Exo84c-regulated degradation is involved in the normal self-incompatible response in Brassicaceae
Summary: The self-incompatibility system evolves in angiosperms to promote cross-pollination by rejecting self-pollination. Here, we show the involvement of Exo84c in the SI response of both Brassica napus and Arabidopsis. The expression of Exo84c is specifically elevated in stigma during the SI res...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124724002419 |
| _version_ | 1797272497219960832 |
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| author | Tong Zhang Kun Wang Shengwei Dou Erlin Gao Patrick J. Hussey Zongcheng Lin Pengwei Wang |
| author_facet | Tong Zhang Kun Wang Shengwei Dou Erlin Gao Patrick J. Hussey Zongcheng Lin Pengwei Wang |
| author_sort | Tong Zhang |
| collection | DOAJ |
| description | Summary: The self-incompatibility system evolves in angiosperms to promote cross-pollination by rejecting self-pollination. Here, we show the involvement of Exo84c in the SI response of both Brassica napus and Arabidopsis. The expression of Exo84c is specifically elevated in stigma during the SI response. Knocking out Exo84c in B. napus and SI Arabidopsis partially breaks down the SI response. The SI response inhibits both the protein secretion in papillae and the recruitment of the exocyst complex to the pollen-pistil contact sites. Interestingly, these processes can be partially restored in exo84c SI Arabidopsis. After incompatible pollination, the turnover of the exocyst-labeled compartment is enhanced in papillae. However, this process is perturbed in exo84c SI Arabidopsis. Taken together, our results suggest that Exo84c regulates the exocyst complex vacuolar degradation during the SI response. This process is likely independent of the known SI pathway in Brassicaceae to secure the SI response. |
| first_indexed | 2024-03-07T14:30:12Z |
| format | Article |
| id | doaj.art-8c019d9d881c4f9e8b9e1c324e82d313 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-03-07T14:30:12Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-8c019d9d881c4f9e8b9e1c324e82d3132024-03-06T05:26:36ZengElsevierCell Reports2211-12472024-03-01433113913Exo84c-regulated degradation is involved in the normal self-incompatible response in BrassicaceaeTong Zhang0Kun Wang1Shengwei Dou2Erlin Gao3Patrick J. Hussey4Zongcheng Lin5Pengwei Wang6National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China; College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271018, China; Hubei Hongshan Laboratory, Wuhan 430070, ChinaNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Hubei Hongshan Laboratory, Wuhan 430070, ChinaCollege of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271018, China; Hubei Hongshan Laboratory, Wuhan 430070, China; National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, ChinaNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China; Hubei Hongshan Laboratory, Wuhan 430070, ChinaDepartment of Biosciences, Durham University, South Road, DH1 3LE Durham, UKNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China; Hubei Hongshan Laboratory, Wuhan 430070, ChinaNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Hubei Hongshan Laboratory, Wuhan 430070, China; Corresponding authorSummary: The self-incompatibility system evolves in angiosperms to promote cross-pollination by rejecting self-pollination. Here, we show the involvement of Exo84c in the SI response of both Brassica napus and Arabidopsis. The expression of Exo84c is specifically elevated in stigma during the SI response. Knocking out Exo84c in B. napus and SI Arabidopsis partially breaks down the SI response. The SI response inhibits both the protein secretion in papillae and the recruitment of the exocyst complex to the pollen-pistil contact sites. Interestingly, these processes can be partially restored in exo84c SI Arabidopsis. After incompatible pollination, the turnover of the exocyst-labeled compartment is enhanced in papillae. However, this process is perturbed in exo84c SI Arabidopsis. Taken together, our results suggest that Exo84c regulates the exocyst complex vacuolar degradation during the SI response. This process is likely independent of the known SI pathway in Brassicaceae to secure the SI response.http://www.sciencedirect.com/science/article/pii/S2211124724002419CP: PlantsCP: Cell biology |
| spellingShingle | Tong Zhang Kun Wang Shengwei Dou Erlin Gao Patrick J. Hussey Zongcheng Lin Pengwei Wang Exo84c-regulated degradation is involved in the normal self-incompatible response in Brassicaceae Cell Reports CP: Plants CP: Cell biology |
| title | Exo84c-regulated degradation is involved in the normal self-incompatible response in Brassicaceae |
| title_full | Exo84c-regulated degradation is involved in the normal self-incompatible response in Brassicaceae |
| title_fullStr | Exo84c-regulated degradation is involved in the normal self-incompatible response in Brassicaceae |
| title_full_unstemmed | Exo84c-regulated degradation is involved in the normal self-incompatible response in Brassicaceae |
| title_short | Exo84c-regulated degradation is involved in the normal self-incompatible response in Brassicaceae |
| title_sort | exo84c regulated degradation is involved in the normal self incompatible response in brassicaceae |
| topic | CP: Plants CP: Cell biology |
| url | http://www.sciencedirect.com/science/article/pii/S2211124724002419 |
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