Hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry method for quantification of five phospholipid classes in various matrices
Hydrophilic interaction liquid chromatography (HILIC), coupled to tandem mass spectrometry, can be used to separate and determine various polar lipid classes. The development of an HILIC chromatographic separation of several molecular species among five phospholipid classes (PC, PE, PG, PI and PS) i...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | MethodsX |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215016123000304 |
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| author | Nicolas Mazzella Aurélie Moreira Melissa Eon Arthur Médina Débora Millan-Navarro Nicolas Creusot |
| author_facet | Nicolas Mazzella Aurélie Moreira Melissa Eon Arthur Médina Débora Millan-Navarro Nicolas Creusot |
| author_sort | Nicolas Mazzella |
| collection | DOAJ |
| description | Hydrophilic interaction liquid chromatography (HILIC), coupled to tandem mass spectrometry, can be used to separate and determine various polar lipid classes. The development of an HILIC chromatographic separation of several molecular species among five phospholipid classes (PC, PE, PG, PI and PS) is reported here. In this method, a gradient with acetonitrile and 40 mM ammonium acetate buffer was employed. The initial composition was 95% of acetonitrile, then this proportion was decreased to 70% in order to elute all the compounds of interest for a total running time of 11 mins. Furthermore, mobile phase pH can affect the ionizable character of the compounds, according to their pKa values, and also the stationary phase charge state. The influence of such a parameter on both retention times and resolution was evaluated. Besides, the response of different kinds of internal standards (post-extraction standard addition) was evaluated in four different biological matrices, two microalgae extracts and two marine fish extracts. This study found that the recovery rates were between 70 and 140% of the expected value, with relative standard deviations between 10 and 35%, and then limited matrix effects. • HILIC approach can be used to separate phospholipid according to their polar head-group, and electrospray ionization in negative mode as well as MS/MS allows further identification of the molecular species within each phospholipid class. • Matrix effects are low and compensated with appropriate internal standards. • The limits of quantifications were ranging from 0.05 to 0.14 µg.mL−1, depending on the analyte. |
| first_indexed | 2024-03-13T03:33:32Z |
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| id | doaj.art-8b9cab14ca8e473ab9b454029a61dbc3 |
| institution | Directory Open Access Journal |
| issn | 2215-0161 |
| language | English |
| last_indexed | 2024-03-13T03:33:32Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | MethodsX |
| spelling | doaj.art-8b9cab14ca8e473ab9b454029a61dbc32023-06-24T05:17:03ZengElsevierMethodsX2215-01612023-01-0110102026Hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry method for quantification of five phospholipid classes in various matricesNicolas Mazzella0Aurélie Moreira1Melissa Eon2Arthur Médina3Débora Millan-Navarro4Nicolas Creusot5Corresponding author.; INRAE Nouvelle-Aquitaine Bordeaux, UR EABX, 50 avenue de Verdun, Cestas 33612, FranceINRAE Nouvelle-Aquitaine Bordeaux, UR EABX, 50 avenue de Verdun, Cestas 33612, FranceINRAE Nouvelle-Aquitaine Bordeaux, UR EABX, 50 avenue de Verdun, Cestas 33612, FranceINRAE Nouvelle-Aquitaine Bordeaux, UR EABX, 50 avenue de Verdun, Cestas 33612, FranceINRAE Nouvelle-Aquitaine Bordeaux, UR EABX, 50 avenue de Verdun, Cestas 33612, FranceINRAE Nouvelle-Aquitaine Bordeaux, UR EABX, 50 avenue de Verdun, Cestas 33612, FranceHydrophilic interaction liquid chromatography (HILIC), coupled to tandem mass spectrometry, can be used to separate and determine various polar lipid classes. The development of an HILIC chromatographic separation of several molecular species among five phospholipid classes (PC, PE, PG, PI and PS) is reported here. In this method, a gradient with acetonitrile and 40 mM ammonium acetate buffer was employed. The initial composition was 95% of acetonitrile, then this proportion was decreased to 70% in order to elute all the compounds of interest for a total running time of 11 mins. Furthermore, mobile phase pH can affect the ionizable character of the compounds, according to their pKa values, and also the stationary phase charge state. The influence of such a parameter on both retention times and resolution was evaluated. Besides, the response of different kinds of internal standards (post-extraction standard addition) was evaluated in four different biological matrices, two microalgae extracts and two marine fish extracts. This study found that the recovery rates were between 70 and 140% of the expected value, with relative standard deviations between 10 and 35%, and then limited matrix effects. • HILIC approach can be used to separate phospholipid according to their polar head-group, and electrospray ionization in negative mode as well as MS/MS allows further identification of the molecular species within each phospholipid class. • Matrix effects are low and compensated with appropriate internal standards. • The limits of quantifications were ranging from 0.05 to 0.14 µg.mL−1, depending on the analyte.http://www.sciencedirect.com/science/article/pii/S2215016123000304Hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry method for quantification of five phospholipid classes in various matrices |
| spellingShingle | Nicolas Mazzella Aurélie Moreira Melissa Eon Arthur Médina Débora Millan-Navarro Nicolas Creusot Hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry method for quantification of five phospholipid classes in various matrices MethodsX Hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry method for quantification of five phospholipid classes in various matrices |
| title | Hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry method for quantification of five phospholipid classes in various matrices |
| title_full | Hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry method for quantification of five phospholipid classes in various matrices |
| title_fullStr | Hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry method for quantification of five phospholipid classes in various matrices |
| title_full_unstemmed | Hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry method for quantification of five phospholipid classes in various matrices |
| title_short | Hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry method for quantification of five phospholipid classes in various matrices |
| title_sort | hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry method for quantification of five phospholipid classes in various matrices |
| topic | Hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry method for quantification of five phospholipid classes in various matrices |
| url | http://www.sciencedirect.com/science/article/pii/S2215016123000304 |
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