Resveratrol Alleviates Acute <i>Campylobacter jejuni</i> Induced Enterocolitis in a Preclinical Murine Intervention Study
The polyphenolic compound resveratrol has been shown to exert health-beneficial properties. Given globally emerging <i>Campylobacter</i> infections in humans, we addressed potential anti-pathogenic, immuno-modulatory and intestinal epithelial barrier preserving properties of synthetic re...
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MDPI AG
2020-11-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/12/1858 |
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| author | Markus M. Heimesaat Soraya Mousavi Ulrike Escher Fábia Daniela Lobo de Sá Elisa Peh Jörg-Dieter Schulzke Sophie Kittler Roland Bücker Stefan Bereswill |
| author_facet | Markus M. Heimesaat Soraya Mousavi Ulrike Escher Fábia Daniela Lobo de Sá Elisa Peh Jörg-Dieter Schulzke Sophie Kittler Roland Bücker Stefan Bereswill |
| author_sort | Markus M. Heimesaat |
| collection | DOAJ |
| description | The polyphenolic compound resveratrol has been shown to exert health-beneficial properties. Given globally emerging <i>Campylobacter</i> infections in humans, we addressed potential anti-pathogenic, immuno-modulatory and intestinal epithelial barrier preserving properties of synthetic resveratrol in the present preclinical intervention study applying a murine acute campylobacteriosis model. Two days following peroral <i>C. jejuni</i> infection, secondary abiotic IL-10<sup>−/−</sup> mice were either subjected to resveratrol or placebo via the drinking water. Whereas placebo mice suffered from acute enterocolitis at day 6 post-infection, resveratrol treatment did not only lead to improved clinical conditions, but also to less pronounced colonic epithelial apoptosis as compared to placebo application. Furthermore, <i>C. jejuni</i> induced innate and adaptive immune cell responses were dampened in the large intestines upon resveratrol challenge and accompanied by less colonic nitric oxide secretion in the resveratrol versus the placebo cohort. Functional analyses revealed that resveratrol treatment could effectively rescue colonic epithelial barrier function in <i>C. jejuni</i> infected mice. Strikingly, the disease-alleviating effects of resveratrol could additionally be found in extra-intestinal and also systemic compartments at day 6 post-infection. For the first time, our current preclinical intervention study provides evidence that peroral resveratrol treatment exerts potent disease-alleviating effects during acute experimental campylobacteriosis. |
| first_indexed | 2024-03-10T14:35:07Z |
| format | Article |
| id | doaj.art-8bf6d520533d4f328c6649e7237cfc2c |
| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-10T14:35:07Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-8bf6d520533d4f328c6649e7237cfc2c2023-11-20T22:18:09ZengMDPI AGMicroorganisms2076-26072020-11-01812185810.3390/microorganisms8121858Resveratrol Alleviates Acute <i>Campylobacter jejuni</i> Induced Enterocolitis in a Preclinical Murine Intervention StudyMarkus M. Heimesaat0Soraya Mousavi1Ulrike Escher2Fábia Daniela Lobo de Sá3Elisa Peh4Jörg-Dieter Schulzke5Sophie Kittler6Roland Bücker7Stefan Bereswill8Institute of Microbiology, Infectious Diseases and Immunology, Charité—University Medicine Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 12203 Berlin, GermanyInstitute of Microbiology, Infectious Diseases and Immunology, Charité—University Medicine Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 12203 Berlin, GermanyInstitute of Microbiology, Infectious Diseases and Immunology, Charité—University Medicine Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 12203 Berlin, GermanyInstitute of Clinical Physiology, Department of Gastroenterology, Infectious Diseases and Rheumatology, Charité—Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 12203 Berlin, GermanyInstitute for Food Quality and Food Safety, University of Veterinary Medicine Hannover, Foundation, 30559 Hannover, GermanyInstitute of Clinical Physiology, Department of Gastroenterology, Infectious Diseases and Rheumatology, Charité—Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 12203 Berlin, GermanyInstitute for Food Quality and Food Safety, University of Veterinary Medicine Hannover, Foundation, 30559 Hannover, GermanyInstitute of Clinical Physiology, Department of Gastroenterology, Infectious Diseases and Rheumatology, Charité—Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 12203 Berlin, GermanyInstitute of Microbiology, Infectious Diseases and Immunology, Charité—University Medicine Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 12203 Berlin, GermanyThe polyphenolic compound resveratrol has been shown to exert health-beneficial properties. Given globally emerging <i>Campylobacter</i> infections in humans, we addressed potential anti-pathogenic, immuno-modulatory and intestinal epithelial barrier preserving properties of synthetic resveratrol in the present preclinical intervention study applying a murine acute campylobacteriosis model. Two days following peroral <i>C. jejuni</i> infection, secondary abiotic IL-10<sup>−/−</sup> mice were either subjected to resveratrol or placebo via the drinking water. Whereas placebo mice suffered from acute enterocolitis at day 6 post-infection, resveratrol treatment did not only lead to improved clinical conditions, but also to less pronounced colonic epithelial apoptosis as compared to placebo application. Furthermore, <i>C. jejuni</i> induced innate and adaptive immune cell responses were dampened in the large intestines upon resveratrol challenge and accompanied by less colonic nitric oxide secretion in the resveratrol versus the placebo cohort. Functional analyses revealed that resveratrol treatment could effectively rescue colonic epithelial barrier function in <i>C. jejuni</i> infected mice. Strikingly, the disease-alleviating effects of resveratrol could additionally be found in extra-intestinal and also systemic compartments at day 6 post-infection. For the first time, our current preclinical intervention study provides evidence that peroral resveratrol treatment exerts potent disease-alleviating effects during acute experimental campylobacteriosis.https://www.mdpi.com/2076-2607/8/12/1858polyphenolsresveratrolpreclinical intervention study<i>Campylobacter jejuni</i>secondary abiotic IL-10<sup>−/−</sup> miceacute campylobacteriosis model |
| spellingShingle | Markus M. Heimesaat Soraya Mousavi Ulrike Escher Fábia Daniela Lobo de Sá Elisa Peh Jörg-Dieter Schulzke Sophie Kittler Roland Bücker Stefan Bereswill Resveratrol Alleviates Acute <i>Campylobacter jejuni</i> Induced Enterocolitis in a Preclinical Murine Intervention Study Microorganisms polyphenols resveratrol preclinical intervention study <i>Campylobacter jejuni</i> secondary abiotic IL-10<sup>−/−</sup> mice acute campylobacteriosis model |
| title | Resveratrol Alleviates Acute <i>Campylobacter jejuni</i> Induced Enterocolitis in a Preclinical Murine Intervention Study |
| title_full | Resveratrol Alleviates Acute <i>Campylobacter jejuni</i> Induced Enterocolitis in a Preclinical Murine Intervention Study |
| title_fullStr | Resveratrol Alleviates Acute <i>Campylobacter jejuni</i> Induced Enterocolitis in a Preclinical Murine Intervention Study |
| title_full_unstemmed | Resveratrol Alleviates Acute <i>Campylobacter jejuni</i> Induced Enterocolitis in a Preclinical Murine Intervention Study |
| title_short | Resveratrol Alleviates Acute <i>Campylobacter jejuni</i> Induced Enterocolitis in a Preclinical Murine Intervention Study |
| title_sort | resveratrol alleviates acute i campylobacter jejuni i induced enterocolitis in a preclinical murine intervention study |
| topic | polyphenols resveratrol preclinical intervention study <i>Campylobacter jejuni</i> secondary abiotic IL-10<sup>−/−</sup> mice acute campylobacteriosis model |
| url | https://www.mdpi.com/2076-2607/8/12/1858 |
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