Assessment of different manufacturing techniques for the production of bioartificial scaffolds as soft organ transplant substitutes
Introduction: The problem of organs’ shortage for transplantation is widely known: different manufacturing techniques such as Solvent casting, Electrospinning and 3D Printing were considered to produce bioartificial scaffolds for tissue engineering purposes and possible transplantation substitutes....
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-06-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1186351/full |
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| author | Silvia Pisani Valeria Mauri Erika Negrello Simone Mauramati Gianluca Alaimo Ferdinando Auricchio Marco Benazzo Marco Benazzo Rossella Dorati Ida Genta Bice Conti Virginia Valeria Ferretti Annalisa De Silvestri Andrea Pietrabissa Andrea Pietrabissa Stefania Marconi Stefania Marconi |
| author_facet | Silvia Pisani Valeria Mauri Erika Negrello Simone Mauramati Gianluca Alaimo Ferdinando Auricchio Marco Benazzo Marco Benazzo Rossella Dorati Ida Genta Bice Conti Virginia Valeria Ferretti Annalisa De Silvestri Andrea Pietrabissa Andrea Pietrabissa Stefania Marconi Stefania Marconi |
| author_sort | Silvia Pisani |
| collection | DOAJ |
| description | Introduction: The problem of organs’ shortage for transplantation is widely known: different manufacturing techniques such as Solvent casting, Electrospinning and 3D Printing were considered to produce bioartificial scaffolds for tissue engineering purposes and possible transplantation substitutes. The advantages of manufacturing techniques’ combination to develop hybrid scaffolds with increased performing properties was also evaluated.Methods: Scaffolds were produced using poly-L-lactide-co-caprolactone (PLA-PCL) copolymer and characterized for their morphological, biological, and mechanical features.Results: Hybrid scaffolds showed the best properties in terms of viability (>100%) and cell adhesion. Furthermore, their mechanical properties were found to be comparable with the reference values for soft tissues (range 1–10 MPa).Discussion: The created hybrid scaffolds pave the way for the future development of more complex systems capable of supporting, from a morphological, mechanical, and biological standpoint, the physiological needs of the tissues/organs to be transplanted. |
| first_indexed | 2024-03-13T03:01:45Z |
| format | Article |
| id | doaj.art-8be114e5957242558b0090ac0e429677 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-03-13T03:01:45Z |
| publishDate | 2023-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-8be114e5957242558b0090ac0e4296772023-06-27T11:53:18ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-06-011110.3389/fbioe.2023.11863511186351Assessment of different manufacturing techniques for the production of bioartificial scaffolds as soft organ transplant substitutesSilvia Pisani0Valeria Mauri1Erika Negrello2Simone Mauramati3Gianluca Alaimo4Ferdinando Auricchio5Marco Benazzo6Marco Benazzo7Rossella Dorati8Ida Genta9Bice Conti10Virginia Valeria Ferretti11Annalisa De Silvestri12Andrea Pietrabissa13Andrea Pietrabissa14Stefania Marconi15Stefania Marconi16Department of Otorhinolaryngology, Fondazione IRCCS Policlinico San Matteo, Pavia, ItalySC General Surgery 2, Fondazione IRCCS Policlinico San Matteo, Pavia, ItalySC General Surgery 2, Fondazione IRCCS Policlinico San Matteo, Pavia, ItalyDepartment of Otorhinolaryngology, Fondazione IRCCS Policlinico San Matteo, Pavia, ItalyDepartment of Civil Engineering and Architecture, University of Pavia, Pavia, ItalyDepartment of Civil Engineering and Architecture, University of Pavia, Pavia, ItalyDepartment of Otorhinolaryngology, Fondazione IRCCS Policlinico San Matteo, Pavia, ItalyDepartment of Clinical, Surgical, Diagnostic and Pediatric Sciences, University of Pavia, Pavia, ItalyDepartment of Drug Sciences, University of Pavia, Pavia, ItalyDepartment of Drug Sciences, University of Pavia, Pavia, ItalyDepartment of Drug Sciences, University of Pavia, Pavia, ItalySSD Biostatistica e Clinical Trial Center, Fondazione IRCCS Policlinico San Matteo, Pavia, ItalySSD Biostatistica e Clinical Trial Center, Fondazione IRCCS Policlinico San Matteo, Pavia, ItalySC General Surgery 2, Fondazione IRCCS Policlinico San Matteo, Pavia, ItalyDepartment of Surgery, University of Pavia, Pavia, ItalyDepartment of Civil Engineering and Architecture, University of Pavia, Pavia, ItalyFondazione IRCCS Policlinico San Matteo, Pavia, ItalyIntroduction: The problem of organs’ shortage for transplantation is widely known: different manufacturing techniques such as Solvent casting, Electrospinning and 3D Printing were considered to produce bioartificial scaffolds for tissue engineering purposes and possible transplantation substitutes. The advantages of manufacturing techniques’ combination to develop hybrid scaffolds with increased performing properties was also evaluated.Methods: Scaffolds were produced using poly-L-lactide-co-caprolactone (PLA-PCL) copolymer and characterized for their morphological, biological, and mechanical features.Results: Hybrid scaffolds showed the best properties in terms of viability (>100%) and cell adhesion. Furthermore, their mechanical properties were found to be comparable with the reference values for soft tissues (range 1–10 MPa).Discussion: The created hybrid scaffolds pave the way for the future development of more complex systems capable of supporting, from a morphological, mechanical, and biological standpoint, the physiological needs of the tissues/organs to be transplanted.https://www.frontiersin.org/articles/10.3389/fbioe.2023.1186351/fullbioartificial scaffoldstissue engineering3D printingelectrospinningtransplantologyorgan transplant |
| spellingShingle | Silvia Pisani Valeria Mauri Erika Negrello Simone Mauramati Gianluca Alaimo Ferdinando Auricchio Marco Benazzo Marco Benazzo Rossella Dorati Ida Genta Bice Conti Virginia Valeria Ferretti Annalisa De Silvestri Andrea Pietrabissa Andrea Pietrabissa Stefania Marconi Stefania Marconi Assessment of different manufacturing techniques for the production of bioartificial scaffolds as soft organ transplant substitutes Frontiers in Bioengineering and Biotechnology bioartificial scaffolds tissue engineering 3D printing electrospinning transplantology organ transplant |
| title | Assessment of different manufacturing techniques for the production of bioartificial scaffolds as soft organ transplant substitutes |
| title_full | Assessment of different manufacturing techniques for the production of bioartificial scaffolds as soft organ transplant substitutes |
| title_fullStr | Assessment of different manufacturing techniques for the production of bioartificial scaffolds as soft organ transplant substitutes |
| title_full_unstemmed | Assessment of different manufacturing techniques for the production of bioartificial scaffolds as soft organ transplant substitutes |
| title_short | Assessment of different manufacturing techniques for the production of bioartificial scaffolds as soft organ transplant substitutes |
| title_sort | assessment of different manufacturing techniques for the production of bioartificial scaffolds as soft organ transplant substitutes |
| topic | bioartificial scaffolds tissue engineering 3D printing electrospinning transplantology organ transplant |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1186351/full |
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