Phylogeny of <i>PmCCD</i> Gene Family and Expression Analysis of Flower Coloration and Stress Response in <i>Prunus mume</i>
The <i>CCD</i> gene family plays a crucial role in the cleavage of carotenoids, converting them into apocarotenoids. This process not only impacts the physiology and development of plants but also enhances their tolerance toward different stresses. However, the character of the <i>...
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MDPI AG
2023-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/18/13950 |
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| author | Aiqin Ding Fei Bao Wenhui Cheng Tangren Cheng Qixiang Zhang |
| author_facet | Aiqin Ding Fei Bao Wenhui Cheng Tangren Cheng Qixiang Zhang |
| author_sort | Aiqin Ding |
| collection | DOAJ |
| description | The <i>CCD</i> gene family plays a crucial role in the cleavage of carotenoids, converting them into apocarotenoids. This process not only impacts the physiology and development of plants but also enhances their tolerance toward different stresses. However, the character of the <i>PmCCD</i> gene family and its role in ornamental woody <i>Prunus mume</i> remain unclear. Here, ten non-redundant <i>PmCCD</i> genes were identified from the <i>P. mume</i> genome, and their physicochemical characteristics were predicted. According to the phylogenetic tree, PmCCD proteins were classified into six subfamilies: CCD1, CCD4, CCD7, CCD8, NCED and CCD-like. The same subfamily possessed similar gene structural patterns and numbers of conserved motifs. Ten <i>PmCCD</i> genes were concentrated on three chromosomes. <i>PmCCD</i> genes exhibited interspecific collinearity with <i>P. armeniaca</i> and <i>P. persica</i>. Additionally, <i>PmCCD</i> genes had obvious specificity in different tissues and varieties. Compared with white-flowered ‘ZLE’, <i>PmCCD1</i> and <i>PmCCD4</i> genes were low-expressed in ‘HJH’ with yellow petals, which suggested <i>PmCCD1</i> and <i>PmCCD4</i> might be related to the formation of yellow flowers in <i>P. mume</i>. Nine <i>PmCCD</i> genes could respond to NaCl or PEG treatments. These genes might play a crucial role in salt and drought resistance in <i>P. mume</i>. Moreover, PmVAR3 and PmSAT3/5 interacted with PmCCD4 protein in yeast and tobacco leaf cells. This study laid a foundation for exploring the role of the <i>PmCCD</i> gene family in flower coloration and stress response in <i>P. mume</i>. |
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| spelling | doaj.art-8be40763c7a84034866635b51b7392c92023-11-19T11:05:44ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-09-0124181395010.3390/ijms241813950Phylogeny of <i>PmCCD</i> Gene Family and Expression Analysis of Flower Coloration and Stress Response in <i>Prunus mume</i>Aiqin Ding0Fei Bao1Wenhui Cheng2Tangren Cheng3Qixiang Zhang4Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, National Engineering Research Center for Floriculture, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, ChinaBeijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, National Engineering Research Center for Floriculture, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, ChinaBeijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, National Engineering Research Center for Floriculture, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, ChinaBeijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, National Engineering Research Center for Floriculture, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, ChinaBeijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, National Engineering Research Center for Floriculture, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, ChinaThe <i>CCD</i> gene family plays a crucial role in the cleavage of carotenoids, converting them into apocarotenoids. This process not only impacts the physiology and development of plants but also enhances their tolerance toward different stresses. However, the character of the <i>PmCCD</i> gene family and its role in ornamental woody <i>Prunus mume</i> remain unclear. Here, ten non-redundant <i>PmCCD</i> genes were identified from the <i>P. mume</i> genome, and their physicochemical characteristics were predicted. According to the phylogenetic tree, PmCCD proteins were classified into six subfamilies: CCD1, CCD4, CCD7, CCD8, NCED and CCD-like. The same subfamily possessed similar gene structural patterns and numbers of conserved motifs. Ten <i>PmCCD</i> genes were concentrated on three chromosomes. <i>PmCCD</i> genes exhibited interspecific collinearity with <i>P. armeniaca</i> and <i>P. persica</i>. Additionally, <i>PmCCD</i> genes had obvious specificity in different tissues and varieties. Compared with white-flowered ‘ZLE’, <i>PmCCD1</i> and <i>PmCCD4</i> genes were low-expressed in ‘HJH’ with yellow petals, which suggested <i>PmCCD1</i> and <i>PmCCD4</i> might be related to the formation of yellow flowers in <i>P. mume</i>. Nine <i>PmCCD</i> genes could respond to NaCl or PEG treatments. These genes might play a crucial role in salt and drought resistance in <i>P. mume</i>. Moreover, PmVAR3 and PmSAT3/5 interacted with PmCCD4 protein in yeast and tobacco leaf cells. This study laid a foundation for exploring the role of the <i>PmCCD</i> gene family in flower coloration and stress response in <i>P. mume</i>.https://www.mdpi.com/1422-0067/24/18/13950<i>Prunus mume</i><i>PmCCDs</i>flower colorationstress responseexpression patternprotein interaction |
| spellingShingle | Aiqin Ding Fei Bao Wenhui Cheng Tangren Cheng Qixiang Zhang Phylogeny of <i>PmCCD</i> Gene Family and Expression Analysis of Flower Coloration and Stress Response in <i>Prunus mume</i> International Journal of Molecular Sciences <i>Prunus mume</i> <i>PmCCDs</i> flower coloration stress response expression pattern protein interaction |
| title | Phylogeny of <i>PmCCD</i> Gene Family and Expression Analysis of Flower Coloration and Stress Response in <i>Prunus mume</i> |
| title_full | Phylogeny of <i>PmCCD</i> Gene Family and Expression Analysis of Flower Coloration and Stress Response in <i>Prunus mume</i> |
| title_fullStr | Phylogeny of <i>PmCCD</i> Gene Family and Expression Analysis of Flower Coloration and Stress Response in <i>Prunus mume</i> |
| title_full_unstemmed | Phylogeny of <i>PmCCD</i> Gene Family and Expression Analysis of Flower Coloration and Stress Response in <i>Prunus mume</i> |
| title_short | Phylogeny of <i>PmCCD</i> Gene Family and Expression Analysis of Flower Coloration and Stress Response in <i>Prunus mume</i> |
| title_sort | phylogeny of i pmccd i gene family and expression analysis of flower coloration and stress response in i prunus mume i |
| topic | <i>Prunus mume</i> <i>PmCCDs</i> flower coloration stress response expression pattern protein interaction |
| url | https://www.mdpi.com/1422-0067/24/18/13950 |
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