A liquid biopsy signature of circulating exosome-derived mRNAs, miRNAs and lncRNAs predict therapeutic efficacy to neoadjuvant chemotherapy in patients with advanced gastric cancer
Abstract At present, there is no validated marker to identify the subpopulation of patients with advanced gastric cancer (AGC) who might benefit from neoadjuvant chemotherapy (NACT). In view of this clinical challenge, the identification of non-invasive biomarkers for efficacy prediction of NACT in...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2022-12-01
|
| Series: | Molecular Cancer |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12943-022-01684-9 |
| _version_ | 1797980523645108224 |
|---|---|
| author | Ting Guo Xiao-Huan Tang Xiang-Yu Gao Yuan Zhou Bo Jin Zi-Qian Deng Ying Hu Xiao-Fang Xing Zi-Yu Li Jia-Fu Ji |
| author_facet | Ting Guo Xiao-Huan Tang Xiang-Yu Gao Yuan Zhou Bo Jin Zi-Qian Deng Ying Hu Xiao-Fang Xing Zi-Yu Li Jia-Fu Ji |
| author_sort | Ting Guo |
| collection | DOAJ |
| description | Abstract At present, there is no validated marker to identify the subpopulation of patients with advanced gastric cancer (AGC) who might benefit from neoadjuvant chemotherapy (NACT). In view of this clinical challenge, the identification of non-invasive biomarkers for efficacy prediction of NACT in patients with AGC is imperative. Herein, we aimed to develop a non-invasive, liquid-biopsy-based assay by using an exosome-derived RNAs model based on multi-omics characteristics of RNAs. We firstly used a multi-omics strategy to characterize the mRNAs, microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) profiles of circulating exosome enriched fractions in responders to NACT paired with non-responders, using RNA sequencing. Finally, numerous miRNAs, mRNAs and lncRNAs were identified to be associated with the response to NACT in patients with AGC, and it was validated in an independent cohort with promising AUC values. Furthermore, we established a 6-exosome-RNA panel that could robustly identified responders from non-responders treated with fluorouracil-based neoadjuvant chemotherapy. |
| first_indexed | 2024-04-11T05:55:34Z |
| format | Article |
| id | doaj.art-8bb0251e79ea42508a64b5ff9aa9eedb |
| institution | Directory Open Access Journal |
| issn | 1476-4598 |
| language | English |
| last_indexed | 2024-04-11T05:55:34Z |
| publishDate | 2022-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Cancer |
| spelling | doaj.art-8bb0251e79ea42508a64b5ff9aa9eedb2022-12-22T04:41:54ZengBMCMolecular Cancer1476-45982022-12-012111610.1186/s12943-022-01684-9A liquid biopsy signature of circulating exosome-derived mRNAs, miRNAs and lncRNAs predict therapeutic efficacy to neoadjuvant chemotherapy in patients with advanced gastric cancerTing Guo0Xiao-Huan Tang1Xiang-Yu Gao2Yuan Zhou3Bo Jin4Zi-Qian Deng5Ying Hu6Xiao-Fang Xing7Zi-Yu Li8Jia-Fu Ji9Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Division of Gastrointestinal Cancer Translational Research Laboratory, Peking University Cancer Hospital & InstituteKey Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Division of Gastrointestinal Cancer Translational Research Laboratory, Peking University Cancer Hospital & InstituteKey Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Division of Gastrointestinal Cancer Translational Research Laboratory, Peking University Cancer Hospital & InstituteDepartment of Biomedical Informatics, School of Basic Medical Sciences, Peking UniversityDepartment of Clinical Laboratory, Peking University First HospitalKey Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Division of Gastrointestinal Cancer Translational Research Laboratory, Peking University Cancer Hospital & InstituteBiological Sample Bank, Peking University Cancer Hospital & InstituteKey Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Division of Gastrointestinal Cancer Translational Research Laboratory, Peking University Cancer Hospital & InstituteKey Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Division of Gastrointestinal Cancer Translational Research Laboratory, Peking University Cancer Hospital & InstituteKey Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Division of Gastrointestinal Cancer Translational Research Laboratory, Peking University Cancer Hospital & InstituteAbstract At present, there is no validated marker to identify the subpopulation of patients with advanced gastric cancer (AGC) who might benefit from neoadjuvant chemotherapy (NACT). In view of this clinical challenge, the identification of non-invasive biomarkers for efficacy prediction of NACT in patients with AGC is imperative. Herein, we aimed to develop a non-invasive, liquid-biopsy-based assay by using an exosome-derived RNAs model based on multi-omics characteristics of RNAs. We firstly used a multi-omics strategy to characterize the mRNAs, microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) profiles of circulating exosome enriched fractions in responders to NACT paired with non-responders, using RNA sequencing. Finally, numerous miRNAs, mRNAs and lncRNAs were identified to be associated with the response to NACT in patients with AGC, and it was validated in an independent cohort with promising AUC values. Furthermore, we established a 6-exosome-RNA panel that could robustly identified responders from non-responders treated with fluorouracil-based neoadjuvant chemotherapy.https://doi.org/10.1186/s12943-022-01684-9ExosomeMulti-omics characteristics of RNAsAdvanced gastric cancerNeoadjuvant chemotherapyBiomarker panel for efficacy prediction |
| spellingShingle | Ting Guo Xiao-Huan Tang Xiang-Yu Gao Yuan Zhou Bo Jin Zi-Qian Deng Ying Hu Xiao-Fang Xing Zi-Yu Li Jia-Fu Ji A liquid biopsy signature of circulating exosome-derived mRNAs, miRNAs and lncRNAs predict therapeutic efficacy to neoadjuvant chemotherapy in patients with advanced gastric cancer Molecular Cancer Exosome Multi-omics characteristics of RNAs Advanced gastric cancer Neoadjuvant chemotherapy Biomarker panel for efficacy prediction |
| title | A liquid biopsy signature of circulating exosome-derived mRNAs, miRNAs and lncRNAs predict therapeutic efficacy to neoadjuvant chemotherapy in patients with advanced gastric cancer |
| title_full | A liquid biopsy signature of circulating exosome-derived mRNAs, miRNAs and lncRNAs predict therapeutic efficacy to neoadjuvant chemotherapy in patients with advanced gastric cancer |
| title_fullStr | A liquid biopsy signature of circulating exosome-derived mRNAs, miRNAs and lncRNAs predict therapeutic efficacy to neoadjuvant chemotherapy in patients with advanced gastric cancer |
| title_full_unstemmed | A liquid biopsy signature of circulating exosome-derived mRNAs, miRNAs and lncRNAs predict therapeutic efficacy to neoadjuvant chemotherapy in patients with advanced gastric cancer |
| title_short | A liquid biopsy signature of circulating exosome-derived mRNAs, miRNAs and lncRNAs predict therapeutic efficacy to neoadjuvant chemotherapy in patients with advanced gastric cancer |
| title_sort | liquid biopsy signature of circulating exosome derived mrnas mirnas and lncrnas predict therapeutic efficacy to neoadjuvant chemotherapy in patients with advanced gastric cancer |
| topic | Exosome Multi-omics characteristics of RNAs Advanced gastric cancer Neoadjuvant chemotherapy Biomarker panel for efficacy prediction |
| url | https://doi.org/10.1186/s12943-022-01684-9 |
| work_keys_str_mv | AT tingguo aliquidbiopsysignatureofcirculatingexosomederivedmrnasmirnasandlncrnaspredicttherapeuticefficacytoneoadjuvantchemotherapyinpatientswithadvancedgastriccancer AT xiaohuantang aliquidbiopsysignatureofcirculatingexosomederivedmrnasmirnasandlncrnaspredicttherapeuticefficacytoneoadjuvantchemotherapyinpatientswithadvancedgastriccancer AT xiangyugao aliquidbiopsysignatureofcirculatingexosomederivedmrnasmirnasandlncrnaspredicttherapeuticefficacytoneoadjuvantchemotherapyinpatientswithadvancedgastriccancer AT yuanzhou aliquidbiopsysignatureofcirculatingexosomederivedmrnasmirnasandlncrnaspredicttherapeuticefficacytoneoadjuvantchemotherapyinpatientswithadvancedgastriccancer AT bojin aliquidbiopsysignatureofcirculatingexosomederivedmrnasmirnasandlncrnaspredicttherapeuticefficacytoneoadjuvantchemotherapyinpatientswithadvancedgastriccancer AT ziqiandeng aliquidbiopsysignatureofcirculatingexosomederivedmrnasmirnasandlncrnaspredicttherapeuticefficacytoneoadjuvantchemotherapyinpatientswithadvancedgastriccancer AT yinghu aliquidbiopsysignatureofcirculatingexosomederivedmrnasmirnasandlncrnaspredicttherapeuticefficacytoneoadjuvantchemotherapyinpatientswithadvancedgastriccancer AT xiaofangxing aliquidbiopsysignatureofcirculatingexosomederivedmrnasmirnasandlncrnaspredicttherapeuticefficacytoneoadjuvantchemotherapyinpatientswithadvancedgastriccancer AT ziyuli aliquidbiopsysignatureofcirculatingexosomederivedmrnasmirnasandlncrnaspredicttherapeuticefficacytoneoadjuvantchemotherapyinpatientswithadvancedgastriccancer AT jiafuji aliquidbiopsysignatureofcirculatingexosomederivedmrnasmirnasandlncrnaspredicttherapeuticefficacytoneoadjuvantchemotherapyinpatientswithadvancedgastriccancer AT tingguo liquidbiopsysignatureofcirculatingexosomederivedmrnasmirnasandlncrnaspredicttherapeuticefficacytoneoadjuvantchemotherapyinpatientswithadvancedgastriccancer AT xiaohuantang liquidbiopsysignatureofcirculatingexosomederivedmrnasmirnasandlncrnaspredicttherapeuticefficacytoneoadjuvantchemotherapyinpatientswithadvancedgastriccancer AT xiangyugao liquidbiopsysignatureofcirculatingexosomederivedmrnasmirnasandlncrnaspredicttherapeuticefficacytoneoadjuvantchemotherapyinpatientswithadvancedgastriccancer AT yuanzhou liquidbiopsysignatureofcirculatingexosomederivedmrnasmirnasandlncrnaspredicttherapeuticefficacytoneoadjuvantchemotherapyinpatientswithadvancedgastriccancer AT bojin liquidbiopsysignatureofcirculatingexosomederivedmrnasmirnasandlncrnaspredicttherapeuticefficacytoneoadjuvantchemotherapyinpatientswithadvancedgastriccancer AT ziqiandeng liquidbiopsysignatureofcirculatingexosomederivedmrnasmirnasandlncrnaspredicttherapeuticefficacytoneoadjuvantchemotherapyinpatientswithadvancedgastriccancer AT yinghu liquidbiopsysignatureofcirculatingexosomederivedmrnasmirnasandlncrnaspredicttherapeuticefficacytoneoadjuvantchemotherapyinpatientswithadvancedgastriccancer AT xiaofangxing liquidbiopsysignatureofcirculatingexosomederivedmrnasmirnasandlncrnaspredicttherapeuticefficacytoneoadjuvantchemotherapyinpatientswithadvancedgastriccancer AT ziyuli liquidbiopsysignatureofcirculatingexosomederivedmrnasmirnasandlncrnaspredicttherapeuticefficacytoneoadjuvantchemotherapyinpatientswithadvancedgastriccancer AT jiafuji liquidbiopsysignatureofcirculatingexosomederivedmrnasmirnasandlncrnaspredicttherapeuticefficacytoneoadjuvantchemotherapyinpatientswithadvancedgastriccancer |