Genome-wide screening of mRNA expression in leprosy patients.
Leprosy, an infectious disease caused by Mycobacterium leprae, affects millions of people worldwide. However, little is known regarding its molecular pathophysiological mechanisms. In this study, a comprehensive assessment of human mRNA was performed on leprosy skin lesions by using DNA chip microar...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-11-01
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| Series: | Frontiers in Genetics |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fgene.2015.00334/full |
| _version_ | 1818904707184197632 |
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| author | Andrea Faria Fernandes Belone Andrea Faria Fernandes Belone Patrícia Sammarco Rosa Ana Paula Favaro Trombone Luciana Raquel Vincenzi Fachin Cássio César Guidella Somei eUra Jaison Antonio Barreto Mabel Gigliola Pinilla Alex Fiorini de Carvalho Dirce Maria Carraro Fernando Augusto Soares Cleverson Teixeira Soares |
| author_facet | Andrea Faria Fernandes Belone Andrea Faria Fernandes Belone Patrícia Sammarco Rosa Ana Paula Favaro Trombone Luciana Raquel Vincenzi Fachin Cássio César Guidella Somei eUra Jaison Antonio Barreto Mabel Gigliola Pinilla Alex Fiorini de Carvalho Dirce Maria Carraro Fernando Augusto Soares Cleverson Teixeira Soares |
| author_sort | Andrea Faria Fernandes Belone |
| collection | DOAJ |
| description | Leprosy, an infectious disease caused by Mycobacterium leprae, affects millions of people worldwide. However, little is known regarding its molecular pathophysiological mechanisms. In this study, a comprehensive assessment of human mRNA was performed on leprosy skin lesions by using DNA chip microarrays, which included the entire spectrum of the disease along with its reactional states. Sixty-six samples from leprotic lesions (10TT, 10BT, 10BB, 10BL, 4LL, 14R1, and 10R2) and nine skin biopsies from healthy individuals were used as controls (CC) (ages ranged from 06 to 83 years, 48 were male and 29 female). The evaluation identified 1,580 differentially expressed mRNAs [Fold Change (FC)≥2.0, p<0.05] in diseased lesions versus healthy controls. Some of these genes were observed in all forms of the disease (CD2, CD27, chit1, FA2H, FAM26F, GZMB, MMP9, SLAMF7, UBD) and others were exclusive to reactional forms (Type 1 reaction: GPNMB, IL1B, MICAL2, FOXQ1; Type 2 reaction: AKR1B10, FAM180B, FOXQ1, NNMT, NR1D1, PTX3, TNFRSF25). In literature, these mRNAs have been associated with numerous pathophysiological processes and signaling pathways and are present in a large number of diseases. The role of these mRNAs maybe studied in the context of developing new diagnostic markers and therapeutic targets for leprosy. |
| first_indexed | 2024-12-19T21:11:43Z |
| format | Article |
| id | doaj.art-8bbe2d76b1bc4b1bad3b86a825e9f9fa |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-19T21:11:43Z |
| publishDate | 2015-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-8bbe2d76b1bc4b1bad3b86a825e9f9fa2022-12-21T20:05:29ZengFrontiers Media S.A.Frontiers in Genetics1664-80212015-11-01610.3389/fgene.2015.00334164575Genome-wide screening of mRNA expression in leprosy patients.Andrea Faria Fernandes Belone0Andrea Faria Fernandes Belone1Patrícia Sammarco Rosa2Ana Paula Favaro Trombone3Luciana Raquel Vincenzi Fachin4Cássio César Guidella5Somei eUra6Jaison Antonio Barreto7Mabel Gigliola Pinilla8Alex Fiorini de Carvalho9Dirce Maria Carraro10Fernando Augusto Soares11Cleverson Teixeira Soares12Instituto Lauro de Souza LimaA.C. Camargo Cancer CenterInstituto Lauro de Souza LimaUniversidade do Sagrado CoraçãoInstituto Lauro de Souza LimaJardim Guanabara Health CenterInstituto Lauro de Souza LimaInstituto Lauro de Souza LimaHospital A.C.CamargoHospital A.C.CamargoHospital A.C.CamargoA.C. Camargo Cancer CenterInstituto Lauro de Souza LimaLeprosy, an infectious disease caused by Mycobacterium leprae, affects millions of people worldwide. However, little is known regarding its molecular pathophysiological mechanisms. In this study, a comprehensive assessment of human mRNA was performed on leprosy skin lesions by using DNA chip microarrays, which included the entire spectrum of the disease along with its reactional states. Sixty-six samples from leprotic lesions (10TT, 10BT, 10BB, 10BL, 4LL, 14R1, and 10R2) and nine skin biopsies from healthy individuals were used as controls (CC) (ages ranged from 06 to 83 years, 48 were male and 29 female). The evaluation identified 1,580 differentially expressed mRNAs [Fold Change (FC)≥2.0, p<0.05] in diseased lesions versus healthy controls. Some of these genes were observed in all forms of the disease (CD2, CD27, chit1, FA2H, FAM26F, GZMB, MMP9, SLAMF7, UBD) and others were exclusive to reactional forms (Type 1 reaction: GPNMB, IL1B, MICAL2, FOXQ1; Type 2 reaction: AKR1B10, FAM180B, FOXQ1, NNMT, NR1D1, PTX3, TNFRSF25). In literature, these mRNAs have been associated with numerous pathophysiological processes and signaling pathways and are present in a large number of diseases. The role of these mRNAs maybe studied in the context of developing new diagnostic markers and therapeutic targets for leprosy.http://journal.frontiersin.org/Journal/10.3389/fgene.2015.00334/fullLeprosyMicroarraymRNASignaling Pathwaysimmunohistochemistry (IHC) |
| spellingShingle | Andrea Faria Fernandes Belone Andrea Faria Fernandes Belone Patrícia Sammarco Rosa Ana Paula Favaro Trombone Luciana Raquel Vincenzi Fachin Cássio César Guidella Somei eUra Jaison Antonio Barreto Mabel Gigliola Pinilla Alex Fiorini de Carvalho Dirce Maria Carraro Fernando Augusto Soares Cleverson Teixeira Soares Genome-wide screening of mRNA expression in leprosy patients. Frontiers in Genetics Leprosy Microarray mRNA Signaling Pathways immunohistochemistry (IHC) |
| title | Genome-wide screening of mRNA expression in leprosy patients. |
| title_full | Genome-wide screening of mRNA expression in leprosy patients. |
| title_fullStr | Genome-wide screening of mRNA expression in leprosy patients. |
| title_full_unstemmed | Genome-wide screening of mRNA expression in leprosy patients. |
| title_short | Genome-wide screening of mRNA expression in leprosy patients. |
| title_sort | genome wide screening of mrna expression in leprosy patients |
| topic | Leprosy Microarray mRNA Signaling Pathways immunohistochemistry (IHC) |
| url | http://journal.frontiersin.org/Journal/10.3389/fgene.2015.00334/full |
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