Identification of bacteria and fungi responsible for litter decomposition in desert steppes via combined DNA stable isotope probing
IntroductionSoil microorganisms play crucial roles in determining the fate of litter in desert steppes because their activities constitute a major component of the global carbon (C) cycle. Human activities lead to increased ecosystem nitrogen (N) deposition, which has unpredictable impacts on soil m...
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| Format: | Article |
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Frontiers Media S.A.
2024-03-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1353629/full |
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| author | He Ye He Ye Nare Tu Nare Tu Zhendan Wu Zhendan Wu Shilong He Shilong He Yu Zhao Yu Zhao Mei Yue Mei Yue Mei Hong Mei Hong |
| author_facet | He Ye He Ye Nare Tu Nare Tu Zhendan Wu Zhendan Wu Shilong He Shilong He Yu Zhao Yu Zhao Mei Yue Mei Yue Mei Hong Mei Hong |
| author_sort | He Ye |
| collection | DOAJ |
| description | IntroductionSoil microorganisms play crucial roles in determining the fate of litter in desert steppes because their activities constitute a major component of the global carbon (C) cycle. Human activities lead to increased ecosystem nitrogen (N) deposition, which has unpredictable impacts on soil microorganism diversity and functions. Nowadays, it is necessary to further study the succession of these microorganisms in the process of litter decomposition in desert steppe, and explore the effect of N deposition on this process. This issue is particularly important to resolve because it contributes to the broader understanding of nutrient cycling processes in desert steppes.MethodsIn this study, DNA stable isotope probing (DNA-SIP) was used to study changes in soil bacterial and fungal community composition and function during 8 weeks of culture of 13C-labeled litter in desert steppes.ResultsThe results were as follows: (1) Actinomycetota, Pseudomonadota, and Ascomycota are the main microorganisms involved in litter decomposition in desert steppes; (2) N deposition (50 kg ha−1 year−1) significantly increased the relative abundance of some microorganisms involved in the decomposition process; and (3) N deposition likely promotes litter decomposition in desert steppes by increasing the abundances of N cycles bacteria (usually carrying GH family functional genes).DiscussionThese findings contribute to a deeper understanding of the C assimilation mechanisms associated with litter residue production, emphasizing the importance of extensive C utilization. |
| first_indexed | 2024-04-25T01:42:16Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-25T01:42:16Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-8bf836c80eb54376a2fee5d4f93e60eb2024-03-08T04:44:58ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-03-011510.3389/fmicb.2024.13536291353629Identification of bacteria and fungi responsible for litter decomposition in desert steppes via combined DNA stable isotope probingHe Ye0He Ye1Nare Tu2Nare Tu3Zhendan Wu4Zhendan Wu5Shilong He6Shilong He7Yu Zhao8Yu Zhao9Mei Yue10Mei Yue11Mei Hong12Mei Hong13Key Laboratory of Soil Quality and Nutrient Resources, College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot, ChinaKey Laboratory of Agricultural Ecological Security and Green Development, Universities of Inner Mongolia Autonomous Region, Hohhot, ChinaKey Laboratory of Soil Quality and Nutrient Resources, College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot, ChinaKey Laboratory of Agricultural Ecological Security and Green Development, Universities of Inner Mongolia Autonomous Region, Hohhot, ChinaKey Laboratory of Soil Quality and Nutrient Resources, College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot, ChinaKey Laboratory of Agricultural Ecological Security and Green Development, Universities of Inner Mongolia Autonomous Region, Hohhot, ChinaKey Laboratory of Soil Quality and Nutrient Resources, College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot, ChinaKey Laboratory of Agricultural Ecological Security and Green Development, Universities of Inner Mongolia Autonomous Region, Hohhot, ChinaKey Laboratory of Soil Quality and Nutrient Resources, College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot, ChinaKey Laboratory of Agricultural Ecological Security and Green Development, Universities of Inner Mongolia Autonomous Region, Hohhot, ChinaKey Laboratory of Soil Quality and Nutrient Resources, College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot, ChinaKey Laboratory of Agricultural Ecological Security and Green Development, Universities of Inner Mongolia Autonomous Region, Hohhot, ChinaKey Laboratory of Soil Quality and Nutrient Resources, College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot, ChinaKey Laboratory of Agricultural Ecological Security and Green Development, Universities of Inner Mongolia Autonomous Region, Hohhot, ChinaIntroductionSoil microorganisms play crucial roles in determining the fate of litter in desert steppes because their activities constitute a major component of the global carbon (C) cycle. Human activities lead to increased ecosystem nitrogen (N) deposition, which has unpredictable impacts on soil microorganism diversity and functions. Nowadays, it is necessary to further study the succession of these microorganisms in the process of litter decomposition in desert steppe, and explore the effect of N deposition on this process. This issue is particularly important to resolve because it contributes to the broader understanding of nutrient cycling processes in desert steppes.MethodsIn this study, DNA stable isotope probing (DNA-SIP) was used to study changes in soil bacterial and fungal community composition and function during 8 weeks of culture of 13C-labeled litter in desert steppes.ResultsThe results were as follows: (1) Actinomycetota, Pseudomonadota, and Ascomycota are the main microorganisms involved in litter decomposition in desert steppes; (2) N deposition (50 kg ha−1 year−1) significantly increased the relative abundance of some microorganisms involved in the decomposition process; and (3) N deposition likely promotes litter decomposition in desert steppes by increasing the abundances of N cycles bacteria (usually carrying GH family functional genes).DiscussionThese findings contribute to a deeper understanding of the C assimilation mechanisms associated with litter residue production, emphasizing the importance of extensive C utilization.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1353629/fullDNA-stable isotope probingsoil microorganismsfunctional geneslitter decompositionnitrogen deposition |
| spellingShingle | He Ye He Ye Nare Tu Nare Tu Zhendan Wu Zhendan Wu Shilong He Shilong He Yu Zhao Yu Zhao Mei Yue Mei Yue Mei Hong Mei Hong Identification of bacteria and fungi responsible for litter decomposition in desert steppes via combined DNA stable isotope probing Frontiers in Microbiology DNA-stable isotope probing soil microorganisms functional genes litter decomposition nitrogen deposition |
| title | Identification of bacteria and fungi responsible for litter decomposition in desert steppes via combined DNA stable isotope probing |
| title_full | Identification of bacteria and fungi responsible for litter decomposition in desert steppes via combined DNA stable isotope probing |
| title_fullStr | Identification of bacteria and fungi responsible for litter decomposition in desert steppes via combined DNA stable isotope probing |
| title_full_unstemmed | Identification of bacteria and fungi responsible for litter decomposition in desert steppes via combined DNA stable isotope probing |
| title_short | Identification of bacteria and fungi responsible for litter decomposition in desert steppes via combined DNA stable isotope probing |
| title_sort | identification of bacteria and fungi responsible for litter decomposition in desert steppes via combined dna stable isotope probing |
| topic | DNA-stable isotope probing soil microorganisms functional genes litter decomposition nitrogen deposition |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1353629/full |
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