CD200 and CD200R Expression on Peripheral Blood Lymphocytes and Serum CD200 Concentration as a New Marker of Endometriosis
The causes of endometriosis (EMS) remain unknown; however, a number of immunological abnormalities contribute to the pathogenesis of the disease. The cluster of differentiation-200 (CD200) and its receptor (CD200R) maintain peripheral self-tolerance by negatively regulating immune responses. In this...
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MDPI AG
2020-09-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/9/9/3035 |
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| author | Monika Abramiuk Ewelina Grywalska Izabela Korona-Głowniak Paulina Niedźwiedzka-Rystwej Grzegorz Polak Jan Kotarski Jacek Roliński |
| author_facet | Monika Abramiuk Ewelina Grywalska Izabela Korona-Głowniak Paulina Niedźwiedzka-Rystwej Grzegorz Polak Jan Kotarski Jacek Roliński |
| author_sort | Monika Abramiuk |
| collection | DOAJ |
| description | The causes of endometriosis (EMS) remain unknown; however, a number of immunological abnormalities contribute to the pathogenesis of the disease. The cluster of differentiation-200 (CD200) and its receptor (CD200R) maintain peripheral self-tolerance by negatively regulating immune responses. In this comparative cross-sectional study, we investigated the expression of CD200 and CD200R on T and B lymphocytes and the serum level of soluble CD200 (sCD200) using flow cytometry and ELISA, respectively. Peripheral blood samples were collected from 54 female patients and 20 healthy, age-matched controls. Results were tested for correlation with disease severity and selected clinical parameters. We demonstrated that the differences in sCD200 levels (<i>p</i> = 0.001), the frequencies of CD200-positive T and B lymphocytes (<i>p</i> < 0.001 and <i>p</i> = 0.004, respectively), and the frequencies of CD200R-positive T and B lymphocytes (<i>p</i> < 0.001 for all comparisons) in the study group correlated positively with disease severity. Receiver operating characteristic (ROC) analysis indicated that aberrant expression of CD200/CD200R might serve as a marker to distinguish between EMS cases. Finally, negative co-stimulatory factors may contribute to the induction and persistence of inflammation associated with EMS. It seems that it is essential to determine whether alteration in the CD200/CD200R pathway can be therapeutically targeted in EMS. |
| first_indexed | 2024-03-10T16:10:48Z |
| format | Article |
| id | doaj.art-8bc36c146b2848b492e1312ebecb8532 |
| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-10T16:10:48Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-8bc36c146b2848b492e1312ebecb85322023-11-20T14:29:13ZengMDPI AGJournal of Clinical Medicine2077-03832020-09-0199303510.3390/jcm9093035CD200 and CD200R Expression on Peripheral Blood Lymphocytes and Serum CD200 Concentration as a New Marker of EndometriosisMonika Abramiuk0Ewelina Grywalska1Izabela Korona-Głowniak2Paulina Niedźwiedzka-Rystwej3Grzegorz Polak4Jan Kotarski5Jacek Roliński61st Department of Gynecological Oncology and Gynecology, Medical University of Lublin, 20-081 Lublin, PolandDepartment of Clinical Immunology and Immunotherapy, Medical University of Lublin, 20-093 Lublin, PolandDepartment of Pharmaceutical Microbiology, Medical University of Lublin, Chodzki 1, 20-093 Lublin, PolandInstitute of Biology, University of Szczecin, 71-412 Szczecin, Poland1st Department of Gynecological Oncology and Gynecology, Medical University of Lublin, 20-081 Lublin, Poland1st Department of Gynecological Oncology and Gynecology, Medical University of Lublin, 20-081 Lublin, PolandDepartment of Clinical Immunology and Immunotherapy, Medical University of Lublin, 20-093 Lublin, PolandThe causes of endometriosis (EMS) remain unknown; however, a number of immunological abnormalities contribute to the pathogenesis of the disease. The cluster of differentiation-200 (CD200) and its receptor (CD200R) maintain peripheral self-tolerance by negatively regulating immune responses. In this comparative cross-sectional study, we investigated the expression of CD200 and CD200R on T and B lymphocytes and the serum level of soluble CD200 (sCD200) using flow cytometry and ELISA, respectively. Peripheral blood samples were collected from 54 female patients and 20 healthy, age-matched controls. Results were tested for correlation with disease severity and selected clinical parameters. We demonstrated that the differences in sCD200 levels (<i>p</i> = 0.001), the frequencies of CD200-positive T and B lymphocytes (<i>p</i> < 0.001 and <i>p</i> = 0.004, respectively), and the frequencies of CD200R-positive T and B lymphocytes (<i>p</i> < 0.001 for all comparisons) in the study group correlated positively with disease severity. Receiver operating characteristic (ROC) analysis indicated that aberrant expression of CD200/CD200R might serve as a marker to distinguish between EMS cases. Finally, negative co-stimulatory factors may contribute to the induction and persistence of inflammation associated with EMS. It seems that it is essential to determine whether alteration in the CD200/CD200R pathway can be therapeutically targeted in EMS.https://www.mdpi.com/2077-0383/9/9/3035endometriosisCD200CD200Rnegative co-stimulationnon-invasive markers |
| spellingShingle | Monika Abramiuk Ewelina Grywalska Izabela Korona-Głowniak Paulina Niedźwiedzka-Rystwej Grzegorz Polak Jan Kotarski Jacek Roliński CD200 and CD200R Expression on Peripheral Blood Lymphocytes and Serum CD200 Concentration as a New Marker of Endometriosis Journal of Clinical Medicine endometriosis CD200 CD200R negative co-stimulation non-invasive markers |
| title | CD200 and CD200R Expression on Peripheral Blood Lymphocytes and Serum CD200 Concentration as a New Marker of Endometriosis |
| title_full | CD200 and CD200R Expression on Peripheral Blood Lymphocytes and Serum CD200 Concentration as a New Marker of Endometriosis |
| title_fullStr | CD200 and CD200R Expression on Peripheral Blood Lymphocytes and Serum CD200 Concentration as a New Marker of Endometriosis |
| title_full_unstemmed | CD200 and CD200R Expression on Peripheral Blood Lymphocytes and Serum CD200 Concentration as a New Marker of Endometriosis |
| title_short | CD200 and CD200R Expression on Peripheral Blood Lymphocytes and Serum CD200 Concentration as a New Marker of Endometriosis |
| title_sort | cd200 and cd200r expression on peripheral blood lymphocytes and serum cd200 concentration as a new marker of endometriosis |
| topic | endometriosis CD200 CD200R negative co-stimulation non-invasive markers |
| url | https://www.mdpi.com/2077-0383/9/9/3035 |
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