Optimization of Microwave-Assisted Extraction of Antihepatotoxic Triterpenoid from Actinidia deliciosa Root and Its Comparison with Conventional Extraction Methods

A simple and rapid microwave-assisted extraction (MAE) procedure has been developed and optimized for extracting triterpenoids (TTP) from the Actinidia deliciosa root. Several variables that could potentially affect the extraction efficiency, namely extraction time (min), ethanol fraction (%), liqui...

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Main Authors: Junjun Guan, Xinpeng Bai, Aiyong Qiu
Format: Article
Language:English
Published: University of Zagreb Faculty of Food Technology and Biotechnology 2007-01-01
Series:Food Technology and Biotechnology
Subjects:
Online Access:http://hrcak.srce.hr/file/43769
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author Junjun Guan
Xinpeng Bai
Aiyong Qiu
author_facet Junjun Guan
Xinpeng Bai
Aiyong Qiu
author_sort Junjun Guan
collection DOAJ
description A simple and rapid microwave-assisted extraction (MAE) procedure has been developed and optimized for extracting triterpenoids (TTP) from the Actinidia deliciosa root. Several variables that could potentially affect the extraction efficiency, namely extraction time (min), ethanol fraction (%), liquid:solid ratio (volume per mass) and microwave power (W) have been optimized by means of response surface methodology (RSM). The results suggest that ethanol concentration and microwave power were statistically most significant factors. The optimal conditions were determined and three-dimensional response surfaces were plotted from the mathematical models. The optimum conditions were ethanol fraction 72.67 %, microwave power 362.12 W, liquid:solid ratio (volume per mass) 15:1 and extraction time 30 min. Under the optimum operating conditions, the percentage of extracted TTP was 84.96 %, and MAE showed significantly higher recoveries than those obtained by the conventional extraction methods (ultrasonic and reflux extraction). In addition, a drastic reduction of the extraction time (30 min vs. 8 h) and solvent consumption (15 vs. 30) was achieved, comparable with that provided by the reflux extraction as a reference method.
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spelling doaj.art-8bbaab1e1af64c6fa55a03ad3d5f54d52023-12-02T11:25:11ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062007-01-01452174180Optimization of Microwave-Assisted Extraction of Antihepatotoxic Triterpenoid from Actinidia deliciosa Root and Its Comparison with Conventional Extraction MethodsJunjun Guan0Xinpeng Bai1Aiyong Qiu2College of Bioengineering, Henan University of Technology, Zhengzhou 450052, PR ChinaCollege of Science and Engineering, South China University of Tropical Agriculture, Hainan 571737, PR ChinaThe Key Laboratory of Food Science and Safety, Ministry of Education, Southern Yangtze University, Wuxi 214036, PR ChinaA simple and rapid microwave-assisted extraction (MAE) procedure has been developed and optimized for extracting triterpenoids (TTP) from the Actinidia deliciosa root. Several variables that could potentially affect the extraction efficiency, namely extraction time (min), ethanol fraction (%), liquid:solid ratio (volume per mass) and microwave power (W) have been optimized by means of response surface methodology (RSM). The results suggest that ethanol concentration and microwave power were statistically most significant factors. The optimal conditions were determined and three-dimensional response surfaces were plotted from the mathematical models. The optimum conditions were ethanol fraction 72.67 %, microwave power 362.12 W, liquid:solid ratio (volume per mass) 15:1 and extraction time 30 min. Under the optimum operating conditions, the percentage of extracted TTP was 84.96 %, and MAE showed significantly higher recoveries than those obtained by the conventional extraction methods (ultrasonic and reflux extraction). In addition, a drastic reduction of the extraction time (30 min vs. 8 h) and solvent consumption (15 vs. 30) was achieved, comparable with that provided by the reflux extraction as a reference method.http://hrcak.srce.hr/file/43769microwave-assisted extraction (MAE)Actinidia deliciosa roottriterpenoidresponse surface methodology
spellingShingle Junjun Guan
Xinpeng Bai
Aiyong Qiu
Optimization of Microwave-Assisted Extraction of Antihepatotoxic Triterpenoid from Actinidia deliciosa Root and Its Comparison with Conventional Extraction Methods
Food Technology and Biotechnology
microwave-assisted extraction (MAE)
Actinidia deliciosa root
triterpenoid
response surface methodology
title Optimization of Microwave-Assisted Extraction of Antihepatotoxic Triterpenoid from Actinidia deliciosa Root and Its Comparison with Conventional Extraction Methods
title_full Optimization of Microwave-Assisted Extraction of Antihepatotoxic Triterpenoid from Actinidia deliciosa Root and Its Comparison with Conventional Extraction Methods
title_fullStr Optimization of Microwave-Assisted Extraction of Antihepatotoxic Triterpenoid from Actinidia deliciosa Root and Its Comparison with Conventional Extraction Methods
title_full_unstemmed Optimization of Microwave-Assisted Extraction of Antihepatotoxic Triterpenoid from Actinidia deliciosa Root and Its Comparison with Conventional Extraction Methods
title_short Optimization of Microwave-Assisted Extraction of Antihepatotoxic Triterpenoid from Actinidia deliciosa Root and Its Comparison with Conventional Extraction Methods
title_sort optimization of microwave assisted extraction of antihepatotoxic triterpenoid from actinidia deliciosa root and its comparison with conventional extraction methods
topic microwave-assisted extraction (MAE)
Actinidia deliciosa root
triterpenoid
response surface methodology
url http://hrcak.srce.hr/file/43769
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AT xinpengbai optimizationofmicrowaveassistedextractionofantihepatotoxictriterpenoidfromactinidiadeliciosarootanditscomparisonwithconventionalextractionmethods
AT aiyongqiu optimizationofmicrowaveassistedextractionofantihepatotoxictriterpenoidfromactinidiadeliciosarootanditscomparisonwithconventionalextractionmethods