Competitiveness of Bradyrhizobium japonicum inoculation strain for soybean nodule occupancy
The competitiveness of Bradyrhizobium japonicum inoculation strain against indigenous rhizobia was examined in a soil pot experiment. The effect of inoculation strain was evaluated under different soil conditions: with or without previously grown soybean and applied commercial inoculant. Molecular i...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2022-01-01
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| Series: | Plant, Soil and Environment |
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| Online Access: | https://pse.agriculturejournals.cz/artkey/pse-202201-0006_competitiveness-of-bradyrhizobium-japonicum-inoculation-strain-for-soybean-nodule-occupancy.php |
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| author | Dragana Miljaković Jelena Marinković Maja Ignjatov Dragana Milošević Zorica Nikolić Branislava Tintor Vojin Đukić |
| author_facet | Dragana Miljaković Jelena Marinković Maja Ignjatov Dragana Milošević Zorica Nikolić Branislava Tintor Vojin Đukić |
| author_sort | Dragana Miljaković |
| collection | DOAJ |
| description | The competitiveness of Bradyrhizobium japonicum inoculation strain against indigenous rhizobia was examined in a soil pot experiment. The effect of inoculation strain was evaluated under different soil conditions: with or without previously grown soybean and applied commercial inoculant. Molecular identification of inoculation strain and investigated rhizobial isolates, obtained from nodules representing inoculated treatments, was performed based on 16S rDNA and enterobacterial repetitive intergenic consensus (ERIC) sequencing. Inoculation strain showed a significant effect on the investigated parameters in both soils. Higher nodule occupancy (45% vs. 18%), nodule number (111% vs. 5%), nodule dry weight (49% vs. 9%), shoot length (15% vs. 7%), root length (31% vs. 13%), shoot dry weight (34% vs. 11%), shoot nitrogen content (27% vs. 2%), and nodule nitrogen content (9% vs. 5%) was detected in soil without previously grown soybean and applied commercial inoculant. Soil had a significant effect on the shoot, root and nodule nitrogen content, while interaction of experimental factors significantly altered dry weight and nitrogen content of shoots, roots and nodules, as well as number of nodules. Nodulation parameters were significantly related with shoot dry weight, shoot and nodule nitrogen content. Symbiotic performance of inoculation strains in the field could be improved through co-selection for their competitiveness and effectiveness. |
| first_indexed | 2024-04-10T08:07:36Z |
| format | Article |
| id | doaj.art-8b9d2dc70af0497f8cc5aae624ce608d |
| institution | Directory Open Access Journal |
| issn | 1214-1178 1805-9368 |
| language | English |
| last_indexed | 2024-04-10T08:07:36Z |
| publishDate | 2022-01-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Plant, Soil and Environment |
| spelling | doaj.art-8b9d2dc70af0497f8cc5aae624ce608d2023-02-23T03:47:05ZengCzech Academy of Agricultural SciencesPlant, Soil and Environment1214-11781805-93682022-01-01681596410.17221/430/2021-PSEpse-202201-0006Competitiveness of Bradyrhizobium japonicum inoculation strain for soybean nodule occupancyDragana MiljakovićJelena Marinković0Maja Ignjatov1Dragana Milošević2Zorica Nikolić3Branislava Tintor4Vojin Đukić5Institute of Field and Vegetable Crops, Novi Sad, SerbiaInstitute of Field and Vegetable Crops, Novi Sad, SerbiaInstitute of Field and Vegetable Crops, Novi Sad, SerbiaInstitute of Field and Vegetable Crops, Novi Sad, SerbiaInstitute of Field and Vegetable Crops, Novi Sad, SerbiaInstitute of Field and Vegetable Crops, Novi Sad, SerbiaThe competitiveness of Bradyrhizobium japonicum inoculation strain against indigenous rhizobia was examined in a soil pot experiment. The effect of inoculation strain was evaluated under different soil conditions: with or without previously grown soybean and applied commercial inoculant. Molecular identification of inoculation strain and investigated rhizobial isolates, obtained from nodules representing inoculated treatments, was performed based on 16S rDNA and enterobacterial repetitive intergenic consensus (ERIC) sequencing. Inoculation strain showed a significant effect on the investigated parameters in both soils. Higher nodule occupancy (45% vs. 18%), nodule number (111% vs. 5%), nodule dry weight (49% vs. 9%), shoot length (15% vs. 7%), root length (31% vs. 13%), shoot dry weight (34% vs. 11%), shoot nitrogen content (27% vs. 2%), and nodule nitrogen content (9% vs. 5%) was detected in soil without previously grown soybean and applied commercial inoculant. Soil had a significant effect on the shoot, root and nodule nitrogen content, while interaction of experimental factors significantly altered dry weight and nitrogen content of shoots, roots and nodules, as well as number of nodules. Nodulation parameters were significantly related with shoot dry weight, shoot and nodule nitrogen content. Symbiotic performance of inoculation strains in the field could be improved through co-selection for their competitiveness and effectiveness.https://pse.agriculturejournals.cz/artkey/pse-202201-0006_competitiveness-of-bradyrhizobium-japonicum-inoculation-strain-for-soybean-nodule-occupancy.phpcompetitiveness for nodulationglycine maxnitrogen fixationprotein cropsymbiotic bacteria |
| spellingShingle | Dragana Miljaković Jelena Marinković Maja Ignjatov Dragana Milošević Zorica Nikolić Branislava Tintor Vojin Đukić Competitiveness of Bradyrhizobium japonicum inoculation strain for soybean nodule occupancy Plant, Soil and Environment competitiveness for nodulation glycine max nitrogen fixation protein crop symbiotic bacteria |
| title | Competitiveness of Bradyrhizobium japonicum inoculation strain for soybean nodule occupancy |
| title_full | Competitiveness of Bradyrhizobium japonicum inoculation strain for soybean nodule occupancy |
| title_fullStr | Competitiveness of Bradyrhizobium japonicum inoculation strain for soybean nodule occupancy |
| title_full_unstemmed | Competitiveness of Bradyrhizobium japonicum inoculation strain for soybean nodule occupancy |
| title_short | Competitiveness of Bradyrhizobium japonicum inoculation strain for soybean nodule occupancy |
| title_sort | competitiveness of bradyrhizobium japonicum inoculation strain for soybean nodule occupancy |
| topic | competitiveness for nodulation glycine max nitrogen fixation protein crop symbiotic bacteria |
| url | https://pse.agriculturejournals.cz/artkey/pse-202201-0006_competitiveness-of-bradyrhizobium-japonicum-inoculation-strain-for-soybean-nodule-occupancy.php |
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