Adding value to strawberry agro-industrial by-products through ultraviolet A-induced biofortification of antioxidant and anti-inflammatory phenolic compounds
BackgroundThe revalorization of agro-industrial by-products by applying ultraviolet A (UVA) radiation to biofortify with phenolic compounds has been studied in recent times, showing improvements in the individual and total phenolic content and their bioactivity. Therefore, the main aim of this work...
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.1080147/full |
| _version_ | 1811178460082601984 |
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| author | Esteban Villamil-Galindo Esteban Villamil-Galindo Marilena Antunes-Ricardo Marilena Antunes-Ricardo Andrea Marcela Piagentini Daniel A. Jacobo-Velázquez Daniel A. Jacobo-Velázquez |
| author_facet | Esteban Villamil-Galindo Esteban Villamil-Galindo Marilena Antunes-Ricardo Marilena Antunes-Ricardo Andrea Marcela Piagentini Daniel A. Jacobo-Velázquez Daniel A. Jacobo-Velázquez |
| author_sort | Esteban Villamil-Galindo |
| collection | DOAJ |
| description | BackgroundThe revalorization of agro-industrial by-products by applying ultraviolet A (UVA) radiation to biofortify with phenolic compounds has been studied in recent times, showing improvements in the individual and total phenolic content and their bioactivity. Therefore, the main aim of this work was to optimize the biofortification process of phenolic compounds by UVA radiation to strawberry agro-industrial by-products (RF). Moreover, the effect of UVA radiation on the potential biological activity of the phenolics accumulated in RF due to the treatment was also determined.MethodsThe assays followed a factorial design with three variables at three levels: UVA dose (LOW, MEDIUM, and HIGH), storage temperature (5, 10, and 15°C), and storage time (0, 24, 48, and 72 h). At each experimental condition, phenylalanine ammonia-lyase (PAL) and polyphenol oxidase (PPO) enzymatic activities, total phenolic compound content (TPC), phenolics profile (TPCHPLC), and agrimoniin content (AGN) were evaluated; and the optimal UVA dose, storage time, and temperature were determined. In vitro bioaccessibility of the accumulated phenolic compound was studied on RF tissue treated with UVA at optimal process conditions. The digested extracts were tested for antiproliferative activity in colorectal cancer cells, cellular antioxidant capacity, and anti-inflammatory activity.ResultsThe results showed that applying UVA-HIGH (86.4 KJ/m2) treatment and storing the tissue for 46 h at 15°C increased PAL activity (260%), phenolic content (240%), and AGN (300%). The biofortification process improves the bioaccessibility of the main phenolic compound of RF by 9.8 to 25%. The digested optimum extract showed an IC50 for HT29 and Caco-2 cells of 2.73 and 5.43 μg/mL, respectively, and presented 60% cellular antioxidant capacity and 30% inhibition of NOX production.ConclusionThe RF treated with UVA is an excellent source of phenolic compounds; specifically, ellagitannins and the UVA radiation proved to be efficient in biofortify RF, significantly improving the phenolic compounds content and their bioactive properties with adequate bioaccessibility, adding value to the strawberry agro-industrial by-products. |
| first_indexed | 2024-04-11T06:18:40Z |
| format | Article |
| id | doaj.art-8ba7df593e1747dda76e6fa985d99cc0 |
| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-04-11T06:18:40Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Nutrition |
| spelling | doaj.art-8ba7df593e1747dda76e6fa985d99cc02022-12-22T04:40:57ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2022-12-01910.3389/fnut.2022.10801471080147Adding value to strawberry agro-industrial by-products through ultraviolet A-induced biofortification of antioxidant and anti-inflammatory phenolic compoundsEsteban Villamil-Galindo0Esteban Villamil-Galindo1Marilena Antunes-Ricardo2Marilena Antunes-Ricardo3Andrea Marcela Piagentini4Daniel A. Jacobo-Velázquez5Daniel A. Jacobo-Velázquez6Instituto de Tecnología de Alimentos, Facultad de Ingeniería Química, Universidad Nacional del Litoral, Santa Fe, ArgentinaConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Santa Fe, ArgentinaTecnológico de Monterrey, The Institute for Obesity Research, Monterrey, MexicoTecnológico de Monterrey, Escuela de Ingeniería y Ciencias, Monterrey, MexicoInstituto de Tecnología de Alimentos, Facultad de Ingeniería Química, Universidad Nacional del Litoral, Santa Fe, ArgentinaTecnológico de Monterrey, The Institute for Obesity Research, Zapopan, MexicoTecnológico de Monterrey, Escuela de Ingeniería y Ciencias, Zapopan, MexicoBackgroundThe revalorization of agro-industrial by-products by applying ultraviolet A (UVA) radiation to biofortify with phenolic compounds has been studied in recent times, showing improvements in the individual and total phenolic content and their bioactivity. Therefore, the main aim of this work was to optimize the biofortification process of phenolic compounds by UVA radiation to strawberry agro-industrial by-products (RF). Moreover, the effect of UVA radiation on the potential biological activity of the phenolics accumulated in RF due to the treatment was also determined.MethodsThe assays followed a factorial design with three variables at three levels: UVA dose (LOW, MEDIUM, and HIGH), storage temperature (5, 10, and 15°C), and storage time (0, 24, 48, and 72 h). At each experimental condition, phenylalanine ammonia-lyase (PAL) and polyphenol oxidase (PPO) enzymatic activities, total phenolic compound content (TPC), phenolics profile (TPCHPLC), and agrimoniin content (AGN) were evaluated; and the optimal UVA dose, storage time, and temperature were determined. In vitro bioaccessibility of the accumulated phenolic compound was studied on RF tissue treated with UVA at optimal process conditions. The digested extracts were tested for antiproliferative activity in colorectal cancer cells, cellular antioxidant capacity, and anti-inflammatory activity.ResultsThe results showed that applying UVA-HIGH (86.4 KJ/m2) treatment and storing the tissue for 46 h at 15°C increased PAL activity (260%), phenolic content (240%), and AGN (300%). The biofortification process improves the bioaccessibility of the main phenolic compound of RF by 9.8 to 25%. The digested optimum extract showed an IC50 for HT29 and Caco-2 cells of 2.73 and 5.43 μg/mL, respectively, and presented 60% cellular antioxidant capacity and 30% inhibition of NOX production.ConclusionThe RF treated with UVA is an excellent source of phenolic compounds; specifically, ellagitannins and the UVA radiation proved to be efficient in biofortify RF, significantly improving the phenolic compounds content and their bioactive properties with adequate bioaccessibility, adding value to the strawberry agro-industrial by-products.https://www.frontiersin.org/articles/10.3389/fnut.2022.1080147/fullbiofortificationrevalorizationUV radiationcircular economyellagitanninspostharvest abiotic stresses |
| spellingShingle | Esteban Villamil-Galindo Esteban Villamil-Galindo Marilena Antunes-Ricardo Marilena Antunes-Ricardo Andrea Marcela Piagentini Daniel A. Jacobo-Velázquez Daniel A. Jacobo-Velázquez Adding value to strawberry agro-industrial by-products through ultraviolet A-induced biofortification of antioxidant and anti-inflammatory phenolic compounds Frontiers in Nutrition biofortification revalorization UV radiation circular economy ellagitannins postharvest abiotic stresses |
| title | Adding value to strawberry agro-industrial by-products through ultraviolet A-induced biofortification of antioxidant and anti-inflammatory phenolic compounds |
| title_full | Adding value to strawberry agro-industrial by-products through ultraviolet A-induced biofortification of antioxidant and anti-inflammatory phenolic compounds |
| title_fullStr | Adding value to strawberry agro-industrial by-products through ultraviolet A-induced biofortification of antioxidant and anti-inflammatory phenolic compounds |
| title_full_unstemmed | Adding value to strawberry agro-industrial by-products through ultraviolet A-induced biofortification of antioxidant and anti-inflammatory phenolic compounds |
| title_short | Adding value to strawberry agro-industrial by-products through ultraviolet A-induced biofortification of antioxidant and anti-inflammatory phenolic compounds |
| title_sort | adding value to strawberry agro industrial by products through ultraviolet a induced biofortification of antioxidant and anti inflammatory phenolic compounds |
| topic | biofortification revalorization UV radiation circular economy ellagitannins postharvest abiotic stresses |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.1080147/full |
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