Circular RNA‐mediated ceRNA network was identified in human lung adenocarcinoma by high‐throughput sequencing

Abstract Objectives Aberrantly expressed circular RNAs (circRNAs) have been detected in many types of tumors. Hence, they are currently investigated as candidate biomarkers for diagnostic and potential targets for therapy in cancers. The objective of this study was to assess the expression profile o...

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Main Authors: Yongyang Chen, Xiaoling Han, Xiaobi Huang, Honglian Zhou, Hui Yu, Lihui Wang, Zijian Liu, Baiyang Liu, Jian Huang, Yinghuan Xiong, Yang Shao, Dongqin Zhu, Zhu Liang, Zhixiong Yang, Wenmei Su
Format: Article
Language:English
Published: Wiley 2023-06-01
Series:Thoracic Cancer
Subjects:
Online Access:https://doi.org/10.1111/1759-7714.14884
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author Yongyang Chen
Xiaoling Han
Xiaobi Huang
Honglian Zhou
Hui Yu
Lihui Wang
Zijian Liu
Baiyang Liu
Jian Huang
Yinghuan Xiong
Jian Huang
Yang Shao
Dongqin Zhu
Zhu Liang
Zhixiong Yang
Wenmei Su
author_facet Yongyang Chen
Xiaoling Han
Xiaobi Huang
Honglian Zhou
Hui Yu
Lihui Wang
Zijian Liu
Baiyang Liu
Jian Huang
Yinghuan Xiong
Jian Huang
Yang Shao
Dongqin Zhu
Zhu Liang
Zhixiong Yang
Wenmei Su
author_sort Yongyang Chen
collection DOAJ
description Abstract Objectives Aberrantly expressed circular RNAs (circRNAs) have been detected in many types of tumors. Hence, they are currently investigated as candidate biomarkers for diagnostic and potential targets for therapy in cancers. The objective of this study was to assess the expression profile of circRNA in lung adenocarcinoma (LUAD). Methods This study included 14 pairs of postoperative lung adenocarcinoma specimens, including cancer tissues and matched adjacent tissues. Second‐generation sequencing was applied to the specimens to determine the circRNA expression in them among the 5242 distinct circRNAs detected. Results We identified a total of 18 significantly dysregulated circRNAs in the LUAD tissues: upregulation in four and downregulation in 14. ROC (The receiver operating characteristic curve) further suggested that hsa_circ_0120106, has_circ_0007342, has_circ_0005937, and circRNA_0000826 could potentially be used as biomarkers in the diagnosis of LUAD. Furthermore, study of the circRNA–microRNA (miRNA)–messenger RNA (mRNA) revealed interactions between the 18 dysregulated circRNA and several cancer‐related miRNAs. Finally, a further Kyoto Encyclopedia of Genes and Genomes analysis showed that the cell cycle phase transition, p53 signaling pathway, AMP‐activated protein kinase (AMPK) relative signaling pathway, and so on were key putative pathways in the process of LUAD. Conclusions These findings demonstrated the correlation between abnormality in circRNA expression and LUAD, which lays the foundation of making CircRNAs candidate biomarkers in the diagnosis of LUAD.
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spelling doaj.art-8bd185bc26bc4c51a3008839f7c649ac2023-06-02T01:27:25ZengWileyThoracic Cancer1759-77061759-77142023-06-0114161430143910.1111/1759-7714.14884Circular RNA‐mediated ceRNA network was identified in human lung adenocarcinoma by high‐throughput sequencingYongyang Chen0Xiaoling Han1Xiaobi Huang2Honglian Zhou3Hui Yu4Lihui Wang5Zijian Liu6Baiyang Liu7Jian Huang8Yinghuan Xiong9Jian Huang10Yang Shao11Dongqin Zhu12Zhu Liang13Zhixiong Yang14Wenmei Su15Department of Pulmonary Oncology Affiliated Hospital of Guangdong Medical University Zhanjiang ChinaSecond Faculty of Clinical Medicine Guangdong Medical University Zhanjiang ChinaDepartment of Pulmonary Oncology Affiliated Hospital of Guangdong Medical University Zhanjiang ChinaDepartment of Pulmonary Oncology Affiliated Hospital of Guangdong Medical University Zhanjiang ChinaDepartment of Pulmonary Oncology Affiliated Hospital of Guangdong Medical University Zhanjiang ChinaKey Laboratory of Longevity and Aging‐Related Diseases of Chinese Ministry of Education, Center for Translational Medicine & School of Preclinical Medicine Guangxi Medical University Nanning ChinaDepartment of Pulmonary Oncology Affiliated Hospital of Guangdong Medical University Zhanjiang ChinaDepartment of Pulmonary Oncology Affiliated Hospital of Guangdong Medical University Zhanjiang ChinaDepartment of Pulmonary Oncology Affiliated Hospital of Guangdong Medical University Zhanjiang ChinaDepartment of Pulmonary Oncology Affiliated Hospital of Guangdong Medical University Zhanjiang ChinaDepartment of Thoracic Surgery Maoming People's Hospital Maoming ChinaTechnical Department Nanjing Geneseeq Technology Inc. Nanjing ChinaTechnical Department Nanjing Geneseeq Technology Inc. Nanjing ChinaDepartment of Pulmonary Oncology Affiliated Hospital of Guangdong Medical University Zhanjiang ChinaDepartment of Pulmonary Oncology Affiliated Hospital of Guangdong Medical University Zhanjiang ChinaDepartment of Pulmonary Oncology Affiliated Hospital of Guangdong Medical University Zhanjiang ChinaAbstract Objectives Aberrantly expressed circular RNAs (circRNAs) have been detected in many types of tumors. Hence, they are currently investigated as candidate biomarkers for diagnostic and potential targets for therapy in cancers. The objective of this study was to assess the expression profile of circRNA in lung adenocarcinoma (LUAD). Methods This study included 14 pairs of postoperative lung adenocarcinoma specimens, including cancer tissues and matched adjacent tissues. Second‐generation sequencing was applied to the specimens to determine the circRNA expression in them among the 5242 distinct circRNAs detected. Results We identified a total of 18 significantly dysregulated circRNAs in the LUAD tissues: upregulation in four and downregulation in 14. ROC (The receiver operating characteristic curve) further suggested that hsa_circ_0120106, has_circ_0007342, has_circ_0005937, and circRNA_0000826 could potentially be used as biomarkers in the diagnosis of LUAD. Furthermore, study of the circRNA–microRNA (miRNA)–messenger RNA (mRNA) revealed interactions between the 18 dysregulated circRNA and several cancer‐related miRNAs. Finally, a further Kyoto Encyclopedia of Genes and Genomes analysis showed that the cell cycle phase transition, p53 signaling pathway, AMP‐activated protein kinase (AMPK) relative signaling pathway, and so on were key putative pathways in the process of LUAD. Conclusions These findings demonstrated the correlation between abnormality in circRNA expression and LUAD, which lays the foundation of making CircRNAs candidate biomarkers in the diagnosis of LUAD.https://doi.org/10.1111/1759-7714.14884circular RNAshigh‐throughput sequencinglung adenocarcinoma
spellingShingle Yongyang Chen
Xiaoling Han
Xiaobi Huang
Honglian Zhou
Hui Yu
Lihui Wang
Zijian Liu
Baiyang Liu
Jian Huang
Yinghuan Xiong
Jian Huang
Yang Shao
Dongqin Zhu
Zhu Liang
Zhixiong Yang
Wenmei Su
Circular RNA‐mediated ceRNA network was identified in human lung adenocarcinoma by high‐throughput sequencing
Thoracic Cancer
circular RNAs
high‐throughput sequencing
lung adenocarcinoma
title Circular RNA‐mediated ceRNA network was identified in human lung adenocarcinoma by high‐throughput sequencing
title_full Circular RNA‐mediated ceRNA network was identified in human lung adenocarcinoma by high‐throughput sequencing
title_fullStr Circular RNA‐mediated ceRNA network was identified in human lung adenocarcinoma by high‐throughput sequencing
title_full_unstemmed Circular RNA‐mediated ceRNA network was identified in human lung adenocarcinoma by high‐throughput sequencing
title_short Circular RNA‐mediated ceRNA network was identified in human lung adenocarcinoma by high‐throughput sequencing
title_sort circular rna mediated cerna network was identified in human lung adenocarcinoma by high throughput sequencing
topic circular RNAs
high‐throughput sequencing
lung adenocarcinoma
url https://doi.org/10.1111/1759-7714.14884
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