CDK5RAP3, a Novel Nucleoplasmic Shuttle, Deeply Regulates HSF1-Mediated Heat Stress Response and Protects Mammary Epithelial Cells from Heat Injury

CDK5RAP3 was regarded as the most significant regulator of cellular responses against heat stress, which is associated with dysfunctions of the immune system and animal susceptibility to disease. Despite this, little known about how CDK5RAP3 regulates heat stress response. In this study, CDK5RAP3 co...

Full description

Bibliographic Details
Main Authors: Yangyang Shen, Yan Zou, Jun Li, Fanghui Chen, Honglin Li, Yafei Cai
Format: Article
Language:English
Published: MDPI AG 2020-11-01
Series:International Journal of Molecular Sciences
Subjects:
Online Access:https://www.mdpi.com/1422-0067/21/21/8400
_version_ 1797548442400063488
author Yangyang Shen
Yan Zou
Jun Li
Fanghui Chen
Honglin Li
Yafei Cai
author_facet Yangyang Shen
Yan Zou
Jun Li
Fanghui Chen
Honglin Li
Yafei Cai
author_sort Yangyang Shen
collection DOAJ
description CDK5RAP3 was regarded as the most significant regulator of cellular responses against heat stress, which is associated with dysfunctions of the immune system and animal susceptibility to disease. Despite this, little known about how CDK5RAP3 regulates heat stress response. In this study, CDK5RAP3 conditional Knockout (CKO) mice, CDK5RAP3<sup>-/-</sup> mouse embryo fibroblasts (MEFs) and bovine mammary epithelial cells (BMECs) were used as an in vitro and in vivo model, respectively to reveal the role of CDK5RAP3 in regulating the heat stress response. The deletion of CDK5RAP3 unexpectedly caused animal lethality after 1.5-h heat stimulations. Furthermore, BMECs were re-cultured for eight hours after heat stress and was found that the expression of CDK5RAP3 and HSPs showed a similar fluctuating pattern of increase (0–2, 4–6 h) and decrease (2–4, 6–8 h). In addition to the remarkably enhanced expression of heat shock protein, apoptosis rate and endoplasmic reticulum stress, the deletion of CDK5RAP3 also affected nucleoplasmic translocation and trimer formation of heat shock factor 1 (HSF1). These programs were further confirmed in the mammary gland of CDK5RAP3 CKO mice and CDK5RAP3<sup>-/-</sup> MEFs as well. Interestingly, genetic silencing of HSF1 downregulated CDK5RAP3 expression in BMECs. Immunostaining and immunoprecipitation studies suggested a physical interaction between CDK5RAP3 and HSF1 being co-localized in the cytoplasm and nucleus. Besides, CDK5RAP3 also interacted with HSP90, suggesting an operative machinery at both transcriptional level and protein functionality of HSP90 per se. Together, our findings suggested that CDK5RAP3 works like a novel nucleoplasmic shuttle or molecular chaperone, deeply participating in HSF1-mediated heat stress response and protecting cells from heat injury.
first_indexed 2024-03-10T14:59:56Z
format Article
id doaj.art-8bf9069196fc42ed9217b38e22023677
institution Directory Open Access Journal
issn 1661-6596
1422-0067
language English
last_indexed 2024-03-10T14:59:56Z
publishDate 2020-11-01
publisher MDPI AG
record_format Article
series International Journal of Molecular Sciences
spelling doaj.art-8bf9069196fc42ed9217b38e220236772023-11-20T20:18:02ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-11-012121840010.3390/ijms21218400CDK5RAP3, a Novel Nucleoplasmic Shuttle, Deeply Regulates HSF1-Mediated Heat Stress Response and Protects Mammary Epithelial Cells from Heat InjuryYangyang Shen0Yan Zou1Jun Li2Fanghui Chen3Honglin Li4Yafei Cai5College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Life Sciences, Anhui Normal University, Wuhu 241000, ChinaCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaDepartment of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta University, Augusta, GA 30912, USACollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaCDK5RAP3 was regarded as the most significant regulator of cellular responses against heat stress, which is associated with dysfunctions of the immune system and animal susceptibility to disease. Despite this, little known about how CDK5RAP3 regulates heat stress response. In this study, CDK5RAP3 conditional Knockout (CKO) mice, CDK5RAP3<sup>-/-</sup> mouse embryo fibroblasts (MEFs) and bovine mammary epithelial cells (BMECs) were used as an in vitro and in vivo model, respectively to reveal the role of CDK5RAP3 in regulating the heat stress response. The deletion of CDK5RAP3 unexpectedly caused animal lethality after 1.5-h heat stimulations. Furthermore, BMECs were re-cultured for eight hours after heat stress and was found that the expression of CDK5RAP3 and HSPs showed a similar fluctuating pattern of increase (0–2, 4–6 h) and decrease (2–4, 6–8 h). In addition to the remarkably enhanced expression of heat shock protein, apoptosis rate and endoplasmic reticulum stress, the deletion of CDK5RAP3 also affected nucleoplasmic translocation and trimer formation of heat shock factor 1 (HSF1). These programs were further confirmed in the mammary gland of CDK5RAP3 CKO mice and CDK5RAP3<sup>-/-</sup> MEFs as well. Interestingly, genetic silencing of HSF1 downregulated CDK5RAP3 expression in BMECs. Immunostaining and immunoprecipitation studies suggested a physical interaction between CDK5RAP3 and HSF1 being co-localized in the cytoplasm and nucleus. Besides, CDK5RAP3 also interacted with HSP90, suggesting an operative machinery at both transcriptional level and protein functionality of HSP90 per se. Together, our findings suggested that CDK5RAP3 works like a novel nucleoplasmic shuttle or molecular chaperone, deeply participating in HSF1-mediated heat stress response and protecting cells from heat injury.https://www.mdpi.com/1422-0067/21/21/8400CDK5RAP3HSF1HSP90nucleoplasmic shuttleheat stress response
spellingShingle Yangyang Shen
Yan Zou
Jun Li
Fanghui Chen
Honglin Li
Yafei Cai
CDK5RAP3, a Novel Nucleoplasmic Shuttle, Deeply Regulates HSF1-Mediated Heat Stress Response and Protects Mammary Epithelial Cells from Heat Injury
International Journal of Molecular Sciences
CDK5RAP3
HSF1
HSP90
nucleoplasmic shuttle
heat stress response
title CDK5RAP3, a Novel Nucleoplasmic Shuttle, Deeply Regulates HSF1-Mediated Heat Stress Response and Protects Mammary Epithelial Cells from Heat Injury
title_full CDK5RAP3, a Novel Nucleoplasmic Shuttle, Deeply Regulates HSF1-Mediated Heat Stress Response and Protects Mammary Epithelial Cells from Heat Injury
title_fullStr CDK5RAP3, a Novel Nucleoplasmic Shuttle, Deeply Regulates HSF1-Mediated Heat Stress Response and Protects Mammary Epithelial Cells from Heat Injury
title_full_unstemmed CDK5RAP3, a Novel Nucleoplasmic Shuttle, Deeply Regulates HSF1-Mediated Heat Stress Response and Protects Mammary Epithelial Cells from Heat Injury
title_short CDK5RAP3, a Novel Nucleoplasmic Shuttle, Deeply Regulates HSF1-Mediated Heat Stress Response and Protects Mammary Epithelial Cells from Heat Injury
title_sort cdk5rap3 a novel nucleoplasmic shuttle deeply regulates hsf1 mediated heat stress response and protects mammary epithelial cells from heat injury
topic CDK5RAP3
HSF1
HSP90
nucleoplasmic shuttle
heat stress response
url https://www.mdpi.com/1422-0067/21/21/8400
work_keys_str_mv AT yangyangshen cdk5rap3anovelnucleoplasmicshuttledeeplyregulateshsf1mediatedheatstressresponseandprotectsmammaryepithelialcellsfromheatinjury
AT yanzou cdk5rap3anovelnucleoplasmicshuttledeeplyregulateshsf1mediatedheatstressresponseandprotectsmammaryepithelialcellsfromheatinjury
AT junli cdk5rap3anovelnucleoplasmicshuttledeeplyregulateshsf1mediatedheatstressresponseandprotectsmammaryepithelialcellsfromheatinjury
AT fanghuichen cdk5rap3anovelnucleoplasmicshuttledeeplyregulateshsf1mediatedheatstressresponseandprotectsmammaryepithelialcellsfromheatinjury
AT honglinli cdk5rap3anovelnucleoplasmicshuttledeeplyregulateshsf1mediatedheatstressresponseandprotectsmammaryepithelialcellsfromheatinjury
AT yafeicai cdk5rap3anovelnucleoplasmicshuttledeeplyregulateshsf1mediatedheatstressresponseandprotectsmammaryepithelialcellsfromheatinjury