Immunogenicity of heparin-binding hemagglutinin expressed by Pichia pastoris GS115 strain
Objective(s): Heparin-binding hemagglutinin (HBHA), a mycobacterial cell surface protein, mediates adhesion to nonphagocytic cells and the dissemination of Mycobacterium tuberculosis (M. tuberculosis) from the site of primary infection. Superior expression systems are required to obtain abundant M....
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2018-02-01
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| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | http://ijbms.mums.ac.ir/article_10107_84f37b7d3c2befe07e5c8149094f6015.pdf |
| _version_ | 1818229628777529344 |
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| author | Xindong Teng Xiaoguang Chen Ke Zhu Hefei Xu |
| author_facet | Xindong Teng Xiaoguang Chen Ke Zhu Hefei Xu |
| author_sort | Xindong Teng |
| collection | DOAJ |
| description | Objective(s): Heparin-binding hemagglutinin (HBHA), a mycobacterial cell surface protein, mediates adhesion to nonphagocytic cells and the dissemination of Mycobacterium tuberculosis (M. tuberculosis) from the site of primary infection. Superior expression systems are required to obtain abundant M. tuberculosis proteins for the purpose of diagnosing M. tuberculosis infection or for the immunization. Here, HBHA was expressed by Pichia pastoris (P. pastoris) GS115 strain , and the immunogenicity of HBHA was evaluated. Materials and Methods: The HBHA gene of M. tuberculosis was cloned into the pPIC9K plasmid, which was good for electroporation into P. pastoris GS115 strain. Unlabeled HBHA protein was purified using a Sepharose CL-6B column, and its expression was confirmed using anti-HBHA polyclonal antibody from mouse serum. We injected C57BL/6 mice with HBHA/ dimethyldioctadecylammonium/trehalose 6,6′-dibehenate (HBHA/DDA/TDB) to investigate the immunogenicity of this potential vaccine. Results: The results demonstrated that HBHA/DDA/TDB has the ability to induce high levels of HBHA-specific IgG antibody and its subclasses, as well as interferon-gamma, compared with injection of phosphate-buffered saline, DDA/TDB alone and Bacillus Calmette-Guérin (BCG) controls (P |
| first_indexed | 2024-12-12T10:21:38Z |
| format | Article |
| id | doaj.art-8bcf96ec2d0546d18623d941d44e4755 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-12T10:21:38Z |
| publishDate | 2018-02-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-8bcf96ec2d0546d18623d941d44e47552022-12-22T00:27:33ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742018-02-0121221922410.22038/ijbms.2018.24280.606410107Immunogenicity of heparin-binding hemagglutinin expressed by Pichia pastoris GS115 strainXindong Teng0Xiaoguang Chen1Ke Zhu2Hefei Xu3Department of Clinical Laboratory, Shandong International Travel Healthcare Center, Qingdao, ChinaDepartment of Clinical Laboratory, Shandong International Travel Healthcare Center, Qingdao, ChinaDepartment of Clinical Laboratory, Shandong International Travel Healthcare Center, Qingdao, ChinaDepartment of Clinical Laboratory, Shandong International Travel Healthcare Center, Qingdao, ChinaObjective(s): Heparin-binding hemagglutinin (HBHA), a mycobacterial cell surface protein, mediates adhesion to nonphagocytic cells and the dissemination of Mycobacterium tuberculosis (M. tuberculosis) from the site of primary infection. Superior expression systems are required to obtain abundant M. tuberculosis proteins for the purpose of diagnosing M. tuberculosis infection or for the immunization. Here, HBHA was expressed by Pichia pastoris (P. pastoris) GS115 strain , and the immunogenicity of HBHA was evaluated. Materials and Methods: The HBHA gene of M. tuberculosis was cloned into the pPIC9K plasmid, which was good for electroporation into P. pastoris GS115 strain. Unlabeled HBHA protein was purified using a Sepharose CL-6B column, and its expression was confirmed using anti-HBHA polyclonal antibody from mouse serum. We injected C57BL/6 mice with HBHA/ dimethyldioctadecylammonium/trehalose 6,6′-dibehenate (HBHA/DDA/TDB) to investigate the immunogenicity of this potential vaccine. Results: The results demonstrated that HBHA/DDA/TDB has the ability to induce high levels of HBHA-specific IgG antibody and its subclasses, as well as interferon-gamma, compared with injection of phosphate-buffered saline, DDA/TDB alone and Bacillus Calmette-Guérin (BCG) controls (Phttp://ijbms.mums.ac.ir/article_10107_84f37b7d3c2befe07e5c8149094f6015.pdfDDAHeparin-binding- hemagglutininMycobacteriumPichia pastoris GS115TuberculosisTDB |
| spellingShingle | Xindong Teng Xiaoguang Chen Ke Zhu Hefei Xu Immunogenicity of heparin-binding hemagglutinin expressed by Pichia pastoris GS115 strain Iranian Journal of Basic Medical Sciences DDA Heparin-binding- hemagglutinin Mycobacterium Pichia pastoris GS115 Tuberculosis TDB |
| title | Immunogenicity of heparin-binding hemagglutinin expressed by Pichia pastoris GS115 strain |
| title_full | Immunogenicity of heparin-binding hemagglutinin expressed by Pichia pastoris GS115 strain |
| title_fullStr | Immunogenicity of heparin-binding hemagglutinin expressed by Pichia pastoris GS115 strain |
| title_full_unstemmed | Immunogenicity of heparin-binding hemagglutinin expressed by Pichia pastoris GS115 strain |
| title_short | Immunogenicity of heparin-binding hemagglutinin expressed by Pichia pastoris GS115 strain |
| title_sort | immunogenicity of heparin binding hemagglutinin expressed by pichia pastoris gs115 strain |
| topic | DDA Heparin-binding- hemagglutinin Mycobacterium Pichia pastoris GS115 Tuberculosis TDB |
| url | http://ijbms.mums.ac.ir/article_10107_84f37b7d3c2befe07e5c8149094f6015.pdf |
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