Performance of urine cotinine and hypomethylation of AHRR and F2RL3 as biomarkers for smoking exposure in a population-based cohort.
There is a growing body of evidence demonstrating an association between smoking and DNA methylation. Accordingly, DNA methylation is now considered a promising biomarker of smoking exposure. We evaluated the relationship between methylation markers (AHRR and F2RL3) and urine cotinine as well as sel...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2017-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5409156?pdf=render |
| _version_ | 1819145938031083520 |
|---|---|
| author | Do-Hoon Lee Sang-Hyun Hwang Min Kyung Lim Jin-Kyoung Oh Da Young Song E Hwa Yun Eun Young Park |
| author_facet | Do-Hoon Lee Sang-Hyun Hwang Min Kyung Lim Jin-Kyoung Oh Da Young Song E Hwa Yun Eun Young Park |
| author_sort | Do-Hoon Lee |
| collection | DOAJ |
| description | There is a growing body of evidence demonstrating an association between smoking and DNA methylation. Accordingly, DNA methylation is now considered a promising biomarker of smoking exposure. We evaluated the relationship between methylation markers (AHRR and F2RL3) and urine cotinine as well as self-reported smoking status. DNA methylation levels of AHRR and F2RL3 in blood as well as urine cotinine were measured in 330 adults (46 to 87 years of age). Pyrosequencing was performed to measure DNA methylation of AHRR and F2RL3 associated with smoking exposure. The lung cancer risk associated with DNA methylation and urine cotinine was analyzed using logistic regression analysis. The AHRR and F2RL3 genes were significantly hypomethylated in current smokers compared to in individuals who have never smoked. An inverse relationship was observed between urine cotinine and methylation levels. Methylation of AHRR and F2RL3 distinguished current smokers from never-smokers with high accuracy. Logistic multivariate analysis showed that AHRR methylation is significantly associated with the risk of lung cancer (OR = 0.96, P = 0.011). Our study validated the smoking-associated DNA methylation markers reported in a Korean population-based cohort. In conclusion, DNA methylation of AHRR and F2RL3 provided accurate measures for smoking exposure. Methylation markers reflecting the long-term effect of smoking on the risk of lung cancer showed better performance in distinguishing former smokers from never-smokers. |
| first_indexed | 2024-12-22T13:05:58Z |
| format | Article |
| id | doaj.art-8ba36f4ba02f45bd8871a6f94c043ea4 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T13:05:58Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-8ba36f4ba02f45bd8871a6f94c043ea42022-12-21T18:24:52ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01124e017678310.1371/journal.pone.0176783Performance of urine cotinine and hypomethylation of AHRR and F2RL3 as biomarkers for smoking exposure in a population-based cohort.Do-Hoon LeeSang-Hyun HwangMin Kyung LimJin-Kyoung OhDa Young SongE Hwa YunEun Young ParkThere is a growing body of evidence demonstrating an association between smoking and DNA methylation. Accordingly, DNA methylation is now considered a promising biomarker of smoking exposure. We evaluated the relationship between methylation markers (AHRR and F2RL3) and urine cotinine as well as self-reported smoking status. DNA methylation levels of AHRR and F2RL3 in blood as well as urine cotinine were measured in 330 adults (46 to 87 years of age). Pyrosequencing was performed to measure DNA methylation of AHRR and F2RL3 associated with smoking exposure. The lung cancer risk associated with DNA methylation and urine cotinine was analyzed using logistic regression analysis. The AHRR and F2RL3 genes were significantly hypomethylated in current smokers compared to in individuals who have never smoked. An inverse relationship was observed between urine cotinine and methylation levels. Methylation of AHRR and F2RL3 distinguished current smokers from never-smokers with high accuracy. Logistic multivariate analysis showed that AHRR methylation is significantly associated with the risk of lung cancer (OR = 0.96, P = 0.011). Our study validated the smoking-associated DNA methylation markers reported in a Korean population-based cohort. In conclusion, DNA methylation of AHRR and F2RL3 provided accurate measures for smoking exposure. Methylation markers reflecting the long-term effect of smoking on the risk of lung cancer showed better performance in distinguishing former smokers from never-smokers.http://europepmc.org/articles/PMC5409156?pdf=render |
| spellingShingle | Do-Hoon Lee Sang-Hyun Hwang Min Kyung Lim Jin-Kyoung Oh Da Young Song E Hwa Yun Eun Young Park Performance of urine cotinine and hypomethylation of AHRR and F2RL3 as biomarkers for smoking exposure in a population-based cohort. PLoS ONE |
| title | Performance of urine cotinine and hypomethylation of AHRR and F2RL3 as biomarkers for smoking exposure in a population-based cohort. |
| title_full | Performance of urine cotinine and hypomethylation of AHRR and F2RL3 as biomarkers for smoking exposure in a population-based cohort. |
| title_fullStr | Performance of urine cotinine and hypomethylation of AHRR and F2RL3 as biomarkers for smoking exposure in a population-based cohort. |
| title_full_unstemmed | Performance of urine cotinine and hypomethylation of AHRR and F2RL3 as biomarkers for smoking exposure in a population-based cohort. |
| title_short | Performance of urine cotinine and hypomethylation of AHRR and F2RL3 as biomarkers for smoking exposure in a population-based cohort. |
| title_sort | performance of urine cotinine and hypomethylation of ahrr and f2rl3 as biomarkers for smoking exposure in a population based cohort |
| url | http://europepmc.org/articles/PMC5409156?pdf=render |
| work_keys_str_mv | AT dohoonlee performanceofurinecotinineandhypomethylationofahrrandf2rl3asbiomarkersforsmokingexposureinapopulationbasedcohort AT sanghyunhwang performanceofurinecotinineandhypomethylationofahrrandf2rl3asbiomarkersforsmokingexposureinapopulationbasedcohort AT minkyunglim performanceofurinecotinineandhypomethylationofahrrandf2rl3asbiomarkersforsmokingexposureinapopulationbasedcohort AT jinkyoungoh performanceofurinecotinineandhypomethylationofahrrandf2rl3asbiomarkersforsmokingexposureinapopulationbasedcohort AT dayoungsong performanceofurinecotinineandhypomethylationofahrrandf2rl3asbiomarkersforsmokingexposureinapopulationbasedcohort AT ehwayun performanceofurinecotinineandhypomethylationofahrrandf2rl3asbiomarkersforsmokingexposureinapopulationbasedcohort AT eunyoungpark performanceofurinecotinineandhypomethylationofahrrandf2rl3asbiomarkersforsmokingexposureinapopulationbasedcohort |