Effects of resveratrol on macrophages after phagocytosis of Candida glabrata
Candida glabrata is believed to be the underlying cause of many human ailments, including oral, gastrointestinal, and vaginal disorders. C. glabrata-caused deep-seated infections, coupled with its resistance to antifungal drugs, may contribute to a high mortality rate. Resveratrol is a polyphenol an...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-11-01
|
| Series: | International Journal of Medical Microbiology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1438422123000176 |
| _version_ | 1797389558511304704 |
|---|---|
| author | Zong-Han Chen Meng Guan Wei-Jia Zhao |
| author_facet | Zong-Han Chen Meng Guan Wei-Jia Zhao |
| author_sort | Zong-Han Chen |
| collection | DOAJ |
| description | Candida glabrata is believed to be the underlying cause of many human ailments, including oral, gastrointestinal, and vaginal disorders. C. glabrata-caused deep-seated infections, coupled with its resistance to antifungal drugs, may contribute to a high mortality rate. Resveratrol is a polyphenol and can achieve better therapeutic effects when administered in combination with micafungin, but the underlying molecular mechanisms remain unknown. Here, we investigate the effects of varying doses of resveratrol on the proliferation, apoptosis, and activity of macrophages, which were co-cultured with micafungin-pretreated C. glabrata. Resveratrol can restore the decreased proliferative activity of macrophages caused by the phagocytosis of C. glabrata. Further investigations demonstrated that this restoration ability exhibited a dose-dependent manner, reaching the highest level at 200 µM of resveratrol. Resveratrol tended to be more effective in inhibiting macrophage apoptosis and reducing reactive oxygen species (ROS) levels with concentration increases. In addition, at medium concentrations, resveratrol may down-regulate the expression of most inflammatory cytokines, whereas at high concentrations, it started to exert pro-inflammatory functions by up-regulating their expressions. Macrophages may shift from an anti-inflammatory (M2) phenotype to an inflammatory (M1) phenotype by resveratrol at 200 µM, and from M1 to M2 at 400 µM. Our research shows that resveratrol with micafungin are effective in treating C. glabrata infections. The resveratrol-micafungin combination can reduce the production of ROS, and promote the proliferation, inhibit the apoptosis, and activate the polarization of macrophages in a dose-dependent manner. This study offers insights into how this combination works and may provide possible direction for further clinical application of the combination. |
| first_indexed | 2024-03-08T22:57:39Z |
| format | Article |
| id | doaj.art-8bacbbbf1f7a4e80b00a881f8e2e9445 |
| institution | Directory Open Access Journal |
| issn | 1438-4221 |
| language | English |
| last_indexed | 2024-03-08T22:57:39Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal of Medical Microbiology |
| spelling | doaj.art-8bacbbbf1f7a4e80b00a881f8e2e94452023-12-16T06:06:19ZengElsevierInternational Journal of Medical Microbiology1438-42212023-11-013136151589Effects of resveratrol on macrophages after phagocytosis of Candida glabrataZong-Han Chen0Meng Guan1Wei-Jia Zhao2Yunnan University of Chinese Medicine, Kunming 650500, Yunnan, ChinaOphthalmology Department, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan, ChinaDepartment of Dermatology and Venereology, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan, China; Corresponding author.Candida glabrata is believed to be the underlying cause of many human ailments, including oral, gastrointestinal, and vaginal disorders. C. glabrata-caused deep-seated infections, coupled with its resistance to antifungal drugs, may contribute to a high mortality rate. Resveratrol is a polyphenol and can achieve better therapeutic effects when administered in combination with micafungin, but the underlying molecular mechanisms remain unknown. Here, we investigate the effects of varying doses of resveratrol on the proliferation, apoptosis, and activity of macrophages, which were co-cultured with micafungin-pretreated C. glabrata. Resveratrol can restore the decreased proliferative activity of macrophages caused by the phagocytosis of C. glabrata. Further investigations demonstrated that this restoration ability exhibited a dose-dependent manner, reaching the highest level at 200 µM of resveratrol. Resveratrol tended to be more effective in inhibiting macrophage apoptosis and reducing reactive oxygen species (ROS) levels with concentration increases. In addition, at medium concentrations, resveratrol may down-regulate the expression of most inflammatory cytokines, whereas at high concentrations, it started to exert pro-inflammatory functions by up-regulating their expressions. Macrophages may shift from an anti-inflammatory (M2) phenotype to an inflammatory (M1) phenotype by resveratrol at 200 µM, and from M1 to M2 at 400 µM. Our research shows that resveratrol with micafungin are effective in treating C. glabrata infections. The resveratrol-micafungin combination can reduce the production of ROS, and promote the proliferation, inhibit the apoptosis, and activate the polarization of macrophages in a dose-dependent manner. This study offers insights into how this combination works and may provide possible direction for further clinical application of the combination.http://www.sciencedirect.com/science/article/pii/S1438422123000176ResveratrolMacrophageCandida glabrataMicafunginApoptosisInflammatory cytokine |
| spellingShingle | Zong-Han Chen Meng Guan Wei-Jia Zhao Effects of resveratrol on macrophages after phagocytosis of Candida glabrata International Journal of Medical Microbiology Resveratrol Macrophage Candida glabrata Micafungin Apoptosis Inflammatory cytokine |
| title | Effects of resveratrol on macrophages after phagocytosis of Candida glabrata |
| title_full | Effects of resveratrol on macrophages after phagocytosis of Candida glabrata |
| title_fullStr | Effects of resveratrol on macrophages after phagocytosis of Candida glabrata |
| title_full_unstemmed | Effects of resveratrol on macrophages after phagocytosis of Candida glabrata |
| title_short | Effects of resveratrol on macrophages after phagocytosis of Candida glabrata |
| title_sort | effects of resveratrol on macrophages after phagocytosis of candida glabrata |
| topic | Resveratrol Macrophage Candida glabrata Micafungin Apoptosis Inflammatory cytokine |
| url | http://www.sciencedirect.com/science/article/pii/S1438422123000176 |
| work_keys_str_mv | AT zonghanchen effectsofresveratrolonmacrophagesafterphagocytosisofcandidaglabrata AT mengguan effectsofresveratrolonmacrophagesafterphagocytosisofcandidaglabrata AT weijiazhao effectsofresveratrolonmacrophagesafterphagocytosisofcandidaglabrata |