Diagnostic and prognostic value of long non-coding RNA PROX1‑AS1 and miR-647 expression in gastric cancer
Introduction. Gastric cancer remains one of the most common cancers and has a high mortality rate worldwide. Epigenetic alternations of non-coding RNAs (ncRNAs), including microRNAs and long ncRNAs can contribute to its pathogenesis and progression, and could be potent diagnostic and prognostic biom...
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| Format: | Article |
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ABV-press
2022-12-01
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| Series: | Успехи молекулярной онкологии |
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| Online Access: | https://umo.abvpress.ru/jour/article/view/476 |
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| author | E. A. Vetchinkina A. I. Kalinkin E. B. Kuznetsova A. E. Kiseleva E. A. Alekseeva M. V. Nemtsova I. V. Bure |
| author_facet | E. A. Vetchinkina A. I. Kalinkin E. B. Kuznetsova A. E. Kiseleva E. A. Alekseeva M. V. Nemtsova I. V. Bure |
| author_sort | E. A. Vetchinkina |
| collection | DOAJ |
| description | Introduction. Gastric cancer remains one of the most common cancers and has a high mortality rate worldwide. Epigenetic alternations of non-coding RNAs (ncRNAs), including microRNAs and long ncRNAs can contribute to its pathogenesis and progression, and could be potent diagnostic and prognostic biomarkers.Aim. Estimation of PROX1‑AS1 and miR-647 expression in gastric cancer and investigation of its clinical significance. Materials and methods. Tumor and adjacent normal tissues (n = 62), and sectional normal tissue samples (n = 5) were included in the study. The expression of the ncRNAs was quantified by reverse transcription-polymerase chain reaction assay.Results. We have reviled the significant difference in the PROX1‑AS1 expression in tumor (p = 0.002) and non-tumor tissues (p <0.001) obtained from gastric cancer patients in comparison with sectional gastric tissues without pathology. Pearson correlation analysis confirmed a negative correlation between PROX1‑AS1 and miR-647 in gastric cancer both in tumor (р <0,001) and adjacent normal tissues (р <0.001). Besides, expression of PROX1‑AS1 and miR-647 was associated with the size and extent of the primary tumor.Conclusion. The obtained results allow to suggest a potential prognostic value of PROX1‑AS1 and miR-647 in gastric cancer. |
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| institution | Directory Open Access Journal |
| issn | 2313-805X 2413-3787 |
| language | Russian |
| last_indexed | 2025-03-14T08:56:00Z |
| publishDate | 2022-12-01 |
| publisher | ABV-press |
| record_format | Article |
| series | Успехи молекулярной онкологии |
| spelling | doaj.art-8bba5b332d164bb9a04d0337b716d6792025-03-02T12:45:08ZrusABV-pressУспехи молекулярной онкологии2313-805X2413-37872022-12-019410.17650/2313-805X-2022-9-4-50-60261Diagnostic and prognostic value of long non-coding RNA PROX1‑AS1 and miR-647 expression in gastric cancerE. A. Vetchinkina0A. I. Kalinkin1E. B. Kuznetsova2A. E. Kiseleva3E. A. Alekseeva4M. V. Nemtsova5I. V. Bure6I.M. Sechenov First Moscow State Medical University, Ministry of Health of RussiaN.P. Bochkov Medical and Genetic Research CenterI.M. Sechenov First Moscow State Medical University, Ministry of Health of Russia; N.P. Bochkov Medical and Genetic Research CenterI.M. Sechenov First Moscow State Medical University, Ministry of Health of RussiaI.M. Sechenov First Moscow State Medical University, Ministry of Health of Russia; N.P. Bochkov Medical and Genetic Research CenterI.M. Sechenov First Moscow State Medical University, Ministry of Health of Russia; N.P. Bochkov Medical and Genetic Research CenterI.M. Sechenov First Moscow State Medical University, Ministry of Health of RussiaIntroduction. Gastric cancer remains one of the most common cancers and has a high mortality rate worldwide. Epigenetic alternations of non-coding RNAs (ncRNAs), including microRNAs and long ncRNAs can contribute to its pathogenesis and progression, and could be potent diagnostic and prognostic biomarkers.Aim. Estimation of PROX1‑AS1 and miR-647 expression in gastric cancer and investigation of its clinical significance. Materials and methods. Tumor and adjacent normal tissues (n = 62), and sectional normal tissue samples (n = 5) were included in the study. The expression of the ncRNAs was quantified by reverse transcription-polymerase chain reaction assay.Results. We have reviled the significant difference in the PROX1‑AS1 expression in tumor (p = 0.002) and non-tumor tissues (p <0.001) obtained from gastric cancer patients in comparison with sectional gastric tissues without pathology. Pearson correlation analysis confirmed a negative correlation between PROX1‑AS1 and miR-647 in gastric cancer both in tumor (р <0,001) and adjacent normal tissues (р <0.001). Besides, expression of PROX1‑AS1 and miR-647 was associated with the size and extent of the primary tumor.Conclusion. The obtained results allow to suggest a potential prognostic value of PROX1‑AS1 and miR-647 in gastric cancer.https://umo.abvpress.ru/jour/article/view/476non-coding rnaslong noncoding rnamicrornagastric cancerepigeneticsbiomarker |
| spellingShingle | E. A. Vetchinkina A. I. Kalinkin E. B. Kuznetsova A. E. Kiseleva E. A. Alekseeva M. V. Nemtsova I. V. Bure Diagnostic and prognostic value of long non-coding RNA PROX1‑AS1 and miR-647 expression in gastric cancer Успехи молекулярной онкологии non-coding rnas long noncoding rna microrna gastric cancer epigenetics biomarker |
| title | Diagnostic and prognostic value of long non-coding RNA PROX1‑AS1 and miR-647 expression in gastric cancer |
| title_full | Diagnostic and prognostic value of long non-coding RNA PROX1‑AS1 and miR-647 expression in gastric cancer |
| title_fullStr | Diagnostic and prognostic value of long non-coding RNA PROX1‑AS1 and miR-647 expression in gastric cancer |
| title_full_unstemmed | Diagnostic and prognostic value of long non-coding RNA PROX1‑AS1 and miR-647 expression in gastric cancer |
| title_short | Diagnostic and prognostic value of long non-coding RNA PROX1‑AS1 and miR-647 expression in gastric cancer |
| title_sort | diagnostic and prognostic value of long non coding rna prox1 as1 and mir 647 expression in gastric cancer |
| topic | non-coding rnas long noncoding rna microrna gastric cancer epigenetics biomarker |
| url | https://umo.abvpress.ru/jour/article/view/476 |
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