Occurrence and Genomic Characteristics of Hypervirulent Klebsiella pneumoniae in a Tertiary Care Hospital, Eastern India
Bhuvan Yadav, Srujana Mohanty, Bijayini Behera Department of Microbiology, All India Institute of Medical Sciences, Bhubaneswar, Odisha, 751019, IndiaCorrespondence: Srujana Mohanty, Department of Microbiology, All India Institute of Medical Sciences, Bhubaneswar, Odisha, 751019, India, Tel +9438884...
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| Language: | English |
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Dove Medical Press
2023-04-01
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| Series: | Infection and Drug Resistance |
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| Online Access: | https://www.dovepress.com/occurrence-and-genomic-characteristics-of-hypervirulent-klebsiella-pne-peer-reviewed-fulltext-article-IDR |
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| author | Yadav B Mohanty S Behera B |
| author_facet | Yadav B Mohanty S Behera B |
| author_sort | Yadav B |
| collection | DOAJ |
| description | Bhuvan Yadav, Srujana Mohanty, Bijayini Behera Department of Microbiology, All India Institute of Medical Sciences, Bhubaneswar, Odisha, 751019, IndiaCorrespondence: Srujana Mohanty, Department of Microbiology, All India Institute of Medical Sciences, Bhubaneswar, Odisha, 751019, India, Tel +9438884124, Email srujana_micro@yahoo.co.inPurpose: This study was conducted to find out the occurrence of hypervirulent Klebsiella pneumoniae (hvKP) isolates from different clinical specimens in a tertiary care hospital of eastern India and investigate the distribution of virulence factors, capsular serotypes and antibiogram profile. The distribution of carbapenemase-encoding genes in convergent (hvKP and carbapenem-resistant) isolates was also studied.Materials and methods: A total of 1004 K. pneumoniae isolates were obtained from different clinical specimens from August 2019 to June 2021 and hvKP isolates were identified using the string test. Genes of capsular serotypes K1, K2, K5, K20, K54 and K57, virulence-associated genes, rmpA, rmpA2, mrkD, allS, iroN, iutA, iuc, kfuB and ybtS, and carbapenemase-encoding genes, NDM-1, OXA-48, OXA-181, and KPC, were evaluated by polymerase chain reaction. Antimicrobial susceptibility was determined primarily by the VITEK-2 Compact automated platform (bioMérieux, Marcy-l’Étoile, France) and supplemented by disc-diffusion/EzyMIC (HiMedia, Mumbai, India) wherever needed.Results: Out of 1004 isolates, 33 (3.3%) were hvKP. Most frequent capsular serotype was K2 in 11 (33.3%). Amongst virulence genes, mrkD, iutA and kfuB were detected most frequently in 93.9%, 84.8% and 63.6% isolates respectively. Classical Klebsiella pneumoniae isolates were significantly more resistant than hvKP to cephalosporins, amoxicillin-clavulanic acid, and fluoroquinolones (p < 0.05). Carbapenem resistance was seen in 10 hvKP convergent isolates with the most prevalent carbapenemase-encoding gene being OXA-48 and OXA-181 in 50% isolates.Conclusion: There is a need for continued surveillance of hvKP strains in view of the impending threat of a global spread of convergent strains.Keywords: antimicrobial resistance, carbapenem-resistantK. pneumoniae, hypervirulent Klebsiella pneumoniae, Klebsiella pneumoniae, virulence gene |
| first_indexed | 2024-04-09T18:11:25Z |
| format | Article |
| id | doaj.art-8bf9a1b2848a4f818bbd828efd973087 |
| institution | Directory Open Access Journal |
| issn | 1178-6973 |
| language | English |
| last_indexed | 2024-04-09T18:11:25Z |
| publishDate | 2023-04-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Infection and Drug Resistance |
| spelling | doaj.art-8bf9a1b2848a4f818bbd828efd9730872023-04-13T19:17:09ZengDove Medical PressInfection and Drug Resistance1178-69732023-04-01Volume 162191220183004Occurrence and Genomic Characteristics of Hypervirulent Klebsiella pneumoniae in a Tertiary Care Hospital, Eastern IndiaYadav BMohanty SBehera BBhuvan Yadav, Srujana Mohanty, Bijayini Behera Department of Microbiology, All India Institute of Medical Sciences, Bhubaneswar, Odisha, 751019, IndiaCorrespondence: Srujana Mohanty, Department of Microbiology, All India Institute of Medical Sciences, Bhubaneswar, Odisha, 751019, India, Tel +9438884124, Email srujana_micro@yahoo.co.inPurpose: This study was conducted to find out the occurrence of hypervirulent Klebsiella pneumoniae (hvKP) isolates from different clinical specimens in a tertiary care hospital of eastern India and investigate the distribution of virulence factors, capsular serotypes and antibiogram profile. The distribution of carbapenemase-encoding genes in convergent (hvKP and carbapenem-resistant) isolates was also studied.Materials and methods: A total of 1004 K. pneumoniae isolates were obtained from different clinical specimens from August 2019 to June 2021 and hvKP isolates were identified using the string test. Genes of capsular serotypes K1, K2, K5, K20, K54 and K57, virulence-associated genes, rmpA, rmpA2, mrkD, allS, iroN, iutA, iuc, kfuB and ybtS, and carbapenemase-encoding genes, NDM-1, OXA-48, OXA-181, and KPC, were evaluated by polymerase chain reaction. Antimicrobial susceptibility was determined primarily by the VITEK-2 Compact automated platform (bioMérieux, Marcy-l’Étoile, France) and supplemented by disc-diffusion/EzyMIC (HiMedia, Mumbai, India) wherever needed.Results: Out of 1004 isolates, 33 (3.3%) were hvKP. Most frequent capsular serotype was K2 in 11 (33.3%). Amongst virulence genes, mrkD, iutA and kfuB were detected most frequently in 93.9%, 84.8% and 63.6% isolates respectively. Classical Klebsiella pneumoniae isolates were significantly more resistant than hvKP to cephalosporins, amoxicillin-clavulanic acid, and fluoroquinolones (p < 0.05). Carbapenem resistance was seen in 10 hvKP convergent isolates with the most prevalent carbapenemase-encoding gene being OXA-48 and OXA-181 in 50% isolates.Conclusion: There is a need for continued surveillance of hvKP strains in view of the impending threat of a global spread of convergent strains.Keywords: antimicrobial resistance, carbapenem-resistantK. pneumoniae, hypervirulent Klebsiella pneumoniae, Klebsiella pneumoniae, virulence genehttps://www.dovepress.com/occurrence-and-genomic-characteristics-of-hypervirulent-klebsiella-pne-peer-reviewed-fulltext-article-IDRantimicrobial resistancecarbapenem-resistant k. pneumoniaehypervirulent klebsiella pneumoniaeklebsiella pneumoniaevirulence gene |
| spellingShingle | Yadav B Mohanty S Behera B Occurrence and Genomic Characteristics of Hypervirulent Klebsiella pneumoniae in a Tertiary Care Hospital, Eastern India Infection and Drug Resistance antimicrobial resistance carbapenem-resistant k. pneumoniae hypervirulent klebsiella pneumoniae klebsiella pneumoniae virulence gene |
| title | Occurrence and Genomic Characteristics of Hypervirulent Klebsiella pneumoniae in a Tertiary Care Hospital, Eastern India |
| title_full | Occurrence and Genomic Characteristics of Hypervirulent Klebsiella pneumoniae in a Tertiary Care Hospital, Eastern India |
| title_fullStr | Occurrence and Genomic Characteristics of Hypervirulent Klebsiella pneumoniae in a Tertiary Care Hospital, Eastern India |
| title_full_unstemmed | Occurrence and Genomic Characteristics of Hypervirulent Klebsiella pneumoniae in a Tertiary Care Hospital, Eastern India |
| title_short | Occurrence and Genomic Characteristics of Hypervirulent Klebsiella pneumoniae in a Tertiary Care Hospital, Eastern India |
| title_sort | occurrence and genomic characteristics of hypervirulent klebsiella pneumoniae in a tertiary care hospital eastern india |
| topic | antimicrobial resistance carbapenem-resistant k. pneumoniae hypervirulent klebsiella pneumoniae klebsiella pneumoniae virulence gene |
| url | https://www.dovepress.com/occurrence-and-genomic-characteristics-of-hypervirulent-klebsiella-pne-peer-reviewed-fulltext-article-IDR |
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