Sperm motility and velocities of Characiformes fishes in different times post-activation
<p class="Pa7">The objective of the current study was to observe the performance kinetics (motilities and velocities) of the spermatozoa from <em>Prochilodus brevis </em>(curimatã), <em>Colossoma macropomum </em>(tambaqui) and <em>Piaractus brachypomus &...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Universidade Estadual de Londrina
2015-12-01
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| Series: | Semina: Ciências Agrárias |
| Subjects: | |
| Online Access: | http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/19631 |
| _version_ | 1817992801220034560 |
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| author | Mônica Aline Parente Melo-Maciel José Ferreira Nunes João Paulo Silva Pinheiro Larissa Teixeira Nunes Romulo Roberto Ribeiro Pinheiro Júlia Trugilio Lopes Carminda Sandra Brito Salmito-Vanderley |
| author_facet | Mônica Aline Parente Melo-Maciel José Ferreira Nunes João Paulo Silva Pinheiro Larissa Teixeira Nunes Romulo Roberto Ribeiro Pinheiro Júlia Trugilio Lopes Carminda Sandra Brito Salmito-Vanderley |
| author_sort | Mônica Aline Parente Melo-Maciel |
| collection | DOAJ |
| description | <p class="Pa7">The objective of the current study was to observe the performance kinetics (motilities and velocities) of the spermatozoa from <em>Prochilodus brevis </em>(curimatã), <em>Colossoma macropomum </em>(tambaqui) and <em>Piaractus brachypomus </em>(pirapitinga) species in different times post-activation. The sperm of <em>P. brevis</em>, <em>C. macropomum </em>and <em>P. brachypomus </em>species were collected after hormonal induction with carp pituitary extract. The samples with not contamination with water, urine or feces had motility subjective, morphology, osmolality and concentration analyzed. The samples selected were analyzed with Sperm Class Analyzer. Spermatozoa motility and velocities were captured at 10, 30, 60 and 120 s post-activation. No significant differences in total motility of <em>P. brevis </em>spermatozoa were observed between 10 s and 30 s post-activation. However, significant reduction was observed in 60 s. This reduction was more accentuated after 120 s. The same pattern of spermatozoa motility decline happened for <em>C. macropomum </em>and <em>P. brachypomus</em>. Velocities also followed the same pattern for the three species. There was significant reduction in velocities after 30 s; this reduction was more significant after 60 s. There was no significance difference between 60 s and 120 s post-activation. Sperm of <em>C. macropomum </em>and <em>P. brachypomus </em>show satisfactory sperm quality up to 60 s after activation. On the other hand, sperm of <em>P. brevis </em>up to 120 s after activation. These findings show that the rate of sperm motility in different times post activation is change for each species tested.</p> |
| first_indexed | 2024-04-14T01:31:07Z |
| format | Article |
| id | doaj.art-8bce18b9dc314460b49cfb591dd1630c |
| institution | Directory Open Access Journal |
| issn | 1676-546X 1679-0359 |
| language | English |
| last_indexed | 2024-04-14T01:31:07Z |
| publishDate | 2015-12-01 |
| publisher | Universidade Estadual de Londrina |
| record_format | Article |
| series | Semina: Ciências Agrárias |
| spelling | doaj.art-8bce18b9dc314460b49cfb591dd1630c2022-12-22T02:20:10ZengUniversidade Estadual de LondrinaSemina: Ciências Agrárias1676-546X1679-03592015-12-013664023403010.5433/1679-0359.2015v36n6p402312707Sperm motility and velocities of Characiformes fishes in different times post-activationMônica Aline Parente Melo-Maciel0José Ferreira Nunes1João Paulo Silva Pinheiro2Larissa Teixeira Nunes3Romulo Roberto Ribeiro Pinheiro4Júlia Trugilio Lopes5Carminda Sandra Brito Salmito-Vanderley6Universidade Estadual do CearáUniversidade Estadual do CearáUniversidade Estadual do CearáUniversidade Estadual do CearáUniversidade Estadual do CearáUniversidade Estadual do CearáUniversidade Estadual do Ceará<p class="Pa7">The objective of the current study was to observe the performance kinetics (motilities and velocities) of the spermatozoa from <em>Prochilodus brevis </em>(curimatã), <em>Colossoma macropomum </em>(tambaqui) and <em>Piaractus brachypomus </em>(pirapitinga) species in different times post-activation. The sperm of <em>P. brevis</em>, <em>C. macropomum </em>and <em>P. brachypomus </em>species were collected after hormonal induction with carp pituitary extract. The samples with not contamination with water, urine or feces had motility subjective, morphology, osmolality and concentration analyzed. The samples selected were analyzed with Sperm Class Analyzer. Spermatozoa motility and velocities were captured at 10, 30, 60 and 120 s post-activation. No significant differences in total motility of <em>P. brevis </em>spermatozoa were observed between 10 s and 30 s post-activation. However, significant reduction was observed in 60 s. This reduction was more accentuated after 120 s. The same pattern of spermatozoa motility decline happened for <em>C. macropomum </em>and <em>P. brachypomus</em>. Velocities also followed the same pattern for the three species. There was significant reduction in velocities after 30 s; this reduction was more significant after 60 s. There was no significance difference between 60 s and 120 s post-activation. Sperm of <em>C. macropomum </em>and <em>P. brachypomus </em>show satisfactory sperm quality up to 60 s after activation. On the other hand, sperm of <em>P. brevis </em>up to 120 s after activation. These findings show that the rate of sperm motility in different times post activation is change for each species tested.</p>http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/19631Colossoma macropomumPiaractus brachypomusProchilodus brevisSperm class analyzer. |
| spellingShingle | Mônica Aline Parente Melo-Maciel José Ferreira Nunes João Paulo Silva Pinheiro Larissa Teixeira Nunes Romulo Roberto Ribeiro Pinheiro Júlia Trugilio Lopes Carminda Sandra Brito Salmito-Vanderley Sperm motility and velocities of Characiformes fishes in different times post-activation Semina: Ciências Agrárias Colossoma macropomum Piaractus brachypomus Prochilodus brevis Sperm class analyzer. |
| title | Sperm motility and velocities of Characiformes fishes in different times post-activation |
| title_full | Sperm motility and velocities of Characiformes fishes in different times post-activation |
| title_fullStr | Sperm motility and velocities of Characiformes fishes in different times post-activation |
| title_full_unstemmed | Sperm motility and velocities of Characiformes fishes in different times post-activation |
| title_short | Sperm motility and velocities of Characiformes fishes in different times post-activation |
| title_sort | sperm motility and velocities of characiformes fishes in different times post activation |
| topic | Colossoma macropomum Piaractus brachypomus Prochilodus brevis Sperm class analyzer. |
| url | http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/19631 |
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