In vitro and in vivo evaluation of liposomes modified with polypeptides and red cell membrane as a novel drug delivery system for myocardium targeting
Ischemic cardiac disease (ICD) is a cardiovascular disease with high morbidity and mortality. In this study, a novel myocardial targeted drug delivery system was developed represented by co-modified liposomes consisting of red cell membrane (RCM), and the peptides TAT and PCM. Liposomes were prepare...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Drug Delivery |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/10717544.2020.1754525 |
| _version_ | 1818740972832423936 |
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| author | Xueyan Liu Liangke Zhang Wengao Jiang Zhangyou Yang Zongjie Gan Chao Yu Ran Tao Huali Chen |
| author_facet | Xueyan Liu Liangke Zhang Wengao Jiang Zhangyou Yang Zongjie Gan Chao Yu Ran Tao Huali Chen |
| author_sort | Xueyan Liu |
| collection | DOAJ |
| description | Ischemic cardiac disease (ICD) is a cardiovascular disease with high morbidity and mortality. In this study, a novel myocardial targeted drug delivery system was developed represented by co-modified liposomes consisting of red cell membrane (RCM), and the peptides TAT and PCM. Liposomes were prepared using a membrane dispersion-ultrasonic method; the prepared 1% TAT and 3% PCM micelles were mixed with liposomes and under overnight stirring to form polypeptid-modified liposomes. RCM was isolated from mice blood, and the mechanical force facilitated RCM adhesion to the lipid bilayer. The characteristics of liposomes such as the morphology, particle size, zeta-potential, and RCM-conjugation to lipsomes were evaluated. Uptake efficiency and cellular toxicity of liposomes were evaluated in vitro on myocardial cells (MCs). As regard the experiments in vivo, liposomes were intravenously injected into mice, and the blood and organs were collectedat different times to analyze the pharmacokinetics profile of liposomes. The cellular uptake and intracellular distribution of liposomes of different composition into MCs demonstrated that RCM-modified liposomes had the best delivery capability. The pharmacokinetics study further demonstrated that RCM-modified liposomes had prolonged mean residence time (MRT) and more accumulation in the heart. This study indicated that RCM can be used to modify liposomes in combination with polypeptides, because such modification increases the myocardial targeting of liposomes. Therefore, this system constructed in this study might be a potentially effective myocardial drug delivery system. |
| first_indexed | 2024-12-18T01:49:13Z |
| format | Article |
| id | doaj.art-8b90f41c4e79438cbe4cc719cb4909c4 |
| institution | Directory Open Access Journal |
| issn | 1071-7544 1521-0464 |
| language | English |
| last_indexed | 2024-12-18T01:49:13Z |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Drug Delivery |
| spelling | doaj.art-8b90f41c4e79438cbe4cc719cb4909c42022-12-21T21:25:05ZengTaylor & Francis GroupDrug Delivery1071-75441521-04642020-01-0127159960610.1080/10717544.2020.17545251754525In vitro and in vivo evaluation of liposomes modified with polypeptides and red cell membrane as a novel drug delivery system for myocardium targetingXueyan Liu0Liangke Zhang1Wengao Jiang2Zhangyou Yang3Zongjie Gan4Chao Yu5Ran Tao6Huali Chen7College of Pharmacy, Chongqing Medical UniversityCollege of Pharmacy, Chongqing Medical UniversityCollege of Pharmacy, Chongqing Medical UniversityCollege of Pharmacy, Chongqing Medical UniversityCollege of Pharmacy, Chongqing Medical UniversityCollege of Pharmacy, Chongqing Medical UniversityCollege of Pharmacy, Chongqing Medical UniversityCollege of Pharmacy, Chongqing Medical UniversityIschemic cardiac disease (ICD) is a cardiovascular disease with high morbidity and mortality. In this study, a novel myocardial targeted drug delivery system was developed represented by co-modified liposomes consisting of red cell membrane (RCM), and the peptides TAT and PCM. Liposomes were prepared using a membrane dispersion-ultrasonic method; the prepared 1% TAT and 3% PCM micelles were mixed with liposomes and under overnight stirring to form polypeptid-modified liposomes. RCM was isolated from mice blood, and the mechanical force facilitated RCM adhesion to the lipid bilayer. The characteristics of liposomes such as the morphology, particle size, zeta-potential, and RCM-conjugation to lipsomes were evaluated. Uptake efficiency and cellular toxicity of liposomes were evaluated in vitro on myocardial cells (MCs). As regard the experiments in vivo, liposomes were intravenously injected into mice, and the blood and organs were collectedat different times to analyze the pharmacokinetics profile of liposomes. The cellular uptake and intracellular distribution of liposomes of different composition into MCs demonstrated that RCM-modified liposomes had the best delivery capability. The pharmacokinetics study further demonstrated that RCM-modified liposomes had prolonged mean residence time (MRT) and more accumulation in the heart. This study indicated that RCM can be used to modify liposomes in combination with polypeptides, because such modification increases the myocardial targeting of liposomes. Therefore, this system constructed in this study might be a potentially effective myocardial drug delivery system.http://dx.doi.org/10.1080/10717544.2020.1754525myocardium deliveryliposomestatpcmred cell membrane |
| spellingShingle | Xueyan Liu Liangke Zhang Wengao Jiang Zhangyou Yang Zongjie Gan Chao Yu Ran Tao Huali Chen In vitro and in vivo evaluation of liposomes modified with polypeptides and red cell membrane as a novel drug delivery system for myocardium targeting Drug Delivery myocardium delivery liposomes tat pcm red cell membrane |
| title | In vitro and in vivo evaluation of liposomes modified with polypeptides and red cell membrane as a novel drug delivery system for myocardium targeting |
| title_full | In vitro and in vivo evaluation of liposomes modified with polypeptides and red cell membrane as a novel drug delivery system for myocardium targeting |
| title_fullStr | In vitro and in vivo evaluation of liposomes modified with polypeptides and red cell membrane as a novel drug delivery system for myocardium targeting |
| title_full_unstemmed | In vitro and in vivo evaluation of liposomes modified with polypeptides and red cell membrane as a novel drug delivery system for myocardium targeting |
| title_short | In vitro and in vivo evaluation of liposomes modified with polypeptides and red cell membrane as a novel drug delivery system for myocardium targeting |
| title_sort | in vitro and in vivo evaluation of liposomes modified with polypeptides and red cell membrane as a novel drug delivery system for myocardium targeting |
| topic | myocardium delivery liposomes tat pcm red cell membrane |
| url | http://dx.doi.org/10.1080/10717544.2020.1754525 |
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