WDHD1 is over‐expressed in nasopharyngeal carcinoma and may control the expression of ITGAV
Nasopharyngeal carcinoma (NPC) is a highly metastatic and invasive malignant tumor that originates in the nasopharynx. The DNA‐binding protein WD repeat and HMG‐box DNA‐binding protein 1 (WDHD1) are highly expressed in a variety of tumours, but its expression and mechanism of action in NPC have not...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-01-01
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| Series: | FEBS Open Bio |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/2211-5463.13519 |
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| author | Ji‐Yun Wu Yi‐Tong Niu Su‐Ning Huang Yu‐Min Tan Zhen‐Dong Yang Ye‐Ying Fang Li Jiang Ting‐Ting Zhang Xiao‐Fen Zeng Yun‐Xi Peng Miao Mo Cai‐Xing Lin Zhu‐Xin Wei |
| author_facet | Ji‐Yun Wu Yi‐Tong Niu Su‐Ning Huang Yu‐Min Tan Zhen‐Dong Yang Ye‐Ying Fang Li Jiang Ting‐Ting Zhang Xiao‐Fen Zeng Yun‐Xi Peng Miao Mo Cai‐Xing Lin Zhu‐Xin Wei |
| author_sort | Ji‐Yun Wu |
| collection | DOAJ |
| description | Nasopharyngeal carcinoma (NPC) is a highly metastatic and invasive malignant tumor that originates in the nasopharynx. The DNA‐binding protein WD repeat and HMG‐box DNA‐binding protein 1 (WDHD1) are highly expressed in a variety of tumours, but its expression and mechanism of action in NPC have not been reported to date. To investigate the involvement of WDHD1 in NPC, we first mined databases for the gene expression profile of NPC. Immunohistochemistry (IHC) was performed on 338 cases of NPC and 112 non‐NPC samples to verify the results. We report that the expression of WDHD1 is significantly elevated in NPC. ChIP‐seq was used to show that integrin alpha V (ITGAV) and WDHD1 exhibit a significant binding peak in the promoter region of the ITGAV gene. The expression levels of ITGAV and WDHD1 exhibit a significant positive correlation, and IHC was performed to show that ITGAV is highly expressed in NPC. Expression of ITGAV increased after overexpression of WDHD1, suggesting that ITGAV may be a potential target gene of WDHD1. Pathway analysis showed that both genes were closely related to the cell cycle, and flow cytometry was used to further confirm that decreased expression of WDHD1 significantly increased the number of apoptotic cells. In conclusion, our results suggest that expression of WDHD1 is increased in NPC and is likely to be associated with the NPC cell cycle; thus, we propose that WDHD1 may have the potential as a target gene for primary screening and treatment of NPC. |
| first_indexed | 2024-04-11T01:31:36Z |
| format | Article |
| id | doaj.art-8b8da360520043ed909e1373fe75ade1 |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-04-11T01:31:36Z |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-8b8da360520043ed909e1373fe75ade12023-01-03T09:41:00ZengWileyFEBS Open Bio2211-54632023-01-0113110211710.1002/2211-5463.13519WDHD1 is over‐expressed in nasopharyngeal carcinoma and may control the expression of ITGAVJi‐Yun Wu0Yi‐Tong Niu1Su‐Ning Huang2Yu‐Min Tan3Zhen‐Dong Yang4Ye‐Ying Fang5Li Jiang6Ting‐Ting Zhang7Xiao‐Fen Zeng8Yun‐Xi Peng9Miao Mo10Cai‐Xing Lin11Zhu‐Xin Wei12Department of Radiotherapy First Affiliated Hospital of Guangxi Medical University Nanning ChinaDepartment of Radiotherapy First Affiliated Hospital of Guangxi Medical University Nanning ChinaDepartment of Radiotherapy Guangxi Medical University Cancer Hospital Nanning ChinaDepartment of Otolaryngology First People's Hospital of Hechi City Yizhou ChinaDepartment of Radiotherapy First Affiliated Hospital of Guangxi Medical University Nanning ChinaDepartment of Radiotherapy First Affiliated Hospital of Guangxi Medical University Nanning ChinaDepartment of Radiotherapy First Affiliated Hospital of Guangxi Medical University Nanning ChinaDepartment of Radiotherapy First Affiliated Hospital of Guangxi Medical University Nanning ChinaDepartment of Radiotherapy First Affiliated Hospital of Guangxi Medical University Nanning ChinaDepartment of Radiotherapy First Affiliated Hospital of Guangxi Medical University Nanning ChinaDepartment of Radiotherapy First Affiliated Hospital of Guangxi Medical University Nanning ChinaDepartment of Radiotherapy First Affiliated Hospital of Guangxi Medical University Nanning ChinaDepartment of Radiotherapy First Affiliated Hospital of Guangxi Medical University Nanning ChinaNasopharyngeal carcinoma (NPC) is a highly metastatic and invasive malignant tumor that originates in the nasopharynx. The DNA‐binding protein WD repeat and HMG‐box DNA‐binding protein 1 (WDHD1) are highly expressed in a variety of tumours, but its expression and mechanism of action in NPC have not been reported to date. To investigate the involvement of WDHD1 in NPC, we first mined databases for the gene expression profile of NPC. Immunohistochemistry (IHC) was performed on 338 cases of NPC and 112 non‐NPC samples to verify the results. We report that the expression of WDHD1 is significantly elevated in NPC. ChIP‐seq was used to show that integrin alpha V (ITGAV) and WDHD1 exhibit a significant binding peak in the promoter region of the ITGAV gene. The expression levels of ITGAV and WDHD1 exhibit a significant positive correlation, and IHC was performed to show that ITGAV is highly expressed in NPC. Expression of ITGAV increased after overexpression of WDHD1, suggesting that ITGAV may be a potential target gene of WDHD1. Pathway analysis showed that both genes were closely related to the cell cycle, and flow cytometry was used to further confirm that decreased expression of WDHD1 significantly increased the number of apoptotic cells. In conclusion, our results suggest that expression of WDHD1 is increased in NPC and is likely to be associated with the NPC cell cycle; thus, we propose that WDHD1 may have the potential as a target gene for primary screening and treatment of NPC.https://doi.org/10.1002/2211-5463.13519bioinformaticscell cycleimmunohistochemistrynasopharyngeal carcinomaWD repeat and HMG‐box DNA binding protein 1 |
| spellingShingle | Ji‐Yun Wu Yi‐Tong Niu Su‐Ning Huang Yu‐Min Tan Zhen‐Dong Yang Ye‐Ying Fang Li Jiang Ting‐Ting Zhang Xiao‐Fen Zeng Yun‐Xi Peng Miao Mo Cai‐Xing Lin Zhu‐Xin Wei WDHD1 is over‐expressed in nasopharyngeal carcinoma and may control the expression of ITGAV FEBS Open Bio bioinformatics cell cycle immunohistochemistry nasopharyngeal carcinoma WD repeat and HMG‐box DNA binding protein 1 |
| title | WDHD1 is over‐expressed in nasopharyngeal carcinoma and may control the expression of ITGAV |
| title_full | WDHD1 is over‐expressed in nasopharyngeal carcinoma and may control the expression of ITGAV |
| title_fullStr | WDHD1 is over‐expressed in nasopharyngeal carcinoma and may control the expression of ITGAV |
| title_full_unstemmed | WDHD1 is over‐expressed in nasopharyngeal carcinoma and may control the expression of ITGAV |
| title_short | WDHD1 is over‐expressed in nasopharyngeal carcinoma and may control the expression of ITGAV |
| title_sort | wdhd1 is over expressed in nasopharyngeal carcinoma and may control the expression of itgav |
| topic | bioinformatics cell cycle immunohistochemistry nasopharyngeal carcinoma WD repeat and HMG‐box DNA binding protein 1 |
| url | https://doi.org/10.1002/2211-5463.13519 |
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