Novel Nematocidal Compounds from Shrimp Shell Wastes Valorized by <i>Bacillus velezensis</i> RB.EK7 against Black Pepper Nematodes
Among various organic wastes, shrimp shell powder (SSP) was the most suitable carbon/nitrogen source for producing antinematode compounds (ANCs) via <i>Bacillus veleznesis</i> RB.EK7 fermentation. The fermentation process for the enhancement of antinematode activity was investigated. <...
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MDPI AG
2022-09-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/12/10/2300 |
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| author | Thi Huyen Trang Trinh San-Lang Wang Van Bon Nguyen Tu Quy Phan Manh Dung Doan Thi Phuong Hanh Tran Thi Huyen Nguyen Thi Anh Hong Le That Quang Ton Anh Dzung Nguyen |
| author_facet | Thi Huyen Trang Trinh San-Lang Wang Van Bon Nguyen Tu Quy Phan Manh Dung Doan Thi Phuong Hanh Tran Thi Huyen Nguyen Thi Anh Hong Le That Quang Ton Anh Dzung Nguyen |
| author_sort | Thi Huyen Trang Trinh |
| collection | DOAJ |
| description | Among various organic wastes, shrimp shell powder (SSP) was the most suitable carbon/nitrogen source for producing antinematode compounds (ANCs) via <i>Bacillus veleznesis</i> RB.EK7 fermentation. The fermentation process for the enhancement of antinematode activity was investigated. <i>B. veleznesis</i> RB.EK7 produced the highest antinematode activity in the medium containing 0.8% SSP with an initial pH of 6.5–7.0, and fermentation was performed at 35–37 °C with a saking speed of 150 rpm for 72 h. Targeting ANCs were purified from the fermented culture broth and identified as thymine (<b>1</b>) and hexahydropyrrolo [1,2-a]pyrazine-1,4-dione (<b>2</b>) based on nuclear magnetic resonance (NMR) and mass spectra analysis and were compared to those of the reported compounds. Notably, for the first time, these compounds were found as novel ANCs. Thymine (<b>1</b>) demonstrated a potential nematicidal effect with near 100% mortality of second-stage juvenile (<i>J2</i>) nematodes and anti-egg hatching effects of 70.1%, while hexahydropyrrolo [1,2-a]pyrazine-1,4-dione showed moderate antinematode activities with 64.2% mortality of <i>J2</i> nematodes and anti-egg hatching effects of 57.9%. The docking study coupled with experimental enzyme inhibition results indicated that the potent nematicidal effect of these compounds may be possibly due to the inhibition of the targeting enzyme acetylcholinesterase. The data of this study suggest that SSP can be potentially reused for the eco-friendly production of ANCs for the management of black pepper nematodes. |
| first_indexed | 2024-03-09T20:54:37Z |
| format | Article |
| id | doaj.art-8b9b9b551d81420eb442c6f94188dc77 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-09T20:54:37Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-8b9b9b551d81420eb442c6f94188dc772023-11-23T22:24:42ZengMDPI AGAgronomy2073-43952022-09-011210230010.3390/agronomy12102300Novel Nematocidal Compounds from Shrimp Shell Wastes Valorized by <i>Bacillus velezensis</i> RB.EK7 against Black Pepper NematodesThi Huyen Trang Trinh0San-Lang Wang1Van Bon Nguyen2Tu Quy Phan3Manh Dung Doan4Thi Phuong Hanh Tran5Thi Huyen Nguyen6Thi Anh Hong Le7That Quang Ton8Anh Dzung Nguyen9Department of Science and Technology, Tay Nguyen University, Buon Ma Thuot 630000, VietnamDepartment of Chemistry, Tamkang University, New Taipei City 25137, TaiwanInstitute of Biotechnology and Environment, Tay Nguyen University, Buon Ma Thuot 630000, VietnamDepartment of Science and Technology, Tay Nguyen University, Buon Ma Thuot 630000, VietnamInstitute of Biotechnology and Environment, Tay Nguyen University, Buon Ma Thuot 630000, VietnamDepartment of Science and Technology, Tay Nguyen University, Buon Ma Thuot 630000, VietnamInstitute of Biotechnology and Environment, Tay Nguyen University, Buon Ma Thuot 630000, VietnamInstitute of Tropical Biology, Vietnam Academy of Science and Technology, Ho Chi Minh City 700000, VietnamFaculty of Chemistry, University of Science, Ho Chi Minh City 700000, VietnamInstitute of Biotechnology and Environment, Tay Nguyen University, Buon Ma Thuot 630000, VietnamAmong various organic wastes, shrimp shell powder (SSP) was the most suitable carbon/nitrogen source for producing antinematode compounds (ANCs) via <i>Bacillus veleznesis</i> RB.EK7 fermentation. The fermentation process for the enhancement of antinematode activity was investigated. <i>B. veleznesis</i> RB.EK7 produced the highest antinematode activity in the medium containing 0.8% SSP with an initial pH of 6.5–7.0, and fermentation was performed at 35–37 °C with a saking speed of 150 rpm for 72 h. Targeting ANCs were purified from the fermented culture broth and identified as thymine (<b>1</b>) and hexahydropyrrolo [1,2-a]pyrazine-1,4-dione (<b>2</b>) based on nuclear magnetic resonance (NMR) and mass spectra analysis and were compared to those of the reported compounds. Notably, for the first time, these compounds were found as novel ANCs. Thymine (<b>1</b>) demonstrated a potential nematicidal effect with near 100% mortality of second-stage juvenile (<i>J2</i>) nematodes and anti-egg hatching effects of 70.1%, while hexahydropyrrolo [1,2-a]pyrazine-1,4-dione showed moderate antinematode activities with 64.2% mortality of <i>J2</i> nematodes and anti-egg hatching effects of 57.9%. The docking study coupled with experimental enzyme inhibition results indicated that the potent nematicidal effect of these compounds may be possibly due to the inhibition of the targeting enzyme acetylcholinesterase. The data of this study suggest that SSP can be potentially reused for the eco-friendly production of ANCs for the management of black pepper nematodes.https://www.mdpi.com/2073-4395/12/10/2300black pepper<i>Bacillus veleznesis</i>nematodesantinematode compoundsorganic wastesmicrobial fermentation |
| spellingShingle | Thi Huyen Trang Trinh San-Lang Wang Van Bon Nguyen Tu Quy Phan Manh Dung Doan Thi Phuong Hanh Tran Thi Huyen Nguyen Thi Anh Hong Le That Quang Ton Anh Dzung Nguyen Novel Nematocidal Compounds from Shrimp Shell Wastes Valorized by <i>Bacillus velezensis</i> RB.EK7 against Black Pepper Nematodes Agronomy black pepper <i>Bacillus veleznesis</i> nematodes antinematode compounds organic wastes microbial fermentation |
| title | Novel Nematocidal Compounds from Shrimp Shell Wastes Valorized by <i>Bacillus velezensis</i> RB.EK7 against Black Pepper Nematodes |
| title_full | Novel Nematocidal Compounds from Shrimp Shell Wastes Valorized by <i>Bacillus velezensis</i> RB.EK7 against Black Pepper Nematodes |
| title_fullStr | Novel Nematocidal Compounds from Shrimp Shell Wastes Valorized by <i>Bacillus velezensis</i> RB.EK7 against Black Pepper Nematodes |
| title_full_unstemmed | Novel Nematocidal Compounds from Shrimp Shell Wastes Valorized by <i>Bacillus velezensis</i> RB.EK7 against Black Pepper Nematodes |
| title_short | Novel Nematocidal Compounds from Shrimp Shell Wastes Valorized by <i>Bacillus velezensis</i> RB.EK7 against Black Pepper Nematodes |
| title_sort | novel nematocidal compounds from shrimp shell wastes valorized by i bacillus velezensis i rb ek7 against black pepper nematodes |
| topic | black pepper <i>Bacillus veleznesis</i> nematodes antinematode compounds organic wastes microbial fermentation |
| url | https://www.mdpi.com/2073-4395/12/10/2300 |
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