Microbial deproteinization of shrimp shell penaeus merguiensis for chitin extraction
Introduction: After cellulose, Chitin is the most abundant biopolymer in nature. The most important derivative of chitin is chitosan, obtained by deacetylation of chitin. Major sources of chitin are the exoskeleton of marine crustaceans such as crab, shrimp, and krill. Chitin extraction from shrimp...
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| Format: | Article |
| Language: | English |
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University of Isfahan
2016-09-01
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| Series: | Biological Journal of Microorganism |
| Subjects: | |
| Online Access: | http://uijs.ui.ac.ir/bjm/browse.php?a_code=A-10-689-1&slc_lang=en&sid=1 |
| _version_ | 1819149418686840832 |
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| author | Fatemeh Sedaghat Morteza Yousefzadi Hojjat Toiserkani Sohrab Najafipour |
| author_facet | Fatemeh Sedaghat Morteza Yousefzadi Hojjat Toiserkani Sohrab Najafipour |
| author_sort | Fatemeh Sedaghat |
| collection | DOAJ |
| description | Introduction: After cellulose, Chitin is the most abundant biopolymer in nature. The most important derivative of chitin is chitosan, obtained by deacetylation of chitin. Major sources of chitin are the exoskeleton of marine crustaceans such as crab, shrimp, and krill. Chitin extraction from shrimp shells can be carried out chemically or using biological methods. Microbial fermentation as an eco-friendly procedure is a suitable alternative for the chemical and enzymatic processes. In this study, the effect of three protease-producing bacteria species (Pseudomonas aeruginosa, Serratia marcescens, and Bacillus pumilus) on the efficiency of microbial demineralization (DM) and deproteinization (DP) of the shrimp shell penaeus merguiensis, was investigated. Furthermore, the antioxidant activity of hydrolysate obtained during the fermentation process was measured.
Materials and methods: Demineralization and deproteinization was carried out by incubating shrimp waste inoculated with bacteria at 30°C and 100 rpm for 6 days.
Results: Statistical analysis of data showed a significant difference between the percentage of demineralization and deproteinization in different bacteria species (p<0.05). The highest deproteinization (74.76%) and demineralization rate (78.46%) were obtained with P. aeruginosa, while the lowest was observed for S. marcescens. Antioxidant activity of hydrolysate also showed a significant difference. The highest reducing power and total antioxidant capacity were observed in volumes of 400 µl hydrolysate of S.marcescens and 100 µl hydrolysate of B. pumilus, respectively.
Discussion and conclusion: The results indicated that P. aeruginosa in comparison with other bacterial strains, had a higher ability to remove proteins and minerals from shrimp shell waste. Therefore, the use of this bacterium is suitable for protein and minerals removal from marine crustaceans. |
| first_indexed | 2024-12-22T14:01:18Z |
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| institution | Directory Open Access Journal |
| issn | 2322-5173 2322-5181 |
| language | English |
| last_indexed | 2024-12-22T14:01:18Z |
| publishDate | 2016-09-01 |
| publisher | University of Isfahan |
| record_format | Article |
| series | Biological Journal of Microorganism |
| spelling | doaj.art-8bc70d4099964d9c957643bb6bcddab52022-12-21T18:23:25ZengUniversity of IsfahanBiological Journal of Microorganism2322-51732322-51812016-09-01518141152Microbial deproteinization of shrimp shell penaeus merguiensis for chitin extractionFatemeh Sedaghat0Morteza Yousefzadi1Hojjat Toiserkani2Sohrab Najafipour3 کارشناسی ارشد زیستشناسی دریا، دانشگاه هرمزگان، ایران دانشیار زیستشناسی، دانشگاه هرمزگان، ایران دانشیار شیمی، دانشگاه هرمزگان، ایران استادیار میکروبیولوژی، دانشگاه علوم پزشکی فسا، ایران Introduction: After cellulose, Chitin is the most abundant biopolymer in nature. The most important derivative of chitin is chitosan, obtained by deacetylation of chitin. Major sources of chitin are the exoskeleton of marine crustaceans such as crab, shrimp, and krill. Chitin extraction from shrimp shells can be carried out chemically or using biological methods. Microbial fermentation as an eco-friendly procedure is a suitable alternative for the chemical and enzymatic processes. In this study, the effect of three protease-producing bacteria species (Pseudomonas aeruginosa, Serratia marcescens, and Bacillus pumilus) on the efficiency of microbial demineralization (DM) and deproteinization (DP) of the shrimp shell penaeus merguiensis, was investigated. Furthermore, the antioxidant activity of hydrolysate obtained during the fermentation process was measured. Materials and methods: Demineralization and deproteinization was carried out by incubating shrimp waste inoculated with bacteria at 30°C and 100 rpm for 6 days. Results: Statistical analysis of data showed a significant difference between the percentage of demineralization and deproteinization in different bacteria species (p<0.05). The highest deproteinization (74.76%) and demineralization rate (78.46%) were obtained with P. aeruginosa, while the lowest was observed for S. marcescens. Antioxidant activity of hydrolysate also showed a significant difference. The highest reducing power and total antioxidant capacity were observed in volumes of 400 µl hydrolysate of S.marcescens and 100 µl hydrolysate of B. pumilus, respectively. Discussion and conclusion: The results indicated that P. aeruginosa in comparison with other bacterial strains, had a higher ability to remove proteins and minerals from shrimp shell waste. Therefore, the use of this bacterium is suitable for protein and minerals removal from marine crustaceans.http://uijs.ui.ac.ir/bjm/browse.php?a_code=A-10-689-1&slc_lang=en&sid=1Chitin Shrimp shell Microbial fermentation Deproteinization Demineralization Pseudomonas aeruginosa |
| spellingShingle | Fatemeh Sedaghat Morteza Yousefzadi Hojjat Toiserkani Sohrab Najafipour Microbial deproteinization of shrimp shell penaeus merguiensis for chitin extraction Biological Journal of Microorganism Chitin Shrimp shell Microbial fermentation Deproteinization Demineralization Pseudomonas aeruginosa |
| title | Microbial deproteinization of shrimp shell penaeus merguiensis for chitin extraction |
| title_full | Microbial deproteinization of shrimp shell penaeus merguiensis for chitin extraction |
| title_fullStr | Microbial deproteinization of shrimp shell penaeus merguiensis for chitin extraction |
| title_full_unstemmed | Microbial deproteinization of shrimp shell penaeus merguiensis for chitin extraction |
| title_short | Microbial deproteinization of shrimp shell penaeus merguiensis for chitin extraction |
| title_sort | microbial deproteinization of shrimp shell penaeus merguiensis for chitin extraction |
| topic | Chitin Shrimp shell Microbial fermentation Deproteinization Demineralization Pseudomonas aeruginosa |
| url | http://uijs.ui.ac.ir/bjm/browse.php?a_code=A-10-689-1&slc_lang=en&sid=1 |
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