Comparison of the response of alternative oxidase and uncoupling proteins to bacterial elicitor induced oxidative burst.
Plant UCPs are proved to take part in the fine-tuning of mitochondrial ROS generation. It has emerged that mitochondrion can be an important early source of intracellular ROS during plant-pathogen interaction thus plant UCPs must also play key role in this redox fine-tuning during the early phase of...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2019-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0210592 |
| _version_ | 1819123735424139264 |
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| author | Ádám Czobor Péter Hajdinák Bence Németh Borbála Piros Áron Németh András Szarka |
| author_facet | Ádám Czobor Péter Hajdinák Bence Németh Borbála Piros Áron Németh András Szarka |
| author_sort | Ádám Czobor |
| collection | DOAJ |
| description | Plant UCPs are proved to take part in the fine-tuning of mitochondrial ROS generation. It has emerged that mitochondrion can be an important early source of intracellular ROS during plant-pathogen interaction thus plant UCPs must also play key role in this redox fine-tuning during the early phase of plant-pathogen interaction. On the contrary of this well-established assumption, the expression of plant UCPs and their activity has not been investigated in elicitor induced oxidative burst. Thus, the level of plant UCPs both at RNA and protein level and their activity was investigated and compared to AOX as a reference in Arabidopsis thaliana cells due to bacterial harpin treatments. Similar to the expression and activity of AOX, the transcript level of UCP4, UCP5 and the UCP activity increased due to harpin treatment and the consequential oxidative burst. The expression of UCP4 and UCP5 elevated 15-18-fold after 1 h of treatment, then the activity of UCP reached its maximal value at 4h of treatment. The quite rapid activation of UCP due to harpin treatment gives another possibility to fine tune the redox balance of plant cell, furthermore explains the earlier observed rapid decrease of mitochondrial membrane potential and consequent decrease of ATP synthesis after harpin treatment. |
| first_indexed | 2024-12-22T07:13:04Z |
| format | Article |
| id | doaj.art-8bd6288be58f4c4180ba46693d74b2f6 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T07:13:04Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-8bd6288be58f4c4180ba46693d74b2f62022-12-21T18:34:27ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01141e021059210.1371/journal.pone.0210592Comparison of the response of alternative oxidase and uncoupling proteins to bacterial elicitor induced oxidative burst.Ádám CzoborPéter HajdinákBence NémethBorbála PirosÁron NémethAndrás SzarkaPlant UCPs are proved to take part in the fine-tuning of mitochondrial ROS generation. It has emerged that mitochondrion can be an important early source of intracellular ROS during plant-pathogen interaction thus plant UCPs must also play key role in this redox fine-tuning during the early phase of plant-pathogen interaction. On the contrary of this well-established assumption, the expression of plant UCPs and their activity has not been investigated in elicitor induced oxidative burst. Thus, the level of plant UCPs both at RNA and protein level and their activity was investigated and compared to AOX as a reference in Arabidopsis thaliana cells due to bacterial harpin treatments. Similar to the expression and activity of AOX, the transcript level of UCP4, UCP5 and the UCP activity increased due to harpin treatment and the consequential oxidative burst. The expression of UCP4 and UCP5 elevated 15-18-fold after 1 h of treatment, then the activity of UCP reached its maximal value at 4h of treatment. The quite rapid activation of UCP due to harpin treatment gives another possibility to fine tune the redox balance of plant cell, furthermore explains the earlier observed rapid decrease of mitochondrial membrane potential and consequent decrease of ATP synthesis after harpin treatment.https://doi.org/10.1371/journal.pone.0210592 |
| spellingShingle | Ádám Czobor Péter Hajdinák Bence Németh Borbála Piros Áron Németh András Szarka Comparison of the response of alternative oxidase and uncoupling proteins to bacterial elicitor induced oxidative burst. PLoS ONE |
| title | Comparison of the response of alternative oxidase and uncoupling proteins to bacterial elicitor induced oxidative burst. |
| title_full | Comparison of the response of alternative oxidase and uncoupling proteins to bacterial elicitor induced oxidative burst. |
| title_fullStr | Comparison of the response of alternative oxidase and uncoupling proteins to bacterial elicitor induced oxidative burst. |
| title_full_unstemmed | Comparison of the response of alternative oxidase and uncoupling proteins to bacterial elicitor induced oxidative burst. |
| title_short | Comparison of the response of alternative oxidase and uncoupling proteins to bacterial elicitor induced oxidative burst. |
| title_sort | comparison of the response of alternative oxidase and uncoupling proteins to bacterial elicitor induced oxidative burst |
| url | https://doi.org/10.1371/journal.pone.0210592 |
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