Dead but functional liquid nitrogen‐treated umbilical cord mesenchymal stem cells accelerate full‐thickness skin wound healing
Abstract Background The direct application of umbilical cord‐derived mesenchymal stem cells (UCMSCs) for promoting skin wound healing and regeneration is challenging due to strict maintenance requirements and unpredictable differentiation results. Methods We developed dead but functional liquid nitr...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-08-01
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| Series: | Clinical and Translational Discovery |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/ctd2.193 |
| _version_ | 1797736149304737792 |
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| author | Yan Yang Yuqing Feng Meirong Hu Jingxian Deng Siying Wei Zhaoyang Wang Lu Li Rufei Huang Yuxin Wang Yuan Liu Tao Ye Yadong Huang |
| author_facet | Yan Yang Yuqing Feng Meirong Hu Jingxian Deng Siying Wei Zhaoyang Wang Lu Li Rufei Huang Yuxin Wang Yuan Liu Tao Ye Yadong Huang |
| author_sort | Yan Yang |
| collection | DOAJ |
| description | Abstract Background The direct application of umbilical cord‐derived mesenchymal stem cells (UCMSCs) for promoting skin wound healing and regeneration is challenging due to strict maintenance requirements and unpredictable differentiation results. Methods We developed dead but functional liquid nitrogen‐treated UCMSCs (LNT‐MSCs) by rapidly immersing live UCMSCs (live‐MSCs) in liquid nitrogen. Results The LNT‐MSCs maintained similar cellular structures and surface markers to those of live‐MSCs. We evaluated the therapeutic effects of both live‐MSCs and LNT‐MSCs on full‐thickness skin wound healing in rats. Our results showed that the LNT‐MSCs accelerated wound closure by enhancing the proliferation and migration of skin cells, promoting angiogenesis, and inducing a favourable macrophage phenotype shift. The regenerative healing effect of LNT‐MSCs was comparable to that of live‐MSCs, making them a potential alternative strategy for accelerating wound closure that avoids unpredictable differentiation results and creates a ready‐to‐use cell bank for clinical applications. |
| first_indexed | 2024-03-12T13:09:25Z |
| format | Article |
| id | doaj.art-8bce363dc4b74b2f94d35f71af71aa66 |
| institution | Directory Open Access Journal |
| issn | 2768-0622 |
| language | English |
| last_indexed | 2024-03-12T13:09:25Z |
| publishDate | 2023-08-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Translational Discovery |
| spelling | doaj.art-8bce363dc4b74b2f94d35f71af71aa662023-08-28T06:44:32ZengWileyClinical and Translational Discovery2768-06222023-08-0134n/an/a10.1002/ctd2.193Dead but functional liquid nitrogen‐treated umbilical cord mesenchymal stem cells accelerate full‐thickness skin wound healingYan Yang0Yuqing Feng1Meirong Hu2Jingxian Deng3Siying Wei4Zhaoyang Wang5Lu Li6Rufei Huang7Yuxin Wang8Yuan Liu9Tao Ye10Yadong Huang11Department of Cell Biology Jinan University Guangzhou ChinaDepartment of Pharmacology Jinan University Guangzhou ChinaDepartment of Cell Biology Jinan University Guangzhou ChinaDepartment of Pharmacology Jinan University Guangzhou ChinaDepartment of Cell Biology Jinan University Guangzhou ChinaDepartment of Cell Biology Jinan University Guangzhou ChinaDepartment of Cell Biology Jinan University Guangzhou ChinaDepartment of Cell Biology Jinan University Guangzhou ChinaDepartment of Cell Biology Jinan University Guangzhou ChinaDepartment of Cell Biology Jinan University Guangzhou ChinaDepartment of Cell Biology Jinan University Guangzhou ChinaDepartment of Cell Biology Jinan University Guangzhou ChinaAbstract Background The direct application of umbilical cord‐derived mesenchymal stem cells (UCMSCs) for promoting skin wound healing and regeneration is challenging due to strict maintenance requirements and unpredictable differentiation results. Methods We developed dead but functional liquid nitrogen‐treated UCMSCs (LNT‐MSCs) by rapidly immersing live UCMSCs (live‐MSCs) in liquid nitrogen. Results The LNT‐MSCs maintained similar cellular structures and surface markers to those of live‐MSCs. We evaluated the therapeutic effects of both live‐MSCs and LNT‐MSCs on full‐thickness skin wound healing in rats. Our results showed that the LNT‐MSCs accelerated wound closure by enhancing the proliferation and migration of skin cells, promoting angiogenesis, and inducing a favourable macrophage phenotype shift. The regenerative healing effect of LNT‐MSCs was comparable to that of live‐MSCs, making them a potential alternative strategy for accelerating wound closure that avoids unpredictable differentiation results and creates a ready‐to‐use cell bank for clinical applications.https://doi.org/10.1002/ctd2.193cryo‐shockingumbilical cord‐derived mesenchymal stem cellwound healing |
| spellingShingle | Yan Yang Yuqing Feng Meirong Hu Jingxian Deng Siying Wei Zhaoyang Wang Lu Li Rufei Huang Yuxin Wang Yuan Liu Tao Ye Yadong Huang Dead but functional liquid nitrogen‐treated umbilical cord mesenchymal stem cells accelerate full‐thickness skin wound healing Clinical and Translational Discovery cryo‐shocking umbilical cord‐derived mesenchymal stem cell wound healing |
| title | Dead but functional liquid nitrogen‐treated umbilical cord mesenchymal stem cells accelerate full‐thickness skin wound healing |
| title_full | Dead but functional liquid nitrogen‐treated umbilical cord mesenchymal stem cells accelerate full‐thickness skin wound healing |
| title_fullStr | Dead but functional liquid nitrogen‐treated umbilical cord mesenchymal stem cells accelerate full‐thickness skin wound healing |
| title_full_unstemmed | Dead but functional liquid nitrogen‐treated umbilical cord mesenchymal stem cells accelerate full‐thickness skin wound healing |
| title_short | Dead but functional liquid nitrogen‐treated umbilical cord mesenchymal stem cells accelerate full‐thickness skin wound healing |
| title_sort | dead but functional liquid nitrogen treated umbilical cord mesenchymal stem cells accelerate full thickness skin wound healing |
| topic | cryo‐shocking umbilical cord‐derived mesenchymal stem cell wound healing |
| url | https://doi.org/10.1002/ctd2.193 |
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