Intravital electrochemical nanosensor as a tool for the measurement of reactive oxygen/nitrogen species in liver diseases
Abstract Reactive oxygen/nitrogen species (ROS/RNS) are formed during normal cellular metabolism and contribute to its regulation, while many pathological processes are associated with ROS/RNS imbalances. Modern methods for measuring ROS/RNS are mainly based on the use of inducible fluorescent dyes...
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| Format: | Article |
| Language: | English |
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BMC
2022-11-01
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| Series: | Journal of Nanobiotechnology |
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| Online Access: | https://doi.org/10.1186/s12951-022-01688-z |
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| author | Tatiana Abakumova Alexander Vaneev Victor Naumenko Arina Shokhina Vsevolod Belousov Arsen Mikaelyan Kamilla Balysheva Peter Gorelkin Alexander Erofeev Timofei Zatsepin |
| author_facet | Tatiana Abakumova Alexander Vaneev Victor Naumenko Arina Shokhina Vsevolod Belousov Arsen Mikaelyan Kamilla Balysheva Peter Gorelkin Alexander Erofeev Timofei Zatsepin |
| author_sort | Tatiana Abakumova |
| collection | DOAJ |
| description | Abstract Reactive oxygen/nitrogen species (ROS/RNS) are formed during normal cellular metabolism and contribute to its regulation, while many pathological processes are associated with ROS/RNS imbalances. Modern methods for measuring ROS/RNS are mainly based on the use of inducible fluorescent dyes and protein-based sensors, which have several disadvantages for in vivo use. Intravital electrochemical nanosensors can be used to quantify ROS/RNS with high sensitivity without exogenous tracers and allow dynamic ROS/RNS measurements in vivo. Here, we developed a method for quantifying total ROS/RNS levels in the liver and evaluated our setup in live mice using three common models of liver disease associated with ROS activation: acute liver injury with CCl4, partial hepatectomy (HE), and induced hepatocellular carcinoma (HCC). We have demonstrated using intravital electrochemical detection that any exposure to the peritoneum in vivo leads to an increase in total ROS/RNS levels, from a slight increase to an explosion, depending on the procedure. Analysis of the total ROS/RNS level in a partial hepatectomy model revealed oxidative stress, both in mice 24 h after HE and in sham-operated mice. We quantified dose-dependent ROS/RNS production in CCl4-induced injury with underlying neutrophil infiltration and cell death. We expect that in vivo electrochemical measurements of reactive oxygen/nitrogen species in the liver may become a routine approach that provides valuable data in research and preclinical studies. |
| first_indexed | 2024-04-11T13:53:51Z |
| format | Article |
| id | doaj.art-8bbccfdefa5e4bb091bb2d1bfd4d8dd5 |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-04-11T13:53:51Z |
| publishDate | 2022-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-8bbccfdefa5e4bb091bb2d1bfd4d8dd52022-12-22T04:20:25ZengBMCJournal of Nanobiotechnology1477-31552022-11-0120111510.1186/s12951-022-01688-zIntravital electrochemical nanosensor as a tool for the measurement of reactive oxygen/nitrogen species in liver diseasesTatiana Abakumova0Alexander Vaneev1Victor Naumenko2Arina Shokhina3Vsevolod Belousov4Arsen Mikaelyan5Kamilla Balysheva6Peter Gorelkin7Alexander Erofeev8Timofei Zatsepin9Skolkovo Institute of Science and TechnologyNational University of Science and Technology «MISIS»V. Serbsky National Medical Research Center for Psychiatry and NarcologyFederal Center of Brain Research and Neurotechnologies of the Federal Medical Biological AgencyFederal Center of Brain Research and Neurotechnologies of the Federal Medical Biological AgencyKoltzov Institute of Developmental Biology of Russian Academy of SciencesNational University of Science and Technology «MISIS»National University of Science and Technology «MISIS»National University of Science and Technology «MISIS»Lomonosov Moscow State UniversityAbstract Reactive oxygen/nitrogen species (ROS/RNS) are formed during normal cellular metabolism and contribute to its regulation, while many pathological processes are associated with ROS/RNS imbalances. Modern methods for measuring ROS/RNS are mainly based on the use of inducible fluorescent dyes and protein-based sensors, which have several disadvantages for in vivo use. Intravital electrochemical nanosensors can be used to quantify ROS/RNS with high sensitivity without exogenous tracers and allow dynamic ROS/RNS measurements in vivo. Here, we developed a method for quantifying total ROS/RNS levels in the liver and evaluated our setup in live mice using three common models of liver disease associated with ROS activation: acute liver injury with CCl4, partial hepatectomy (HE), and induced hepatocellular carcinoma (HCC). We have demonstrated using intravital electrochemical detection that any exposure to the peritoneum in vivo leads to an increase in total ROS/RNS levels, from a slight increase to an explosion, depending on the procedure. Analysis of the total ROS/RNS level in a partial hepatectomy model revealed oxidative stress, both in mice 24 h after HE and in sham-operated mice. We quantified dose-dependent ROS/RNS production in CCl4-induced injury with underlying neutrophil infiltration and cell death. We expect that in vivo electrochemical measurements of reactive oxygen/nitrogen species in the liver may become a routine approach that provides valuable data in research and preclinical studies.https://doi.org/10.1186/s12951-022-01688-zOxidative stressIntravital imagingElectrochemical detectionNanosensorsLiver diseaseHepatocellular carcinoma |
| spellingShingle | Tatiana Abakumova Alexander Vaneev Victor Naumenko Arina Shokhina Vsevolod Belousov Arsen Mikaelyan Kamilla Balysheva Peter Gorelkin Alexander Erofeev Timofei Zatsepin Intravital electrochemical nanosensor as a tool for the measurement of reactive oxygen/nitrogen species in liver diseases Journal of Nanobiotechnology Oxidative stress Intravital imaging Electrochemical detection Nanosensors Liver disease Hepatocellular carcinoma |
| title | Intravital electrochemical nanosensor as a tool for the measurement of reactive oxygen/nitrogen species in liver diseases |
| title_full | Intravital electrochemical nanosensor as a tool for the measurement of reactive oxygen/nitrogen species in liver diseases |
| title_fullStr | Intravital electrochemical nanosensor as a tool for the measurement of reactive oxygen/nitrogen species in liver diseases |
| title_full_unstemmed | Intravital electrochemical nanosensor as a tool for the measurement of reactive oxygen/nitrogen species in liver diseases |
| title_short | Intravital electrochemical nanosensor as a tool for the measurement of reactive oxygen/nitrogen species in liver diseases |
| title_sort | intravital electrochemical nanosensor as a tool for the measurement of reactive oxygen nitrogen species in liver diseases |
| topic | Oxidative stress Intravital imaging Electrochemical detection Nanosensors Liver disease Hepatocellular carcinoma |
| url | https://doi.org/10.1186/s12951-022-01688-z |
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