Full-Length Transcriptome Analysis of the Ichthyotoxic Harmful Alga <i>Heterosigma akashiwo</i> (Raphidophyceae) Using Single-Molecule Real-Time Sequencing

The raphidophyte <i>Heterosigma akashiwo</i> is a harmful algal species. The bloom of this organism has been associated with the massive mortality of fish in many coastal waters. To investigate the molecular mechanism of <i>H</i>. <i>akashiwo</i> blooms, having a...

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Bibliographic Details
Main Authors: Nanjing Ji, Xueyao Yin, Yujiao Chen, Yifan Chen, Mingyang Xu, Jinwang Huang, Yuefeng Cai, Xin Shen
Format: Article
Language:English
Published: MDPI AG 2023-02-01
Series:Microorganisms
Subjects:
Online Access:https://www.mdpi.com/2076-2607/11/2/389
Description
Summary:The raphidophyte <i>Heterosigma akashiwo</i> is a harmful algal species. The bloom of this organism has been associated with the massive mortality of fish in many coastal waters. To investigate the molecular mechanism of <i>H</i>. <i>akashiwo</i> blooms, having a reliable reference transcriptome of this species is essential. Therefore, in this study, a full-length transcriptome of <i>H</i>. <i>akashiwo</i> was obtained by single-molecule real-time sequencing. In total, 45.44 Gb subread bases were generated, and 16,668 unigenes were obtained after the sequencing data processing. A total of 8666 (52.00%) unigenes were successfully annotated using seven public databases. Among them, mostly phosphorus and nitrogen metabolism genes were detected. Moreover, there were 300 putative transcription factors, 4392 putative long non-coding RNAs, and 7851 simple sequence repeats predicted. This study provides a valuable reference transcriptome for understanding how <i>H</i>. <i>akashiwo</i> blooms at a molecular level.
ISSN:2076-2607