ZBED6 modulates the transcription of myogenic genes in mouse myoblast cells.
ZBED6 is a recently discovered transcription factor, unique to placental mammals, that has evolved from a domesticated DNA transposon. It acts as a repressor at the IGF2 locus. Here we show that ZBED6 acts as a transcriptional modulator in mouse myoblast cells, where more than 700 genes were differe...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3979763?pdf=render |
| _version_ | 1818043645763256320 |
|---|---|
| author | Lin Jiang Ola Wallerman Shady Younis Carl-Johan Rubin Elizabeth R Gilbert Elisabeth Sundström Awaisa Ghazal Xiaolan Zhang Li Wang Tarjei S Mikkelsen Göran Andersson Leif Andersson |
| author_facet | Lin Jiang Ola Wallerman Shady Younis Carl-Johan Rubin Elizabeth R Gilbert Elisabeth Sundström Awaisa Ghazal Xiaolan Zhang Li Wang Tarjei S Mikkelsen Göran Andersson Leif Andersson |
| author_sort | Lin Jiang |
| collection | DOAJ |
| description | ZBED6 is a recently discovered transcription factor, unique to placental mammals, that has evolved from a domesticated DNA transposon. It acts as a repressor at the IGF2 locus. Here we show that ZBED6 acts as a transcriptional modulator in mouse myoblast cells, where more than 700 genes were differentially expressed after Zbed6-silencing. The most significantly enriched GO term was muscle protein and contractile fiber, which was consistent with increased myotube formation. Twenty small nucleolar RNAs all showed increased expression after Zbed6-silencing. The co-localization of histone marks and ZBED6 binding sites and the effect of Zbed6-silencing on distribution of histone marks was evaluated by ChIP-seq analysis. There was a strong association between ZBED6 binding sites and the H3K4me3, H3K4me2 and H3K27ac modifications, which are usually found at active promoters, but no association with the repressive mark H3K27me3. Zbed6-silencing led to increased enrichment of active marks at myogenic genes, in agreement with the RNA-seq findings. We propose that ZBED6 preferentially binds to active promoters and modulates transcriptional activity without recruiting repressive histone modifications. |
| first_indexed | 2024-12-10T09:05:31Z |
| format | Article |
| id | doaj.art-8be74448d56b4cd8b456efec2dac58e5 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T09:05:31Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-8be74448d56b4cd8b456efec2dac58e52022-12-22T01:55:10ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0194e9418710.1371/journal.pone.0094187ZBED6 modulates the transcription of myogenic genes in mouse myoblast cells.Lin JiangOla WallermanShady YounisCarl-Johan RubinElizabeth R GilbertElisabeth SundströmAwaisa GhazalXiaolan ZhangLi WangTarjei S MikkelsenGöran AnderssonLeif AnderssonZBED6 is a recently discovered transcription factor, unique to placental mammals, that has evolved from a domesticated DNA transposon. It acts as a repressor at the IGF2 locus. Here we show that ZBED6 acts as a transcriptional modulator in mouse myoblast cells, where more than 700 genes were differentially expressed after Zbed6-silencing. The most significantly enriched GO term was muscle protein and contractile fiber, which was consistent with increased myotube formation. Twenty small nucleolar RNAs all showed increased expression after Zbed6-silencing. The co-localization of histone marks and ZBED6 binding sites and the effect of Zbed6-silencing on distribution of histone marks was evaluated by ChIP-seq analysis. There was a strong association between ZBED6 binding sites and the H3K4me3, H3K4me2 and H3K27ac modifications, which are usually found at active promoters, but no association with the repressive mark H3K27me3. Zbed6-silencing led to increased enrichment of active marks at myogenic genes, in agreement with the RNA-seq findings. We propose that ZBED6 preferentially binds to active promoters and modulates transcriptional activity without recruiting repressive histone modifications.http://europepmc.org/articles/PMC3979763?pdf=render |
| spellingShingle | Lin Jiang Ola Wallerman Shady Younis Carl-Johan Rubin Elizabeth R Gilbert Elisabeth Sundström Awaisa Ghazal Xiaolan Zhang Li Wang Tarjei S Mikkelsen Göran Andersson Leif Andersson ZBED6 modulates the transcription of myogenic genes in mouse myoblast cells. PLoS ONE |
| title | ZBED6 modulates the transcription of myogenic genes in mouse myoblast cells. |
| title_full | ZBED6 modulates the transcription of myogenic genes in mouse myoblast cells. |
| title_fullStr | ZBED6 modulates the transcription of myogenic genes in mouse myoblast cells. |
| title_full_unstemmed | ZBED6 modulates the transcription of myogenic genes in mouse myoblast cells. |
| title_short | ZBED6 modulates the transcription of myogenic genes in mouse myoblast cells. |
| title_sort | zbed6 modulates the transcription of myogenic genes in mouse myoblast cells |
| url | http://europepmc.org/articles/PMC3979763?pdf=render |
| work_keys_str_mv | AT linjiang zbed6modulatesthetranscriptionofmyogenicgenesinmousemyoblastcells AT olawallerman zbed6modulatesthetranscriptionofmyogenicgenesinmousemyoblastcells AT shadyyounis zbed6modulatesthetranscriptionofmyogenicgenesinmousemyoblastcells AT carljohanrubin zbed6modulatesthetranscriptionofmyogenicgenesinmousemyoblastcells AT elizabethrgilbert zbed6modulatesthetranscriptionofmyogenicgenesinmousemyoblastcells AT elisabethsundstrom zbed6modulatesthetranscriptionofmyogenicgenesinmousemyoblastcells AT awaisaghazal zbed6modulatesthetranscriptionofmyogenicgenesinmousemyoblastcells AT xiaolanzhang zbed6modulatesthetranscriptionofmyogenicgenesinmousemyoblastcells AT liwang zbed6modulatesthetranscriptionofmyogenicgenesinmousemyoblastcells AT tarjeismikkelsen zbed6modulatesthetranscriptionofmyogenicgenesinmousemyoblastcells AT goranandersson zbed6modulatesthetranscriptionofmyogenicgenesinmousemyoblastcells AT leifandersson zbed6modulatesthetranscriptionofmyogenicgenesinmousemyoblastcells |