Degradation Kinetics of Lignocellulolytic Enzymes in a Biogas Reactor Using Quantitative Mass Spectrometry
The supplementation of lignocellulose-degrading enzymes can be used to enhance the performance of biogas production in industrial biogas plants. Since the structural stability of these enzyme preparations is essential for efficient application, reliable methods for the assessment of enzyme stability...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/9/1/67 |
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| author | Jan Küchler Katharina Willenbücher Elisabeth Reiß Lea Nuß Marius Conrady Patrice Ramm Ulrike Schimpf Udo Reichl Ulrich Szewzyk Dirk Benndorf |
| author_facet | Jan Küchler Katharina Willenbücher Elisabeth Reiß Lea Nuß Marius Conrady Patrice Ramm Ulrike Schimpf Udo Reichl Ulrich Szewzyk Dirk Benndorf |
| author_sort | Jan Küchler |
| collection | DOAJ |
| description | The supplementation of lignocellulose-degrading enzymes can be used to enhance the performance of biogas production in industrial biogas plants. Since the structural stability of these enzyme preparations is essential for efficient application, reliable methods for the assessment of enzyme stability are crucial. Here, a mass-spectrometric-based assay was established to monitor the structural stability of enzymes, i.e., the structural integrity of these proteins, in anaerobic digestion (AD). The analysis of extracts of <i>Lentinula edodes</i> revealed the rapid degradation of lignocellulose-degrading enzymes, with an approximate half-life of 1.5 h. The observed low structural stability of lignocellulose-degrading enzymes in AD corresponded with previous results obtained for biogas content. The established workflow can be easily adapted for the monitoring of other enzyme formulations and provides a platform for evaluating the effects of enzyme additions in AD, together with a characterization of the biochemical methane potential used in order to determine the biodegradability of organic substrates. |
| first_indexed | 2024-03-09T12:47:03Z |
| format | Article |
| id | doaj.art-8becbfc3b4744b4f87a96f669c97dfae |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-09T12:47:03Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-8becbfc3b4744b4f87a96f669c97dfae2023-11-30T22:11:02ZengMDPI AGFermentation2311-56372023-01-01916710.3390/fermentation9010067Degradation Kinetics of Lignocellulolytic Enzymes in a Biogas Reactor Using Quantitative Mass SpectrometryJan Küchler0Katharina Willenbücher1Elisabeth Reiß2Lea Nuß3Marius Conrady4Patrice Ramm5Ulrike Schimpf6Udo Reichl7Ulrich Szewzyk8Dirk Benndorf9Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, 39106 Magdeburg, GermanySystem Microbiology, Department Bioengineering, Leibniz Institute for Agricultural Engineering and Bioeconomy (ATB), Max-Eyth-Allee 100, 14469 Potsdam, GermanyBioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, 39106 Magdeburg, GermanyBioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, 39106 Magdeburg, GermanyInstitute of Agricultural and Urban Ecological Projects, Berlin Humboldt University (IASP), Philippstr. 13, 10115 Berlin, GermanyInstitute of Agricultural and Urban Ecological Projects, Berlin Humboldt University (IASP), Philippstr. 13, 10115 Berlin, GermanyDivision of Glycoscience, Department of Chemistry, School of Engineering Science in Chemistry, Biotechnology and Health (CBH), Alba Nova University Center, Royal Institute of Technology (KTH), Roslagstullsbacken 21, 10691 Stockholm, SwedenBioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, 39106 Magdeburg, GermanyEnvironmental Microbiology, Institute of Environmental Technology, Faculty of Process Sciences, Technische Universität Berlin, Ernst-Reuter-Platz 1, 10587 Berlin, GermanyBioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, 39106 Magdeburg, GermanyThe supplementation of lignocellulose-degrading enzymes can be used to enhance the performance of biogas production in industrial biogas plants. Since the structural stability of these enzyme preparations is essential for efficient application, reliable methods for the assessment of enzyme stability are crucial. Here, a mass-spectrometric-based assay was established to monitor the structural stability of enzymes, i.e., the structural integrity of these proteins, in anaerobic digestion (AD). The analysis of extracts of <i>Lentinula edodes</i> revealed the rapid degradation of lignocellulose-degrading enzymes, with an approximate half-life of 1.5 h. The observed low structural stability of lignocellulose-degrading enzymes in AD corresponded with previous results obtained for biogas content. The established workflow can be easily adapted for the monitoring of other enzyme formulations and provides a platform for evaluating the effects of enzyme additions in AD, together with a characterization of the biochemical methane potential used in order to determine the biodegradability of organic substrates.https://www.mdpi.com/2311-5637/9/1/67mass spectrometrybiogas productionfungal enzymeslignocellulose conversion<i>Lentinula edodes</i> |
| spellingShingle | Jan Küchler Katharina Willenbücher Elisabeth Reiß Lea Nuß Marius Conrady Patrice Ramm Ulrike Schimpf Udo Reichl Ulrich Szewzyk Dirk Benndorf Degradation Kinetics of Lignocellulolytic Enzymes in a Biogas Reactor Using Quantitative Mass Spectrometry Fermentation mass spectrometry biogas production fungal enzymes lignocellulose conversion <i>Lentinula edodes</i> |
| title | Degradation Kinetics of Lignocellulolytic Enzymes in a Biogas Reactor Using Quantitative Mass Spectrometry |
| title_full | Degradation Kinetics of Lignocellulolytic Enzymes in a Biogas Reactor Using Quantitative Mass Spectrometry |
| title_fullStr | Degradation Kinetics of Lignocellulolytic Enzymes in a Biogas Reactor Using Quantitative Mass Spectrometry |
| title_full_unstemmed | Degradation Kinetics of Lignocellulolytic Enzymes in a Biogas Reactor Using Quantitative Mass Spectrometry |
| title_short | Degradation Kinetics of Lignocellulolytic Enzymes in a Biogas Reactor Using Quantitative Mass Spectrometry |
| title_sort | degradation kinetics of lignocellulolytic enzymes in a biogas reactor using quantitative mass spectrometry |
| topic | mass spectrometry biogas production fungal enzymes lignocellulose conversion <i>Lentinula edodes</i> |
| url | https://www.mdpi.com/2311-5637/9/1/67 |
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