Tyrosine Kinase Self-Phosphorylation Controls Exopolysaccharide Biosynthesis in <i>Gluconacetobacter diazotrophicus</i> Strain Pal5
The biosynthesis of exopolysaccharides (EPSs) is essential for endophytic bacterial colonisation in plants bacause this exopolymer both protects bacterial cells against the defence and oxidative systems of plants and acts on the plant colonisation mechanism in <i>Gluconacetobacter diazotrophic...
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MDPI AG
2021-11-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/11/11/1231 |
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| author | Katyanne Wanderley Dayse Sousa Gabriel Silva Josemir Maia Maria Silva Marcia Vidal José Baldani Carlos Meneses |
| author_facet | Katyanne Wanderley Dayse Sousa Gabriel Silva Josemir Maia Maria Silva Marcia Vidal José Baldani Carlos Meneses |
| author_sort | Katyanne Wanderley |
| collection | DOAJ |
| description | The biosynthesis of exopolysaccharides (EPSs) is essential for endophytic bacterial colonisation in plants bacause this exopolymer both protects bacterial cells against the defence and oxidative systems of plants and acts on the plant colonisation mechanism in <i>Gluconacetobacter diazotrophicus</i>. The pathway involved in the biosynthesis of bacterial EPS has not been fully elucidated, and several areas related to its molecular regulation mechanisms are still lacking. <i>G. diazotrophicus</i> relies heavily on EPS for survival indirectly by protecting plants from pathogen attack as well as for endophytic maintenance and adhesion in plant tissues. Here, we report that EPS from <i>G. diazotrophicus</i> strain Pal5 is a signal polymer that controls its own biosynthesis. EPS production depends on a bacterial tyrosine (BY) kinase (Wzc) that consists of a component that is able to phosphorylate a glycosyltranferase or to self-phosphorylate. EPS interacts with the extracellular domain of Wzc, which regulates kinase activity. In <i>G. diazotrophicus</i> strains that are deficient in EPS production, the Wzc is rendered inoperative by self-phosphorylation. The presence of EPS promotes the phosphorylation of a glycosyltransferase in the pathway, thus producing EPS. Wzc-mediated self-regulation is an attribute for the control of exopolysaccharide biosynthesis in <i>G. diazotrophicus</i>. |
| first_indexed | 2024-03-10T05:20:51Z |
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| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-10T05:20:51Z |
| publishDate | 2021-11-01 |
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| series | Life |
| spelling | doaj.art-8c00169c9bea4d34802a7546bfe559132023-11-23T00:04:47ZengMDPI AGLife2075-17292021-11-011111123110.3390/life11111231Tyrosine Kinase Self-Phosphorylation Controls Exopolysaccharide Biosynthesis in <i>Gluconacetobacter diazotrophicus</i> Strain Pal5Katyanne Wanderley0Dayse Sousa1Gabriel Silva2Josemir Maia3Maria Silva4Marcia Vidal5José Baldani6Carlos Meneses7Programa de Pós-Graduação em Ciências Agrárias, Campus I, Universidade Estadual da Paraíba, Campina Grande 58429-500, BrazilPrograma de Pós-Graduação em Ciências Agrárias, Campus I, Universidade Estadual da Paraíba, Campina Grande 58429-500, BrazilCentro de Ciências Biológicas e da Saúde, Departamento de Biologia, Campus I, Universidade Estadual da Paraíba, Campina Grande 58429-500, BrazilPrograma de Pós-Graduação em Ciências Agrárias, Campus I, Universidade Estadual da Paraíba, Campina Grande 58429-500, BrazilCentro de Ciências Biológicas e da Saúde, Departamento de Biologia, Campus I, Universidade Estadual da Paraíba, Campina Grande 58429-500, BrazilEmbrapa Agrobiologia, Rodovia BR 465, km 07, Seropédica 23891-000, BrazilEmbrapa Agrobiologia, Rodovia BR 465, km 07, Seropédica 23891-000, BrazilPrograma de Pós-Graduação em Ciências Agrárias, Campus I, Universidade Estadual da Paraíba, Campina Grande 58429-500, BrazilThe biosynthesis of exopolysaccharides (EPSs) is essential for endophytic bacterial colonisation in plants bacause this exopolymer both protects bacterial cells against the defence and oxidative systems of plants and acts on the plant colonisation mechanism in <i>Gluconacetobacter diazotrophicus</i>. The pathway involved in the biosynthesis of bacterial EPS has not been fully elucidated, and several areas related to its molecular regulation mechanisms are still lacking. <i>G. diazotrophicus</i> relies heavily on EPS for survival indirectly by protecting plants from pathogen attack as well as for endophytic maintenance and adhesion in plant tissues. Here, we report that EPS from <i>G. diazotrophicus</i> strain Pal5 is a signal polymer that controls its own biosynthesis. EPS production depends on a bacterial tyrosine (BY) kinase (Wzc) that consists of a component that is able to phosphorylate a glycosyltranferase or to self-phosphorylate. EPS interacts with the extracellular domain of Wzc, which regulates kinase activity. In <i>G. diazotrophicus</i> strains that are deficient in EPS production, the Wzc is rendered inoperative by self-phosphorylation. The presence of EPS promotes the phosphorylation of a glycosyltransferase in the pathway, thus producing EPS. Wzc-mediated self-regulation is an attribute for the control of exopolysaccharide biosynthesis in <i>G. diazotrophicus</i>.https://www.mdpi.com/2075-1729/11/11/1231plant growth-promoting bacteriaexopolymer productionbacterial protein phosphorylationautophosphorylation |
| spellingShingle | Katyanne Wanderley Dayse Sousa Gabriel Silva Josemir Maia Maria Silva Marcia Vidal José Baldani Carlos Meneses Tyrosine Kinase Self-Phosphorylation Controls Exopolysaccharide Biosynthesis in <i>Gluconacetobacter diazotrophicus</i> Strain Pal5 Life plant growth-promoting bacteria exopolymer production bacterial protein phosphorylation autophosphorylation |
| title | Tyrosine Kinase Self-Phosphorylation Controls Exopolysaccharide Biosynthesis in <i>Gluconacetobacter diazotrophicus</i> Strain Pal5 |
| title_full | Tyrosine Kinase Self-Phosphorylation Controls Exopolysaccharide Biosynthesis in <i>Gluconacetobacter diazotrophicus</i> Strain Pal5 |
| title_fullStr | Tyrosine Kinase Self-Phosphorylation Controls Exopolysaccharide Biosynthesis in <i>Gluconacetobacter diazotrophicus</i> Strain Pal5 |
| title_full_unstemmed | Tyrosine Kinase Self-Phosphorylation Controls Exopolysaccharide Biosynthesis in <i>Gluconacetobacter diazotrophicus</i> Strain Pal5 |
| title_short | Tyrosine Kinase Self-Phosphorylation Controls Exopolysaccharide Biosynthesis in <i>Gluconacetobacter diazotrophicus</i> Strain Pal5 |
| title_sort | tyrosine kinase self phosphorylation controls exopolysaccharide biosynthesis in i gluconacetobacter diazotrophicus i strain pal5 |
| topic | plant growth-promoting bacteria exopolymer production bacterial protein phosphorylation autophosphorylation |
| url | https://www.mdpi.com/2075-1729/11/11/1231 |
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