In Vitro Evaluation of the Activity of Terpenes and Cannabidiol against Human Coronavirus E229
The activity of a new, terpene-based formulation, code-named NT-VRL-1, against Human Coronavirus (HCoV) strain 229E was evaluated in human lung fibroblasts (MRC-5 cells), with and without the addition of cannabidiol (CBD). The main constituents in the terpene formulation used for the experiment were...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-03-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/11/4/290 |
| _version_ | 1797539693220331520 |
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| author | Lior Chatow Adi Nudel Iris Nesher David Hayo Hemo Perri Rozenberg Hanna Voropaev Ilan Winkler Ronnie Levy Zohar Kerem Zohara Yaniv Nadav Eyal |
| author_facet | Lior Chatow Adi Nudel Iris Nesher David Hayo Hemo Perri Rozenberg Hanna Voropaev Ilan Winkler Ronnie Levy Zohar Kerem Zohara Yaniv Nadav Eyal |
| author_sort | Lior Chatow |
| collection | DOAJ |
| description | The activity of a new, terpene-based formulation, code-named NT-VRL-1, against Human Coronavirus (HCoV) strain 229E was evaluated in human lung fibroblasts (MRC-5 cells), with and without the addition of cannabidiol (CBD). The main constituents in the terpene formulation used for the experiment were beta caryophyllene, eucalyptol, and citral. The tested formulation exhibited an antiviral effect when it was pre-incubated with the host cells prior to virus infection. The combination of NT-VRL-1 with CBD potentiated the antiviral effect better than the positive controls pyrazofurin and glycyrrhizin. There was a strong correlation between the quantitative results from a cell-viability assay and the cytopathic effect seen under the microscope after 72 h. To the best of our knowledge, this is the first report of activity of a combination of terpenes and CBD against a coronavirus. |
| first_indexed | 2024-03-10T12:48:30Z |
| format | Article |
| id | doaj.art-8baaa13a90ac42c2913d478988d24c31 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-10T12:48:30Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-8baaa13a90ac42c2913d478988d24c312023-11-21T13:14:59ZengMDPI AGLife2075-17292021-03-0111429010.3390/life11040290In Vitro Evaluation of the Activity of Terpenes and Cannabidiol against Human Coronavirus E229Lior Chatow0Adi Nudel1Iris Nesher2David Hayo Hemo3Perri Rozenberg4Hanna Voropaev5Ilan Winkler6Ronnie Levy7Zohar Kerem8Zohara Yaniv9Nadav Eyal10Eybna Technologies Ltd., Givat Hen 43905, IsraelEybna Technologies Ltd., Givat Hen 43905, IsraelEybna Technologies Ltd., Givat Hen 43905, IsraelEybna Technologies Ltd., Givat Hen 43905, IsraelEybna Technologies Ltd., Givat Hen 43905, IsraelEybna Technologies Ltd., Givat Hen 43905, IsraelEybna Technologies Ltd., Givat Hen 43905, IsraelEybna Technologies Ltd., Givat Hen 43905, IsraelEybna Technologies Ltd., Givat Hen 43905, IsraelEybna Technologies Ltd., Givat Hen 43905, IsraelEybna Technologies Ltd., Givat Hen 43905, IsraelThe activity of a new, terpene-based formulation, code-named NT-VRL-1, against Human Coronavirus (HCoV) strain 229E was evaluated in human lung fibroblasts (MRC-5 cells), with and without the addition of cannabidiol (CBD). The main constituents in the terpene formulation used for the experiment were beta caryophyllene, eucalyptol, and citral. The tested formulation exhibited an antiviral effect when it was pre-incubated with the host cells prior to virus infection. The combination of NT-VRL-1 with CBD potentiated the antiviral effect better than the positive controls pyrazofurin and glycyrrhizin. There was a strong correlation between the quantitative results from a cell-viability assay and the cytopathic effect seen under the microscope after 72 h. To the best of our knowledge, this is the first report of activity of a combination of terpenes and CBD against a coronavirus.https://www.mdpi.com/2075-1729/11/4/290antiviralHCoV-229EterpenesCBDcannabisCOVID-19 |
| spellingShingle | Lior Chatow Adi Nudel Iris Nesher David Hayo Hemo Perri Rozenberg Hanna Voropaev Ilan Winkler Ronnie Levy Zohar Kerem Zohara Yaniv Nadav Eyal In Vitro Evaluation of the Activity of Terpenes and Cannabidiol against Human Coronavirus E229 Life antiviral HCoV-229E terpenes CBD cannabis COVID-19 |
| title | In Vitro Evaluation of the Activity of Terpenes and Cannabidiol against Human Coronavirus E229 |
| title_full | In Vitro Evaluation of the Activity of Terpenes and Cannabidiol against Human Coronavirus E229 |
| title_fullStr | In Vitro Evaluation of the Activity of Terpenes and Cannabidiol against Human Coronavirus E229 |
| title_full_unstemmed | In Vitro Evaluation of the Activity of Terpenes and Cannabidiol against Human Coronavirus E229 |
| title_short | In Vitro Evaluation of the Activity of Terpenes and Cannabidiol against Human Coronavirus E229 |
| title_sort | in vitro evaluation of the activity of terpenes and cannabidiol against human coronavirus e229 |
| topic | antiviral HCoV-229E terpenes CBD cannabis COVID-19 |
| url | https://www.mdpi.com/2075-1729/11/4/290 |
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