16S rRNA analysis of diversity of manure microbial community in dairy farm environment.
Dairy farms generate a considerable amount of manure, which is applied in cropland as fertilizer. While the use of manure as fertilizer reduces the application of chemical fertilizers, the main concern with regards to manure application is microbial pollution. Manure is a reservoir of a broad range...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2018-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0190126 |
| _version_ | 1831788570241662976 |
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| author | Pramod Pandey Colleen Chiu Max Miao Yi Wang Matthew Settles Noelia Silva Del Rio Alejandro Castillo Alex Souza Richard Pereira Richard Jeannotte |
| author_facet | Pramod Pandey Colleen Chiu Max Miao Yi Wang Matthew Settles Noelia Silva Del Rio Alejandro Castillo Alex Souza Richard Pereira Richard Jeannotte |
| author_sort | Pramod Pandey |
| collection | DOAJ |
| description | Dairy farms generate a considerable amount of manure, which is applied in cropland as fertilizer. While the use of manure as fertilizer reduces the application of chemical fertilizers, the main concern with regards to manure application is microbial pollution. Manure is a reservoir of a broad range of microbial populations, including pathogens, which have potential to cause contamination and pose risks to public and animal health. Despite the widespread use of manure fertilizer, the change in microbial diversity of manure under various treatment processes is still not well-understood. We hypothesize that the microbial population of animal waste changes with manure handling used in a farm environment. Consequential microbial risk caused by animal manure may depend on manure handling. In this study, a reconnaissance effort for sampling dairy manure in California Central Valley followed by 16S rRNA analysis of content and diversity was undertaken to understand the microbiome of manure after various handling processes. The microbial community analysis of manure revealed that the population in liquid manure differs from that in solid manure. For instance, the bacteria of genus Sulfuriomonas were unique in liquid samples, while the bacteria of genus Thermos were observed only in solid samples. Bacteria of genus Clostridium were present in both solid and liquid samples. The population among liquid samples was comparable, as was the population among solid samples. These findings suggest that the mode of manure application (i.e., liquid versus solid) could have a potential impact on the microbiome of cropland receiving manure as fertilizers. |
| first_indexed | 2024-12-22T13:52:08Z |
| format | Article |
| id | doaj.art-8bc7daea6b704ef4adca86f331d7a3f7 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T13:52:08Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-8bc7daea6b704ef4adca86f331d7a3f72022-12-21T18:23:39ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01131e019012610.1371/journal.pone.019012616S rRNA analysis of diversity of manure microbial community in dairy farm environment.Pramod PandeyColleen ChiuMax MiaoYi WangMatthew SettlesNoelia Silva Del RioAlejandro CastilloAlex SouzaRichard PereiraRichard JeannotteDairy farms generate a considerable amount of manure, which is applied in cropland as fertilizer. While the use of manure as fertilizer reduces the application of chemical fertilizers, the main concern with regards to manure application is microbial pollution. Manure is a reservoir of a broad range of microbial populations, including pathogens, which have potential to cause contamination and pose risks to public and animal health. Despite the widespread use of manure fertilizer, the change in microbial diversity of manure under various treatment processes is still not well-understood. We hypothesize that the microbial population of animal waste changes with manure handling used in a farm environment. Consequential microbial risk caused by animal manure may depend on manure handling. In this study, a reconnaissance effort for sampling dairy manure in California Central Valley followed by 16S rRNA analysis of content and diversity was undertaken to understand the microbiome of manure after various handling processes. The microbial community analysis of manure revealed that the population in liquid manure differs from that in solid manure. For instance, the bacteria of genus Sulfuriomonas were unique in liquid samples, while the bacteria of genus Thermos were observed only in solid samples. Bacteria of genus Clostridium were present in both solid and liquid samples. The population among liquid samples was comparable, as was the population among solid samples. These findings suggest that the mode of manure application (i.e., liquid versus solid) could have a potential impact on the microbiome of cropland receiving manure as fertilizers.https://doi.org/10.1371/journal.pone.0190126 |
| spellingShingle | Pramod Pandey Colleen Chiu Max Miao Yi Wang Matthew Settles Noelia Silva Del Rio Alejandro Castillo Alex Souza Richard Pereira Richard Jeannotte 16S rRNA analysis of diversity of manure microbial community in dairy farm environment. PLoS ONE |
| title | 16S rRNA analysis of diversity of manure microbial community in dairy farm environment. |
| title_full | 16S rRNA analysis of diversity of manure microbial community in dairy farm environment. |
| title_fullStr | 16S rRNA analysis of diversity of manure microbial community in dairy farm environment. |
| title_full_unstemmed | 16S rRNA analysis of diversity of manure microbial community in dairy farm environment. |
| title_short | 16S rRNA analysis of diversity of manure microbial community in dairy farm environment. |
| title_sort | 16s rrna analysis of diversity of manure microbial community in dairy farm environment |
| url | https://doi.org/10.1371/journal.pone.0190126 |
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