NT-CRISPR, combining natural transformation and CRISPR-Cas9 counterselection for markerless and scarless genome editing in Vibrio natriegens
Stukenberg et al. present NT-CRISPR, a method for performing genome editing in the marine bacterium Vibrio natriegens without using antibiotic resistance or other types of markers. This method combines V. natriegens’ capability for highly efficient natural transformation with an extremely efficient...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-03-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-022-03150-0 |
| _version_ | 1819177048065703936 |
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| author | Daniel Stukenberg Josef Hoff Anna Faber Anke Becker |
| author_facet | Daniel Stukenberg Josef Hoff Anna Faber Anke Becker |
| author_sort | Daniel Stukenberg |
| collection | DOAJ |
| description | Stukenberg et al. present NT-CRISPR, a method for performing genome editing in the marine bacterium Vibrio natriegens without using antibiotic resistance or other types of markers. This method combines V. natriegens’ capability for highly efficient natural transformation with an extremely efficient CRISPR-Cas9-based counterselection step for editing efficiencies of up to 100% and highly efficient simultaneous deletion of multiple sequences. |
| first_indexed | 2024-12-22T21:20:27Z |
| format | Article |
| id | doaj.art-8bdcd0e8b0fb4a848e9d48db67e234fd |
| institution | Directory Open Access Journal |
| issn | 2399-3642 |
| language | English |
| last_indexed | 2024-12-22T21:20:27Z |
| publishDate | 2022-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj.art-8bdcd0e8b0fb4a848e9d48db67e234fd2022-12-21T18:12:13ZengNature PortfolioCommunications Biology2399-36422022-03-015111310.1038/s42003-022-03150-0NT-CRISPR, combining natural transformation and CRISPR-Cas9 counterselection for markerless and scarless genome editing in Vibrio natriegensDaniel Stukenberg0Josef Hoff1Anna Faber2Anke Becker3Center for Synthetic Microbiology, Philipps-Universität MarburgCenter for Synthetic Microbiology, Philipps-Universität MarburgCenter for Synthetic Microbiology, Philipps-Universität MarburgCenter for Synthetic Microbiology, Philipps-Universität MarburgStukenberg et al. present NT-CRISPR, a method for performing genome editing in the marine bacterium Vibrio natriegens without using antibiotic resistance or other types of markers. This method combines V. natriegens’ capability for highly efficient natural transformation with an extremely efficient CRISPR-Cas9-based counterselection step for editing efficiencies of up to 100% and highly efficient simultaneous deletion of multiple sequences.https://doi.org/10.1038/s42003-022-03150-0 |
| spellingShingle | Daniel Stukenberg Josef Hoff Anna Faber Anke Becker NT-CRISPR, combining natural transformation and CRISPR-Cas9 counterselection for markerless and scarless genome editing in Vibrio natriegens Communications Biology |
| title | NT-CRISPR, combining natural transformation and CRISPR-Cas9 counterselection for markerless and scarless genome editing in Vibrio natriegens |
| title_full | NT-CRISPR, combining natural transformation and CRISPR-Cas9 counterselection for markerless and scarless genome editing in Vibrio natriegens |
| title_fullStr | NT-CRISPR, combining natural transformation and CRISPR-Cas9 counterselection for markerless and scarless genome editing in Vibrio natriegens |
| title_full_unstemmed | NT-CRISPR, combining natural transformation and CRISPR-Cas9 counterselection for markerless and scarless genome editing in Vibrio natriegens |
| title_short | NT-CRISPR, combining natural transformation and CRISPR-Cas9 counterselection for markerless and scarless genome editing in Vibrio natriegens |
| title_sort | nt crispr combining natural transformation and crispr cas9 counterselection for markerless and scarless genome editing in vibrio natriegens |
| url | https://doi.org/10.1038/s42003-022-03150-0 |
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