Long non-coding HOXA-AS3 contributes to osteosarcoma progression through the miR-1286/TEAD1 axis
Abstract Long non-coding RNA (lncRNA) HOXA cluster antisense RNA 3 (HOXA-AS3) regulates the progression of several types of human malignancy. However, the role and potential mechanism of HOXA-AS3 in osteosarcoma (OS) remain unknown. In this study, upregulation of HOXA-AS3 was observed in OS tissues...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-09-01
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| Series: | Journal of Orthopaedic Surgery and Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13018-023-04214-5 |
| _version_ | 1797557730033008640 |
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| author | Xiangjun Xiao Mingjiang Liu Songlin Xie Changxiong Liu Xinfeng Huang Xiongjie Huang |
| author_facet | Xiangjun Xiao Mingjiang Liu Songlin Xie Changxiong Liu Xinfeng Huang Xiongjie Huang |
| author_sort | Xiangjun Xiao |
| collection | DOAJ |
| description | Abstract Long non-coding RNA (lncRNA) HOXA cluster antisense RNA 3 (HOXA-AS3) regulates the progression of several types of human malignancy. However, the role and potential mechanism of HOXA-AS3 in osteosarcoma (OS) remain unknown. In this study, upregulation of HOXA-AS3 was observed in OS tissues and cell lines and associated with poor clinical outcomes. Silencing of HOXA-AS3 significantly inhibited the proliferation, migration and invasion of OS cells in vitro and suppressed the tumorigenesis of OS cells in vivo. Furthermore, knockdown of HOXA-AS3 inhibited the proliferation and migration of human umbilical vein endothelial cells (HUVECs) and epithelial-to-mesenchymal transition (EMT) in OS. Further investigation of this mechanism revealed that HOXA-AS3 could directly upregulate the expression of TEAD1 via its competing endogenous RNA (ceRNA) activity on miR-1286. This study clarified the oncogenic roles of the HOXA-AS3/miR-1286/TEAD1 axis in OS progression, suggesting a novel therapeutic target for OS. |
| first_indexed | 2024-03-10T17:20:33Z |
| format | Article |
| id | doaj.art-8b8f0fd568464fd1be2c92a6a204992b |
| institution | Directory Open Access Journal |
| issn | 1749-799X |
| language | English |
| last_indexed | 2024-03-10T17:20:33Z |
| publishDate | 2023-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Orthopaedic Surgery and Research |
| spelling | doaj.art-8b8f0fd568464fd1be2c92a6a204992b2023-11-20T10:21:05ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2023-09-0118111410.1186/s13018-023-04214-5Long non-coding HOXA-AS3 contributes to osteosarcoma progression through the miR-1286/TEAD1 axisXiangjun Xiao0Mingjiang Liu1Songlin Xie2Changxiong Liu3Xinfeng Huang4Xiongjie Huang5Department of Hand and Foot Surgery, Nanhua Hospital Affiliated to Nanhua UniversityDepartment of Orthopedic Trauma and Hand Surgery, Changsha Central Hospital Affiliated to Nanhua UniversityDepartment of Hand and Foot Surgery, Nanhua Hospital Affiliated to Nanhua UniversityDepartment of Hand and Foot Surgery, Nanhua Hospital Affiliated to Nanhua UniversityDepartment of Hand and Foot Surgery, Nanhua Hospital Affiliated to Nanhua UniversityDepartment of Hand and Foot Surgery, Nanhua Hospital Affiliated to Nanhua UniversityAbstract Long non-coding RNA (lncRNA) HOXA cluster antisense RNA 3 (HOXA-AS3) regulates the progression of several types of human malignancy. However, the role and potential mechanism of HOXA-AS3 in osteosarcoma (OS) remain unknown. In this study, upregulation of HOXA-AS3 was observed in OS tissues and cell lines and associated with poor clinical outcomes. Silencing of HOXA-AS3 significantly inhibited the proliferation, migration and invasion of OS cells in vitro and suppressed the tumorigenesis of OS cells in vivo. Furthermore, knockdown of HOXA-AS3 inhibited the proliferation and migration of human umbilical vein endothelial cells (HUVECs) and epithelial-to-mesenchymal transition (EMT) in OS. Further investigation of this mechanism revealed that HOXA-AS3 could directly upregulate the expression of TEAD1 via its competing endogenous RNA (ceRNA) activity on miR-1286. This study clarified the oncogenic roles of the HOXA-AS3/miR-1286/TEAD1 axis in OS progression, suggesting a novel therapeutic target for OS.https://doi.org/10.1186/s13018-023-04214-5HOXA cluster antisense RNA 3MiR-1286OsteosarcomaTEA domain family mem |
| spellingShingle | Xiangjun Xiao Mingjiang Liu Songlin Xie Changxiong Liu Xinfeng Huang Xiongjie Huang Long non-coding HOXA-AS3 contributes to osteosarcoma progression through the miR-1286/TEAD1 axis Journal of Orthopaedic Surgery and Research HOXA cluster antisense RNA 3 MiR-1286 Osteosarcoma TEA domain family mem |
| title | Long non-coding HOXA-AS3 contributes to osteosarcoma progression through the miR-1286/TEAD1 axis |
| title_full | Long non-coding HOXA-AS3 contributes to osteosarcoma progression through the miR-1286/TEAD1 axis |
| title_fullStr | Long non-coding HOXA-AS3 contributes to osteosarcoma progression through the miR-1286/TEAD1 axis |
| title_full_unstemmed | Long non-coding HOXA-AS3 contributes to osteosarcoma progression through the miR-1286/TEAD1 axis |
| title_short | Long non-coding HOXA-AS3 contributes to osteosarcoma progression through the miR-1286/TEAD1 axis |
| title_sort | long non coding hoxa as3 contributes to osteosarcoma progression through the mir 1286 tead1 axis |
| topic | HOXA cluster antisense RNA 3 MiR-1286 Osteosarcoma TEA domain family mem |
| url | https://doi.org/10.1186/s13018-023-04214-5 |
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