Effect of Puerarin Regulated mTOR Signaling Pathway in Experimental Liver Injury
It is known that excessive hepatocellular apoptosis is a typical characteristic of hepatic disease, and is regulated by the mammalian target of rapamycin (mTOR) signaling pathway. As the main active component of Kudzu (Pueraria lobata) roots, which is frequently used to treat hepatic diseases, Puera...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-10-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphar.2018.01165/full |
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| author | Bu-Gao Zhou Hai-Mei Zhao Xiu-Yun Lu Wen Zhou Fu-Chun Liu Xue-Ke Liu Duan-Yong Liu Duan-Yong Liu |
| author_facet | Bu-Gao Zhou Hai-Mei Zhao Xiu-Yun Lu Wen Zhou Fu-Chun Liu Xue-Ke Liu Duan-Yong Liu Duan-Yong Liu |
| author_sort | Bu-Gao Zhou |
| collection | DOAJ |
| description | It is known that excessive hepatocellular apoptosis is a typical characteristic of hepatic disease, and is regulated by the mammalian target of rapamycin (mTOR) signaling pathway. As the main active component of Kudzu (Pueraria lobata) roots, which is frequently used to treat hepatic diseases, Puerarin (Pue) has been reported to alleviate and protect against hepatic injury. However, it is unclear whether Pue can inhibit mTOR signaling to prevent excessive apoptosis in the treatment of hepatic diseases. In the present study, Pue effectively ameliorated pathological injury of the liver, decreased serum enzyme (ALT, AST, γ-GT, AKP, DBIL, and TBIL) levels, regulated the balance between pro-inflammatory (TNF-α, IL-1β, IL-4, IL-6, and TGF-β1) and anti-inflammatory cytokines (IL-10), restored the cell cycle and inhibited hepatocellular apoptosis and caspase-3 expression in rats with liver injury induced by 2-AAF/PH. Pue inhibited p-mTOR, p-AKT and Raptor activity, and increased Rictor expression in the liver tissues of rats with experimental liver injury. These results indicated that Pue effectively regulated the activation of mTOR signaling pathway in the therapeutic and prophylactic process of Pue on experimental liver injury. |
| first_indexed | 2024-12-11T10:17:58Z |
| format | Article |
| id | doaj.art-8bed7bdd22924da1a09e86b750b3e367 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-11T10:17:58Z |
| publishDate | 2018-10-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Pharmacology |
| spelling | doaj.art-8bed7bdd22924da1a09e86b750b3e3672022-12-22T01:11:33ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122018-10-01910.3389/fphar.2018.01165417631Effect of Puerarin Regulated mTOR Signaling Pathway in Experimental Liver InjuryBu-Gao Zhou0Hai-Mei Zhao1Xiu-Yun Lu2Wen Zhou3Fu-Chun Liu4Xue-Ke Liu5Duan-Yong Liu6Duan-Yong Liu7Science and Technology College, Jiangxi University of Traditional Chinese Medicine, Nanchang, ChinaSchool of Basic Medical Sciences, Jiangxi University of Traditional Chinese Medicine, Nanchang, ChinaScience and Technology College, Jiangxi University of Traditional Chinese Medicine, Nanchang, ChinaScience and Technology College, Jiangxi University of Traditional Chinese Medicine, Nanchang, ChinaDepartment of Postgraduate, Jiangxi University of Traditional Chinese Medicine, Nanchang, ChinaDepartment of Postgraduate, Jiangxi University of Traditional Chinese Medicine, Nanchang, ChinaScience and Technology College, Jiangxi University of Traditional Chinese Medicine, Nanchang, ChinaKey Laboratory of Pharmacology of Traditional Chinese Medicine in Jiangxi, Nanchang, ChinaIt is known that excessive hepatocellular apoptosis is a typical characteristic of hepatic disease, and is regulated by the mammalian target of rapamycin (mTOR) signaling pathway. As the main active component of Kudzu (Pueraria lobata) roots, which is frequently used to treat hepatic diseases, Puerarin (Pue) has been reported to alleviate and protect against hepatic injury. However, it is unclear whether Pue can inhibit mTOR signaling to prevent excessive apoptosis in the treatment of hepatic diseases. In the present study, Pue effectively ameliorated pathological injury of the liver, decreased serum enzyme (ALT, AST, γ-GT, AKP, DBIL, and TBIL) levels, regulated the balance between pro-inflammatory (TNF-α, IL-1β, IL-4, IL-6, and TGF-β1) and anti-inflammatory cytokines (IL-10), restored the cell cycle and inhibited hepatocellular apoptosis and caspase-3 expression in rats with liver injury induced by 2-AAF/PH. Pue inhibited p-mTOR, p-AKT and Raptor activity, and increased Rictor expression in the liver tissues of rats with experimental liver injury. These results indicated that Pue effectively regulated the activation of mTOR signaling pathway in the therapeutic and prophylactic process of Pue on experimental liver injury.https://www.frontiersin.org/article/10.3389/fphar.2018.01165/fullpuerarinliver injurymTOR signaling pathwayhepatoprotective effectapoptosis |
| spellingShingle | Bu-Gao Zhou Hai-Mei Zhao Xiu-Yun Lu Wen Zhou Fu-Chun Liu Xue-Ke Liu Duan-Yong Liu Duan-Yong Liu Effect of Puerarin Regulated mTOR Signaling Pathway in Experimental Liver Injury Frontiers in Pharmacology puerarin liver injury mTOR signaling pathway hepatoprotective effect apoptosis |
| title | Effect of Puerarin Regulated mTOR Signaling Pathway in Experimental Liver Injury |
| title_full | Effect of Puerarin Regulated mTOR Signaling Pathway in Experimental Liver Injury |
| title_fullStr | Effect of Puerarin Regulated mTOR Signaling Pathway in Experimental Liver Injury |
| title_full_unstemmed | Effect of Puerarin Regulated mTOR Signaling Pathway in Experimental Liver Injury |
| title_short | Effect of Puerarin Regulated mTOR Signaling Pathway in Experimental Liver Injury |
| title_sort | effect of puerarin regulated mtor signaling pathway in experimental liver injury |
| topic | puerarin liver injury mTOR signaling pathway hepatoprotective effect apoptosis |
| url | https://www.frontiersin.org/article/10.3389/fphar.2018.01165/full |
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