Diversity of Pectobacterium strains by biochemical, physiological, and molecular characterization
Pectobacterium is a complex taxon of strains with diverse characteristics. It comprises several genera, including Erwinia, Brenneria, Pectobacterium, Dickeya, and Pantoea. Pectobacterium and Dickeya cause diseases in a wide range of plants, including potatoes, where they are causative agents of soft...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Uberlândia
2020-02-01
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| Series: | Bioscience Journal |
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| Online Access: | http://www.seer.ufu.br/index.php/biosciencejournal/article/view/46256 |
| _version_ | 1818742173764419584 |
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| author | Adelaide Siqueira Silva José Magno Queiroz Luz Nilvanira Donizete Tebaldi Tâmara Prado de Morais |
| author_facet | Adelaide Siqueira Silva José Magno Queiroz Luz Nilvanira Donizete Tebaldi Tâmara Prado de Morais |
| author_sort | Adelaide Siqueira Silva |
| collection | DOAJ |
| description | Pectobacterium is a complex taxon of strains with diverse characteristics. It comprises several genera, including Erwinia, Brenneria, Pectobacterium, Dickeya, and Pantoea. Pectobacterium and Dickeya cause diseases in a wide range of plants, including potatoes, where they are causative agents of soft rot in tubers and blackleg in field-grown plants. Characterizing Pectobacterium species allows for the analysis of the diversity of pectinolytic bacteria, which may support control strategies for plant bacterial diseases. The aim of this study was to perform biochemical, physiological, and molecular characterizations of Pectobacterium spp. from different sites and host plants. The isolated strains were characterized by the glucose fermentation test, Gram staining, catalase activity, oxidase activity, growth at 37 ºC, reducing substances from sucrose, phosphatase activity, indole production, acid production from different sources (sorbitol, melibiose, citrate, and lactose), pathogenicity in potato, and hypersensitivity reactions. Molecular characterization was performed with species-specific primers ECA1f/ECA2r and EXPCCF/EXPCCR, which identify P. atrosepticum and P. carotovorum subsp. carotovorum (Pcc), respectively, and with primers 1491f/L1RA/L1RG and Br1f/L1RA/L1RG that differentiate Pcc from Dickeya chrysanthemi and from P. carotovorum subsp. brasiliensis. The strains were identified as belonging to the genus Pectobacterium, though they did not fit the biochemical nor the molecular classification standards for subspecies differentiation, indicating significant diversity among the strains. |
| first_indexed | 2024-12-18T02:08:19Z |
| format | Article |
| id | doaj.art-8bd636b27a984b56bcb03212dd72d028 |
| institution | Directory Open Access Journal |
| issn | 1981-3163 |
| language | English |
| last_indexed | 2024-12-18T02:08:19Z |
| publishDate | 2020-02-01 |
| publisher | Universidade Federal de Uberlândia |
| record_format | Article |
| series | Bioscience Journal |
| spelling | doaj.art-8bd636b27a984b56bcb03212dd72d0282022-12-21T21:24:32ZengUniversidade Federal de UberlândiaBioscience Journal1981-31632020-02-0136210.14393/BJ-v36n2a2020-4625646256Diversity of Pectobacterium strains by biochemical, physiological, and molecular characterizationAdelaide Siqueira Silva0José Magno Queiroz Luz1Nilvanira Donizete Tebaldi2Tâmara Prado de Morais3Federal University of UberlândiaFederal University of UberlândiaFederal University of UberlândiaFederal University of UberlândiaPectobacterium is a complex taxon of strains with diverse characteristics. It comprises several genera, including Erwinia, Brenneria, Pectobacterium, Dickeya, and Pantoea. Pectobacterium and Dickeya cause diseases in a wide range of plants, including potatoes, where they are causative agents of soft rot in tubers and blackleg in field-grown plants. Characterizing Pectobacterium species allows for the analysis of the diversity of pectinolytic bacteria, which may support control strategies for plant bacterial diseases. The aim of this study was to perform biochemical, physiological, and molecular characterizations of Pectobacterium spp. from different sites and host plants. The isolated strains were characterized by the glucose fermentation test, Gram staining, catalase activity, oxidase activity, growth at 37 ºC, reducing substances from sucrose, phosphatase activity, indole production, acid production from different sources (sorbitol, melibiose, citrate, and lactose), pathogenicity in potato, and hypersensitivity reactions. Molecular characterization was performed with species-specific primers ECA1f/ECA2r and EXPCCF/EXPCCR, which identify P. atrosepticum and P. carotovorum subsp. carotovorum (Pcc), respectively, and with primers 1491f/L1RA/L1RG and Br1f/L1RA/L1RG that differentiate Pcc from Dickeya chrysanthemi and from P. carotovorum subsp. brasiliensis. The strains were identified as belonging to the genus Pectobacterium, though they did not fit the biochemical nor the molecular classification standards for subspecies differentiation, indicating significant diversity among the strains.http://www.seer.ufu.br/index.php/biosciencejournal/article/view/46256phytobacteriapotato blacklegpolymerase chain reactionsolanum tuberosum |
| spellingShingle | Adelaide Siqueira Silva José Magno Queiroz Luz Nilvanira Donizete Tebaldi Tâmara Prado de Morais Diversity of Pectobacterium strains by biochemical, physiological, and molecular characterization Bioscience Journal phytobacteria potato blackleg polymerase chain reaction solanum tuberosum |
| title | Diversity of Pectobacterium strains by biochemical, physiological, and molecular characterization |
| title_full | Diversity of Pectobacterium strains by biochemical, physiological, and molecular characterization |
| title_fullStr | Diversity of Pectobacterium strains by biochemical, physiological, and molecular characterization |
| title_full_unstemmed | Diversity of Pectobacterium strains by biochemical, physiological, and molecular characterization |
| title_short | Diversity of Pectobacterium strains by biochemical, physiological, and molecular characterization |
| title_sort | diversity of pectobacterium strains by biochemical physiological and molecular characterization |
| topic | phytobacteria potato blackleg polymerase chain reaction solanum tuberosum |
| url | http://www.seer.ufu.br/index.php/biosciencejournal/article/view/46256 |
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