p>Results</p> <p>The growth performances of eleven filamentous fungi were investigated when cultured on glucose and xylose. Their dry cell weights, lipid contents and fatty acid profiles were determined. Six fungal strains with high lipid contents were selected to culture with the hydrolysate from dilute sulfuric acid pretreatment of wheat straw. The results showed that all the selected fungal strains were able to grow on both detoxified liquid hydrolysate (DLH) and non-detoxified liquid hydrolysate (NDLH). The highest lipid content of 39.4% was obtained by <it>Mortierella isabellina</it> on NDLH. In addition, NDLH with some precipitate could help <it>M. isabellina</it> form pellets with an average diameter of 0.11 mm.</p> <p>Conclusion</p> <p>This study demonstrated the possibility of fungal lipid production from lignocellulosic biomass. <it>M. isabellina</it> was the best lipid producer grown on lignocellulosic hydrolysates among the tested filamentous fungi, because it could not only accumulate oils with a high content by directly utilizing NDLH to simplify the fermentation process, but also form proper pellets to benefit the downstream harvesting. Considering the yield and cost, fungal lipids from lignocellulosic biomass are promising alternative sources for biodiesel production.</p> |
| first_indexed | 2024-12-11T22:34:49Z |
| format | Article |
| id | doaj.art-8b8b571dc8694eb4937eaeeefd9411af |
| institution | Directory Open Access Journal |
| issn | 1754-6834 |
| language | English |
| last_indexed | 2024-12-11T22:34:49Z |
| publishDate | 2012-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels |
| spelling | doaj.art-8b8b571dc8694eb4937eaeeefd9411af2022-12-22T00:48:00ZengBMCBiotechnology for Biofuels1754-68342012-07-01515010.1186/1754-6834-5-50Feasibility of filamentous fungi for biofuel production using hydrolysate from dilute sulfuric acid pretreatment of wheat strawZheng YubinYu XiaochenZeng JijiaoChen Shulin<p>Abstract</p> <p>Background</p> <p>Lipids produced from filamentous fungi show great promise for biofuel production, but a major limiting factor is the high production cost attributed to feedstock. Lignocellulosic biomass is a suitable feedstock for biofuel production due to its abundance and low value. However, very limited study has been performed on lipid production by culturing oleaginous fungi with lignocellulosic materials. Thus, identification of filamentous fungal strains capable of utilizing lignocellulosic hydrolysates for lipid accumulation is critical to improve the process and reduce the production cost.</p> <p>Results</p> <p>The growth performances of eleven filamentous fungi were investigated when cultured on glucose and xylose. Their dry cell weights, lipid contents and fatty acid profiles were determined. Six fungal strains with high lipid contents were selected to culture with the hydrolysate from dilute sulfuric acid pretreatment of wheat straw. The results showed that all the selected fungal strains were able to grow on both detoxified liquid hydrolysate (DLH) and non-detoxified liquid hydrolysate (NDLH). The highest lipid content of 39.4% was obtained by <it>Mortierella isabellina</it> on NDLH. In addition, NDLH with some precipitate could help <it>M. isabellina</it> form pellets with an average diameter of 0.11 mm.</p> <p>Conclusion</p> <p>This study demonstrated the possibility of fungal lipid production from lignocellulosic biomass. <it>M. isabellina</it> was the best lipid producer grown on lignocellulosic hydrolysates among the tested filamentous fungi, because it could not only accumulate oils with a high content by directly utilizing NDLH to simplify the fermentation process, but also form proper pellets to benefit the downstream harvesting. Considering the yield and cost, fungal lipids from lignocellulosic biomass are promising alternative sources for biodiesel production.</p>http://www.biotechnologyforbiofuels.com/content/5/1/50Filamentous fungi<it>Mortierella isabellina</it>Microbial lipidBiodieselLignocellulosic biomassWheat straw |
| spellingShingle | Zheng Yubin Yu Xiaochen Zeng Jijiao Chen Shulin Feasibility of filamentous fungi for biofuel production using hydrolysate from dilute sulfuric acid pretreatment of wheat straw Biotechnology for Biofuels Filamentous fungi <it>Mortierella isabellina</it> Microbial lipid Biodiesel Lignocellulosic biomass Wheat straw |
| title | Feasibility of filamentous fungi for biofuel production using hydrolysate from dilute sulfuric acid pretreatment of wheat straw |
| title_full | Feasibility of filamentous fungi for biofuel production using hydrolysate from dilute sulfuric acid pretreatment of wheat straw |
| title_fullStr | Feasibility of filamentous fungi for biofuel production using hydrolysate from dilute sulfuric acid pretreatment of wheat straw |
| title_full_unstemmed | Feasibility of filamentous fungi for biofuel production using hydrolysate from dilute sulfuric acid pretreatment of wheat straw |
| title_short | Feasibility of filamentous fungi for biofuel production using hydrolysate from dilute sulfuric acid pretreatment of wheat straw |
| title_sort | feasibility of filamentous fungi for biofuel production using hydrolysate from dilute sulfuric acid pretreatment of wheat straw |
| topic | Filamentous fungi <it>Mortierella isabellina</it> Microbial lipid Biodiesel Lignocellulosic biomass Wheat straw |
| url | http://www.biotechnologyforbiofuels.com/content/5/1/50 |
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