Interaction of Fumonisin B1, <i>N</i>-Palmitoyl-Fumonisin B1, 5-<i>O</i>-Palmitoyl-Fumonisin B1, and Fumonisin B4 Mycotoxins with Human Serum Albumin and Their Toxic Impacts on Zebrafish Embryos
Fumonisins are frequent food contaminants. The high exposure to fumonisins can cause harmful effects in humans and animals. Fumonisin B1 (FB1) is the most typical member of this group; however, the occurrence of several other derivatives has been reported. Acylated metabolites of FB1 have also been...
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| Format: | Article |
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MDPI AG
2023-04-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/13/5/755 |
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| author | Zsolt Csenki Tibor Bartók Illés Bock Levente Horváth Beáta Lemli Balázs Zoltán Zsidó Cserne Angeli Csaba Hetényi István Szabó Béla Urbányi Melinda Kovács Miklós Poór |
| author_facet | Zsolt Csenki Tibor Bartók Illés Bock Levente Horváth Beáta Lemli Balázs Zoltán Zsidó Cserne Angeli Csaba Hetényi István Szabó Béla Urbányi Melinda Kovács Miklós Poór |
| author_sort | Zsolt Csenki |
| collection | DOAJ |
| description | Fumonisins are frequent food contaminants. The high exposure to fumonisins can cause harmful effects in humans and animals. Fumonisin B1 (FB1) is the most typical member of this group; however, the occurrence of several other derivatives has been reported. Acylated metabolites of FB1 have also been described as possible food contaminants, and the very limited data available suggest their significantly higher toxicity compared to FB1. Furthermore, the physicochemical and toxicokinetic properties (e.g., albumin binding) of acyl-FB1 derivatives may show large differences compared to the parent mycotoxin. Therefore, we tested the interactions of FB1, <i>N</i>-palmitoyl-FB1 (<i>N</i>-pal-FB1), 5-<i>O</i>-palmitoyl-FB1 (5-<i>O</i>-pal-FB1), and fumonisin B4 (FB4) with human serum albumin as well as the toxic effects of these mycotoxins on zebrafish embryos were examined. Based on our results, the most important observations and conclusions are the following: (1) FB1 and FB4 bind to albumin with low affinity, while palmitoyl-FB1 derivatives form highly stable complexes with the protein. (2) <i>N</i>-pal-FB1 and 5-<i>O</i>-pal-FB1 likely occupy more high-affinity binding sites on albumin. (3) Among the mycotoxins tested, <i>N</i>-pal-FB1 showed the most toxic effects on zebrafish, followed by 5-<i>O</i>-pal-FB1, FB4, and FB1. (4) Our study provides the first in vivo toxicity data regarding <i>N</i>-pal-FB1, 5-<i>O</i>-pal-FB1, and FB4. |
| first_indexed | 2024-03-11T03:54:52Z |
| format | Article |
| id | doaj.art-8bdb4cf7239546baaa6bd0e306cd3a6c |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-11T03:54:52Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-8bdb4cf7239546baaa6bd0e306cd3a6c2023-11-18T00:38:43ZengMDPI AGBiomolecules2218-273X2023-04-0113575510.3390/biom13050755Interaction of Fumonisin B1, <i>N</i>-Palmitoyl-Fumonisin B1, 5-<i>O</i>-Palmitoyl-Fumonisin B1, and Fumonisin B4 Mycotoxins with Human Serum Albumin and Their Toxic Impacts on Zebrafish EmbryosZsolt Csenki0Tibor Bartók1Illés Bock2Levente Horváth3Beáta Lemli4Balázs Zoltán Zsidó5Cserne Angeli6Csaba Hetényi7István Szabó8Béla Urbányi9Melinda Kovács10Miklós Poór11Department of Environmental Toxicology, Institute of Aquaculture and Environmental Safety, Hungarian University of Agriculture and Life Sciences, Páter Károly u. 1, H-2100 Gödöllő, HungaryFumizol Ltd., Kisfaludy u. 6/B, H-6725 Szeged, HungaryDepartment of Environmental Toxicology, Institute of Aquaculture and Environmental Safety, Hungarian University of Agriculture and Life Sciences, Páter Károly u. 1, H-2100 Gödöllő, HungaryFumizol Ltd., Kisfaludy u. 6/B, H-6725 Szeged, HungaryDepartment of Pharmacology, Faculty of Pharmacy, University of Pécs, Rókus u. 2, H-7624 Pécs, HungaryDepartment of Pharmacology and Pharmacotherapy, Pharmacoinformatics Unit, Medical School, University of Pécs, Szigeti út 12, H-7624 Pécs, HungaryInstitute of Physiology and Nutrition, Agriobiotechnology and Precision Breeding for Food Security National Laboratory, Hungarian University of Agriculture and Life Sciences, Guba Sándor út 40, H-7400 Kaposvár, HungaryDepartment of Pharmacology and Pharmacotherapy, Pharmacoinformatics Unit, Medical School, University of Pécs, Szigeti út 12, H-7624 Pécs, HungaryDepartment of Environmental Toxicology, Institute of Aquaculture and Environmental Safety, Hungarian University of Agriculture and Life Sciences, Páter Károly u. 1, H-2100 Gödöllő, HungaryDepartment of Aquaculture, Institute of Aquaculture and Environmental Safety, Hungarian University of Agriculture and Life Sciences, Páter Károly u. 1, H-2100 Gödöllő, HungaryInstitute of Physiology and Nutrition, Agriobiotechnology and Precision Breeding for Food Security National Laboratory, Hungarian University of Agriculture and Life Sciences, Guba Sándor út 40, H-7400 Kaposvár, HungaryDepartment of Pharmacology, Faculty of Pharmacy, University of Pécs, Rókus u. 2, H-7624 Pécs, HungaryFumonisins are frequent food contaminants. The high exposure to fumonisins can cause harmful effects in humans and animals. Fumonisin B1 (FB1) is the most typical member of this group; however, the occurrence of several other derivatives has been reported. Acylated metabolites of FB1 have also been described as possible food contaminants, and the very limited data available suggest their significantly higher toxicity compared to FB1. Furthermore, the physicochemical and toxicokinetic properties (e.g., albumin binding) of acyl-FB1 derivatives may show large differences compared to the parent mycotoxin. Therefore, we tested the interactions of FB1, <i>N</i>-palmitoyl-FB1 (<i>N</i>-pal-FB1), 5-<i>O</i>-palmitoyl-FB1 (5-<i>O</i>-pal-FB1), and fumonisin B4 (FB4) with human serum albumin as well as the toxic effects of these mycotoxins on zebrafish embryos were examined. Based on our results, the most important observations and conclusions are the following: (1) FB1 and FB4 bind to albumin with low affinity, while palmitoyl-FB1 derivatives form highly stable complexes with the protein. (2) <i>N</i>-pal-FB1 and 5-<i>O</i>-pal-FB1 likely occupy more high-affinity binding sites on albumin. (3) Among the mycotoxins tested, <i>N</i>-pal-FB1 showed the most toxic effects on zebrafish, followed by 5-<i>O</i>-pal-FB1, FB4, and FB1. (4) Our study provides the first in vivo toxicity data regarding <i>N</i>-pal-FB1, 5-<i>O</i>-pal-FB1, and FB4.https://www.mdpi.com/2218-273X/13/5/755fumonisin B1<i>N</i>-palmitoyl-fumonisin B15-<i>O</i>-palmitoyl-fumonisin B1fumonisin B4human serum albuminzebrafish embryos |
| spellingShingle | Zsolt Csenki Tibor Bartók Illés Bock Levente Horváth Beáta Lemli Balázs Zoltán Zsidó Cserne Angeli Csaba Hetényi István Szabó Béla Urbányi Melinda Kovács Miklós Poór Interaction of Fumonisin B1, <i>N</i>-Palmitoyl-Fumonisin B1, 5-<i>O</i>-Palmitoyl-Fumonisin B1, and Fumonisin B4 Mycotoxins with Human Serum Albumin and Their Toxic Impacts on Zebrafish Embryos Biomolecules fumonisin B1 <i>N</i>-palmitoyl-fumonisin B1 5-<i>O</i>-palmitoyl-fumonisin B1 fumonisin B4 human serum albumin zebrafish embryos |
| title | Interaction of Fumonisin B1, <i>N</i>-Palmitoyl-Fumonisin B1, 5-<i>O</i>-Palmitoyl-Fumonisin B1, and Fumonisin B4 Mycotoxins with Human Serum Albumin and Their Toxic Impacts on Zebrafish Embryos |
| title_full | Interaction of Fumonisin B1, <i>N</i>-Palmitoyl-Fumonisin B1, 5-<i>O</i>-Palmitoyl-Fumonisin B1, and Fumonisin B4 Mycotoxins with Human Serum Albumin and Their Toxic Impacts on Zebrafish Embryos |
| title_fullStr | Interaction of Fumonisin B1, <i>N</i>-Palmitoyl-Fumonisin B1, 5-<i>O</i>-Palmitoyl-Fumonisin B1, and Fumonisin B4 Mycotoxins with Human Serum Albumin and Their Toxic Impacts on Zebrafish Embryos |
| title_full_unstemmed | Interaction of Fumonisin B1, <i>N</i>-Palmitoyl-Fumonisin B1, 5-<i>O</i>-Palmitoyl-Fumonisin B1, and Fumonisin B4 Mycotoxins with Human Serum Albumin and Their Toxic Impacts on Zebrafish Embryos |
| title_short | Interaction of Fumonisin B1, <i>N</i>-Palmitoyl-Fumonisin B1, 5-<i>O</i>-Palmitoyl-Fumonisin B1, and Fumonisin B4 Mycotoxins with Human Serum Albumin and Their Toxic Impacts on Zebrafish Embryos |
| title_sort | interaction of fumonisin b1 i n i palmitoyl fumonisin b1 5 i o i palmitoyl fumonisin b1 and fumonisin b4 mycotoxins with human serum albumin and their toxic impacts on zebrafish embryos |
| topic | fumonisin B1 <i>N</i>-palmitoyl-fumonisin B1 5-<i>O</i>-palmitoyl-fumonisin B1 fumonisin B4 human serum albumin zebrafish embryos |
| url | https://www.mdpi.com/2218-273X/13/5/755 |
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