Evaluation of the derivative spectrophotometric technique for the quantification of ofloxacin and ciprofloxacin hydrochloride in their bulk drugs and pharmaceutical dosage forms
It was developed and validated as a sensitive, economical, and repeatable derivative visible-spectrophotometric method for determining ciprofloxacin hydrochloride and ofloxacin. The first, second, third, and fourth derivative spectra of the standard solutions were obtained by instrumental electronic...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-07-01
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| Series: | Results in Optics |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666950123001153 |
| _version_ | 1797733840124379136 |
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| author | Khaled Mansour Elgendy Monier Zaky Saad Alaa Eldin Turky Alsayed Fadel Osman |
| author_facet | Khaled Mansour Elgendy Monier Zaky Saad Alaa Eldin Turky Alsayed Fadel Osman |
| author_sort | Khaled Mansour Elgendy |
| collection | DOAJ |
| description | It was developed and validated as a sensitive, economical, and repeatable derivative visible-spectrophotometric method for determining ciprofloxacin hydrochloride and ofloxacin. The first, second, third, and fourth derivative spectra of the standard solutions were obtained by instrumental electronic differentiation with a delta lambda (Δλ) of 8 nm, and the spectra of the solutions were recorded in the range of 250–350 nm. The distances between the center zero baselines and the 280 nm negative peak were used to measure the analytical responses. The developed method achieves a low detection limit of 0.3 μg/L with a linear range of 2–20 μg/L. with a good precision (RSD ≤ 2.0%) The developed method is sensitive and precise. simple, accurate, and cost-effective, and exhibited a good correlation coefficient (R2 = 0.9998) and excellent mean recovery (99.20–100.08%), indicating a high degree of precision of the methods. The method's accuracy, precision, selectivity, robustness, and linearity were all demonstrated via a rigorous verification process in compliance with ICH guidelines. The recommended method may be used to control the quality of dosages that contain ofloxacien and ciprofloxacin hydrochloride as an active component since it is easy, inexpensive, and reliable enough. |
| first_indexed | 2024-03-12T12:35:24Z |
| format | Article |
| id | doaj.art-8b98de1610aa46cf8f13162d179f5eb2 |
| institution | Directory Open Access Journal |
| issn | 2666-9501 |
| language | English |
| last_indexed | 2024-03-12T12:35:24Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Results in Optics |
| spelling | doaj.art-8b98de1610aa46cf8f13162d179f5eb22023-08-29T04:18:11ZengElsevierResults in Optics2666-95012023-07-0112100463Evaluation of the derivative spectrophotometric technique for the quantification of ofloxacin and ciprofloxacin hydrochloride in their bulk drugs and pharmaceutical dosage formsKhaled Mansour Elgendy0Monier Zaky Saad1Alaa Eldin Turky2Alsayed Fadel Osman3Chemistry Department, Faculty of Science, Zagzig University, Zagzig, EgyptChemistry Department, Faculty of Science, Zagzig University, Zagzig, EgyptChemistry Department, Faculty of Science, Zagzig University, Zagzig, EgyptCorresponding author.; Chemistry Department, Faculty of Science, Zagzig University, Zagzig, EgyptIt was developed and validated as a sensitive, economical, and repeatable derivative visible-spectrophotometric method for determining ciprofloxacin hydrochloride and ofloxacin. The first, second, third, and fourth derivative spectra of the standard solutions were obtained by instrumental electronic differentiation with a delta lambda (Δλ) of 8 nm, and the spectra of the solutions were recorded in the range of 250–350 nm. The distances between the center zero baselines and the 280 nm negative peak were used to measure the analytical responses. The developed method achieves a low detection limit of 0.3 μg/L with a linear range of 2–20 μg/L. with a good precision (RSD ≤ 2.0%) The developed method is sensitive and precise. simple, accurate, and cost-effective, and exhibited a good correlation coefficient (R2 = 0.9998) and excellent mean recovery (99.20–100.08%), indicating a high degree of precision of the methods. The method's accuracy, precision, selectivity, robustness, and linearity were all demonstrated via a rigorous verification process in compliance with ICH guidelines. The recommended method may be used to control the quality of dosages that contain ofloxacien and ciprofloxacin hydrochloride as an active component since it is easy, inexpensive, and reliable enough.http://www.sciencedirect.com/science/article/pii/S2666950123001153Ciprofloxacin hydrochlorideOfloxacinDerivative spectrophotometryPharmaceutical analysisQuinolones |
| spellingShingle | Khaled Mansour Elgendy Monier Zaky Saad Alaa Eldin Turky Alsayed Fadel Osman Evaluation of the derivative spectrophotometric technique for the quantification of ofloxacin and ciprofloxacin hydrochloride in their bulk drugs and pharmaceutical dosage forms Results in Optics Ciprofloxacin hydrochloride Ofloxacin Derivative spectrophotometry Pharmaceutical analysis Quinolones |
| title | Evaluation of the derivative spectrophotometric technique for the quantification of ofloxacin and ciprofloxacin hydrochloride in their bulk drugs and pharmaceutical dosage forms |
| title_full | Evaluation of the derivative spectrophotometric technique for the quantification of ofloxacin and ciprofloxacin hydrochloride in their bulk drugs and pharmaceutical dosage forms |
| title_fullStr | Evaluation of the derivative spectrophotometric technique for the quantification of ofloxacin and ciprofloxacin hydrochloride in their bulk drugs and pharmaceutical dosage forms |
| title_full_unstemmed | Evaluation of the derivative spectrophotometric technique for the quantification of ofloxacin and ciprofloxacin hydrochloride in their bulk drugs and pharmaceutical dosage forms |
| title_short | Evaluation of the derivative spectrophotometric technique for the quantification of ofloxacin and ciprofloxacin hydrochloride in their bulk drugs and pharmaceutical dosage forms |
| title_sort | evaluation of the derivative spectrophotometric technique for the quantification of ofloxacin and ciprofloxacin hydrochloride in their bulk drugs and pharmaceutical dosage forms |
| topic | Ciprofloxacin hydrochloride Ofloxacin Derivative spectrophotometry Pharmaceutical analysis Quinolones |
| url | http://www.sciencedirect.com/science/article/pii/S2666950123001153 |
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