Impact of a Multistrain Probiotic Formulation with High Bifidobacterial Content on the Fecal Bacterial Community and Short-Chain Fatty Acid Levels of Healthy Adults
The consumption of probiotic products is continually increasing, supported by growing scientific evidence of their efficacy. Considering that probiotics may primarily affect health (either positively or negatively) through gut microbiota modulation, the first aspect that should be evaluated is their...
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| Format: | Article |
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MDPI AG
2020-03-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/4/492 |
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| author | Giorgio Gargari Valentina Taverniti Ranjan Koirala Claudio Gardana Simone Guglielmetti |
| author_facet | Giorgio Gargari Valentina Taverniti Ranjan Koirala Claudio Gardana Simone Guglielmetti |
| author_sort | Giorgio Gargari |
| collection | DOAJ |
| description | The consumption of probiotic products is continually increasing, supported by growing scientific evidence of their efficacy. Considering that probiotics may primarily affect health (either positively or negatively) through gut microbiota modulation, the first aspect that should be evaluated is their impact on the intestinal microbial ecosystem. In this study, we longitudinally analyzed the bacterial taxonomic composition and organic acid levels in four fecal samples collected over the course of four weeks from 19 healthy adults who ingested one capsule a day for two weeks of a formulation containing at least 70 billion colony-forming units, consisting of 25% lactobacilli and 75% <i>Bifidobacterium animalis</i> subsp. <i>lactis</i>. We found that 16S rRNA gene profiling showed that probiotic intake only induced an increase in a single operational taxonomic unit ascribed to <i>B. animalis</i>, plausibly corresponding to the ingested bifidobacterial strain. Furthermore, liquid chromatography/mass spectrometry revealed a significant increase in the lactate and acetate/butyrate ratio and a trend toward a decrease in succinate following probiotic administration. The presented results indicate that the investigated probiotic formulation did not alter the intestinal bacterial ecosystem of healthy adults and suggest its potential ability to promote colonization resistance in the gut through a transient increase in fecal bifidobacteria, lactic acid, and the acetate/butyrate ratio. |
| first_indexed | 2024-03-10T20:47:00Z |
| format | Article |
| id | doaj.art-8bbc7cc6dce74b339943a90c9bcf4af2 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T20:47:00Z |
| publishDate | 2020-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-8bbc7cc6dce74b339943a90c9bcf4af22023-11-19T20:12:37ZengMDPI AGMicroorganisms2076-26072020-03-018449210.3390/microorganisms8040492Impact of a Multistrain Probiotic Formulation with High Bifidobacterial Content on the Fecal Bacterial Community and Short-Chain Fatty Acid Levels of Healthy AdultsGiorgio Gargari0Valentina Taverniti1Ranjan Koirala2Claudio Gardana3Simone Guglielmetti4Division of Food Microbiology and Bioprocesses, Department of Food, Environmental and Nutritional Sciences, University of Milan, 20133 Milan, ItalyDivision of Food Microbiology and Bioprocesses, Department of Food, Environmental and Nutritional Sciences, University of Milan, 20133 Milan, ItalyDivision of Food Microbiology and Bioprocesses, Department of Food, Environmental and Nutritional Sciences, University of Milan, 20133 Milan, ItalyDivision of Human Nutrition, Department of Food, Environmental and Nutritional Sciences, University of Milan, 20133 Milan, ItalyDivision of Food Microbiology and Bioprocesses, Department of Food, Environmental and Nutritional Sciences, University of Milan, 20133 Milan, ItalyThe consumption of probiotic products is continually increasing, supported by growing scientific evidence of their efficacy. Considering that probiotics may primarily affect health (either positively or negatively) through gut microbiota modulation, the first aspect that should be evaluated is their impact on the intestinal microbial ecosystem. In this study, we longitudinally analyzed the bacterial taxonomic composition and organic acid levels in four fecal samples collected over the course of four weeks from 19 healthy adults who ingested one capsule a day for two weeks of a formulation containing at least 70 billion colony-forming units, consisting of 25% lactobacilli and 75% <i>Bifidobacterium animalis</i> subsp. <i>lactis</i>. We found that 16S rRNA gene profiling showed that probiotic intake only induced an increase in a single operational taxonomic unit ascribed to <i>B. animalis</i>, plausibly corresponding to the ingested bifidobacterial strain. Furthermore, liquid chromatography/mass spectrometry revealed a significant increase in the lactate and acetate/butyrate ratio and a trend toward a decrease in succinate following probiotic administration. The presented results indicate that the investigated probiotic formulation did not alter the intestinal bacterial ecosystem of healthy adults and suggest its potential ability to promote colonization resistance in the gut through a transient increase in fecal bifidobacteria, lactic acid, and the acetate/butyrate ratio.https://www.mdpi.com/2076-2607/8/4/492DESeq2ALDEx2short-chain fatty acidssuccinateacetatebutyrate |
| spellingShingle | Giorgio Gargari Valentina Taverniti Ranjan Koirala Claudio Gardana Simone Guglielmetti Impact of a Multistrain Probiotic Formulation with High Bifidobacterial Content on the Fecal Bacterial Community and Short-Chain Fatty Acid Levels of Healthy Adults Microorganisms DESeq2 ALDEx2 short-chain fatty acids succinate acetate butyrate |
| title | Impact of a Multistrain Probiotic Formulation with High Bifidobacterial Content on the Fecal Bacterial Community and Short-Chain Fatty Acid Levels of Healthy Adults |
| title_full | Impact of a Multistrain Probiotic Formulation with High Bifidobacterial Content on the Fecal Bacterial Community and Short-Chain Fatty Acid Levels of Healthy Adults |
| title_fullStr | Impact of a Multistrain Probiotic Formulation with High Bifidobacterial Content on the Fecal Bacterial Community and Short-Chain Fatty Acid Levels of Healthy Adults |
| title_full_unstemmed | Impact of a Multistrain Probiotic Formulation with High Bifidobacterial Content on the Fecal Bacterial Community and Short-Chain Fatty Acid Levels of Healthy Adults |
| title_short | Impact of a Multistrain Probiotic Formulation with High Bifidobacterial Content on the Fecal Bacterial Community and Short-Chain Fatty Acid Levels of Healthy Adults |
| title_sort | impact of a multistrain probiotic formulation with high bifidobacterial content on the fecal bacterial community and short chain fatty acid levels of healthy adults |
| topic | DESeq2 ALDEx2 short-chain fatty acids succinate acetate butyrate |
| url | https://www.mdpi.com/2076-2607/8/4/492 |
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